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Ki-67(MIB-1) and Progesterone Receptor in Meningioma An Immunohistochemical Study

Author: Wahda Mohammed Taib Al- Nuaimy
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 2 Pages: 157-167
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: In meningioma the tumor grade, subtype, and extent of surgical resection are strong prognostic factors, the growth of this tumor is still unpredictable, and additional prognostic markers are needed. Many studies have shown that the detection of the proliferative potential of meningioma by Ki-67 (MIB-1) and the detection of the progesterone receptor might predict the natural history of tumor and patient survival. OBJECTIVE: To evaluate the proliferative fraction Ki-67 (MIB-1) and progesterone receptor in meningioma, To correlate the expression of these two markers with various clinico- pathological parameters, To compare these results with other studies . PATIENTS AND METHODS: This is a prospective study, in which 50 cases of meningioma were collected in Nineveh province. These cases were examined immunohistochemically. The results of these two markers were correlated with various clinico- pathological parameters. RESULTS: The mean age of all cases was 46 years. There were 16 male and 34 female patients. According to the W.H.O 2000 classification, there were 84% grade I, 10% were grade II and 6 %were grade III. Only 14% of the patients had a history of recurrence. Progesterone receptor was positive in 72% of all cases. Significant relation was observed between the progesterone receptor and the patients age .It was significantly higher in female than male. A significant relationship was found between progesterone receptor with grade and histological types. Thirty three out of 43 cases without recurrence had positive progesterone receptor. The mean±Standard deviation (SD) of Ki-67 Labeling Index (Ki-67 LI) was 2.7±4.2%. No significant relation was found between the Ki-67 LI and patients age. The male patients had a higher Ki-67 LI than that of the female. Significant relation was found between Ki-67 LI and the grade of meningioma . The mean of Ki-67 LI in the recurrent cases was higher than in non recurrent cases. An inverse relationship was found between Ki-67 LI and progesterone receptor . CONCLUSION: Progesterone receptor showed a significant relation with the age and histological types. Ki-67 LI had a direct significant association with the grade and the recurrent cases., while inverse relation was observed between the progesterone receptor with the grade and the recurrent cases. An inverse relationship was observed between the progesterone receptor and Ki-67 LI.


Article
P53 and PCNA overexpression in papillary urothelial tumors: an immunohistochemical study
التعبير المفرط لعامل ((P53 وعامل التكاثر النووي في أورام المثانة الانتقالية الحليميه )دراسة مناعية نسيجية كيميائية (

Author: Hadeel A. Kerbel, M.B.Ch.B, FICMS (Path). هديل عبد الإله كربل
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2011 Volume: 14 Issue: 1 Pages: 236-244
Publisher: University of Kufa جامعة الكوفة

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Abstract

Objective: The aim of the present study was to evaluate the expression of P53 protein and PCNA and their correlation with different papillary grades of urothelial tumors according to WHO/ISUP classification.Methods: Thirty five cases of urothelial tumors and their corresponding paraffin blocks from 2009-2010 were submitted in this study in the Department of Pathology, College of Medicine, Kufa University. Ten biopsies of benign urothelium (cystitis) were considered as control group. ABC method was used to determine the expression of p53 and PCNA in these cases, Two hundred cells were counted and the percentage of cells positive for p53 and PCNA (labeling index [LI]) was counted. Immunohistochemical positivity was defined as strong, homogenous nuclear staining. Statistically Chi square, Fisher exact probability and correlation co-efficient tests were used by the help of SPSS version 10. Results: P53 expression was detected in 20 cases of TCC with mean LI of 27.4%, while PCNA were detected in 24 samples out of 35 with mean LI of 48.2% with significant levels of expression between malignant and benign urothelium (P<0.05). Regarding p53 expression, none of the benign lesions show positive results, while it was observed in 1 of 5 for papilloma with mean labeling index LI of 2% and 5 of 12 for LMP with LI 14.3%. In contrast, p53 was a feature of papillary carcinoma it expressed in 8 of 11low- grade and 6 of 7 high grade papillary urothelial carcinoma with mean LIs of 36.8% and 53.4% respectively. PCNA positivity was as follow: benign urothelium (mean LI 3.5%), papilloma( mean LI 25%), LMP tumors (mean LI31.6%), low grade papillary carcinoma(mean LI 63.3%), and high grade papillary carcinoma(mean LI 69%). PCNA expression separate benign urothelium and papilloma from tumors of LMP, low and high grade papillary carcinoma.Conclusion:An increased in proliferative index as demonstrated by immunohistochemical staining for PCNA is most often seen in papillary carcinoma and tumors of LMP. While p53 positivity is mainly a feature of low grade and high grade papillary carcinoma.

الهدف: لدراسة التعبير المناعي لعاملp53 وعامل التكاثر النووي PCNAفي ورم المثانة الانتقالي ألحليمي وعلاقتهما مع مختلف درجات التمايز لهذا الورم.الطريقه: تم الحصول على خمس وثلاثين نموذج لنسيج المثانة الانتقالي ألحليمي مثبته بالفورمالين ومطمورة بالبارافين للفترة مابين 2009-2010 تم اجراء هذه الدراسه في فرع الامراض , كلية الطب في جامعة الكوفه. كما تم الحصول على 10 نماذج لنسيج المثانة الحميد ( التهاب المثانه) حيث استخدمت كمجموعه قياسيه. استخدمت طريقة ABC لتحديد التعبير المناعي النسيجي لعاملي p53 وPCNA . حيث تم احتساب 200خلية ورميه وتم استخراج النسبه المئويه للخلايا الموجبه الصبغه وتم التعبير عن هذه النسبه من خلال حساب معدل عامل التأشيرlabeling index.النتائج: أظهرت النتائج ان التعبير المفرط لعامل p53 كان موجبا في 20 حاله (LI 27.4%) في حين ان عامل PCNA كان موجبا في 24 حاله (LI 4802%) وارتباطهما بعلاقة ايجابيه مقارنة بنسيج المثانه الحميد (p<0.05). بالنسبه لعامل p53 , لم يظهر نسيج المثانه الحميد أي نتائج موجبه في حين كانت شدة ظهوره في ورم المثانة الحليمي كالتالي: ورم المثانه الحليمي الحميد نتيجه واحدة موجبة من اصل 5 حالات, ورم المثانة قليل الدرجه السرطانيه 5 من اصل 12 حاله, سرطان المثانه الحليمي واطئ الدرجة 8 من اصل 11 حاله وسرطان المثاني الحليمي عالي الدرجه 6 من اصل 7 حالات. في حين كانت شدة ظهور PCNA كالتالي: نسيج المثانه الحميد (LI 3.5%), ورم المثانه الحليمي الحميد (LI 25%) ورم المثانة الحليمي الحميد واطئ الدرجه(LI31.6%) , سرطان المثانه الحليمي واطئ الدرجه (LI63.3%), سرطان المثانة الحليمي عالي الشده .(LI 69%) المناقشات: من خلال النتائج السابقة نستنتج ان زيادة مؤشر التكاثرالذي اظهره عامل التكاثر النووي PCNA هو صفه مميزة لسرطان المثانة الانتقالي الحليمي وكذلك لورم المثانه الحليمي الحميد واطئ الدرجة. في حين كان ظهور عامل P53 صفه مميزه لسرطان المثانة الانتقالي الحليمي بدرجتيه الواطئة والعالية.

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