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Molecular Detection of Some Virulence Genes in Klebsiella pneumoniae Isolates from Patients with UTI Infection.

Author: Saba Saadoon Khazaal
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 4 Pages: 701 -705
Publisher: Babylon University جامعة بابل

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Abstract

Klebsiella pneumoniae causes a wide range of bacterial diseases like pneumonia, UTI and sepsis. Therefore, this study was done to assess the prevalence and molecular characteristics of K. pneumoniae in 24 samples of men and women isolated from Iraqi patients which was suffering from urinary tract infection (UTI) compared with (10) of healthy individuals. All samples were collected from Educational laboratories of Medical city/Iraqi Health Ministry. The samples were screened for the presence of kfu and k2A genes through PCR. The results of this study represented by (7 out of 24) isolates percent with (29.17%) positive for kfu gene and (9 out of 24) isolates (37.5%) were positive for k2A gene while the other (8) isolates were none of these genes. The two genes revealed significant differences between them (p< 0.5), so as with healthy group (p< 0.01). The study concluded that two genes kfu and k2A may affect on pathological of K. pneumoniae. The aim of this study represented by detection of kfu (iron uptake system gene) and k2A genes in patients with UTI which have a role in the pathogenicity of K. pneumoniae isolates.

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