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Evaluation of the effect of local exogenous application of osteopontin on wound healing in rats

Authors: Zahraa Raheem Al-Zamely زهراء رحيم الزاملي --- Enas Fadhil Kadhim ايناس فاضل كاظم
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2018 Volume: 30 Issue: 2 Pages: 23-28
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Wound healing is a complex dynamical interaction between various cell types, the extracellular matrix, cytokines, and growth factors. osteoponetin is a substance that acts as an anti-inflammatory.Aims of study: The study was designed to identify the role of local exogenous applications of osteopontin on wound healing (in cheek skin).Materials and methods: Thirty adult male albino rats weighting an average of (250-300gm) used in this study, incisional wounds were made in the skin of the cheek of rat and they were divided into the following groups:A-Control group: 15 rats treated with 1μ l of normal salineB-Experimental groups: 15 rats treated with topical application of 1μl osteopontin.The scarification of animals were done for the healing intervals (1, 5 and 10 days) Histological analysis and assessment of number of inflammatory cell, Thickness and contraction of incision were performed for both experimental and control groups for all healing periods.Results: Histological analysis revealed that osteopontin accelerate wound healing of cheek skin and there was highly significant difference among studied groups in periods 1,5,10 days. Regarding mean values of epithelial thickness, inflammatory cell count and contraction of wound area have reported a highest value different in 1, 5 and 10 days durations.Conclusion: It can be concluded that application of osteopontin shown efficacy in the healing of skin wounds induced in rats


Article
Analysis of inflammatory cells in osseointegration of CpTi implant radiated by low level laser therapy

Author: Eman I. Al- Tamemi ايمان التميمي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 1 Pages: 105-110
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Dental implants provide a unique treatment modality for the replacement of a lost dentition .This isaccomplished by the insertion of relatively an inert material (a biomaterial) into the soft and hard tissue of the jaws,there by providing support and retention for dental prostheses. Low level laser therapy (LLLT) is an effective tool usedto prompt bone repair and remodeling, this has referred to the biostimulation effect of LLLT. The Aim of this study wasto evaluate the effects of inflammatory cells on osseointegration of CpTi implant irradiated by low level laser.Materials and Methods: thirty two adult New Zealand white rabbits, received titanium implants were inserted in thetibia. The right side is considered as experimental groups and the left side considered as control groups. Low powerdiode laser (GaAlAs ) with wave length (904nm) and (5mW)power applicated with the right implants . The sampledivided into four groups, eight rabbits were sacrificed at four interval 4days, 1 weeks, 2weeks, and 6weeksrespectively. Histological and inflammatory analyses were done for each interval.Results: Histological examination showed acceleration of bone formation and more rapid healing process in thescrew implant with laser irradiation than in the control implant .inflammatory analysis showed dramatic decrease withthe presence of laser irradiation especially with advancing time.Conclusion: This study illustrated that the inflammatory cells were reduced in osseointegration of dental implanttreated with LLLT.

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