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Molecular feature of lasB gene of Pseudomonas aeruginosa isolated from different clinical sources

Author: Molecular feature of lasB gene of Pseudomonas aeruginosa isolated from different clinical sources
Journal: JOURNAL OF THI-QAR SCIENCE مجلة علوم ذي قار ISSN: 19918690 Year: 2018 Volume: 6 Issue: 4 Pages: 163-166
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

The study was carried out through a period from June to November 2017 from out-patients and in-patients of Al-Hussein Teaching Hospital in Thi-Qar province South of Iraq. A sum of 132 specimens from the hospital facility condition and different clinical samples of various patients were randomly collected and analyzed for diagnosis of Pseudomonas aeruginosa. These clinical specimens were included wound, burn, ear swabs, urine and sputum specimens. Every single specimen was screened to detect of P. aeruginosa by culturing on suitable media, and 36 isolates of P. aeruginosa were distinguished through biochemical tests and using API 20NE system for confirmation the isolates. All P. aeruginosa isolates were tested against nine antimicrobial discs in vitro. They revealed that having different classes and the results demonstrated that variable resistance to the anti-microbial agents. In attempting to the recognizable proof of P. aeruginosa having the DNA, Polymerase chain reaction (PCR) was utilized in light of particular groundwork for 16S rRNA genetic marker. The results demonstrated that PCR has observed to be quick and sensitive and particular for recognizable proof of P. aeruginosa. Furthermore, 16S rRNA was utilized as confirmation feature, while lasB detected as virulence gene also to utilize as pathogenesis quality of the bacteria


Article
DNA Sequences of LasB Gene in Pseudomonas aeruginosa Isolated from Some Clinical Cases
التسلسل التتابعي لجين LasB في بكترياPseudomonas aeruginosa المعزولة من بعض الحالات السريرية

Author: Rana M. A. Al-Shwaikh رنا مجاهد عبدالله الشويخ
Journal: Ibn Al-Haitham Journal For Pure And Applied Science مجلة ابن الهيثم للعلوم الصرفة والتطبيقية ISSN: 16094042 Year: 2016 Volume: 29 Issue: 1 Pages: 366-376
Publisher: Baghdad University جامعة بغداد

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Abstract

Out of 120 isolates from different clinical cases, only 75 were found and confirmed that they belong to the Pseudomonas aeruginosa bacteria. The result revealed that the LasB virulent gene was present in 63 isolates with 63% percentage. The gel electrophoresis showed that the molecular weight of LasB gene was 300 bp. DNA sequences of LasB gene was done, and the results showed the presence of some gene mutations like substitution, addition and deletion with 97% identity with the Refseq gene. From the other side, the results of identities of translated nucleotides sequence with the original sequence of amino acids revealed that there are no effects of gene mutations on translation of the product protein.

حصل على 75عزلة تعود الى بكتريا Pseudomonas aeruginosa من اصل 120 عينة جمعت من حالات مرضية مختلفة. أظهرت نتائج التحري عن جين الضراوة لانزيم Elastase (Pseudolysin ) LasB في بكتيريا P. aeruginosa الى وجود هذا الجين في 63 عزلة وبنسبة 84% وكان الحجم الجزيئي للجين 300 زوج قاعدة بعد ترحيله كهربائيا. حلل التسلسل التتابعي لجين LasB في بكتريا P. aeruginosa . بينت النتائج ان هنالك طفرات وراثية في دنا الجين LasB ، حدثت هذه الطفرات في تسلسل القواعد النتروجينية وكان اغلبها من نوع الاستبدال والاضافة والحذف وكانت نسبة التطابق 97 % مع التسلسل التتابعي للجين الاصلي ، كذلك تبين من مطابقة التسلسل التتابعي للقواعد النتروجينية المترجمة مع تسلسل الحامض الاميني الذي يشفر عن هذا الجين ، لم يكن للطفرات تأثير في نتائج ترجمة البروتين وذلك لكونها طفرات صامتة.

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