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Article
Diagnosis of the Problems Which Facing the Researchers during Studying In vitro Lead Toxicity

Author: Sabah Hussain Enayah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 1 Pages: 5-11
Publisher: Babylon University جامعة بابل

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Abstract

Background: Lead (Pb) is one of the most difficult metals to investigate in laboratory experiments because it is very easilyprecipitates or forms complex ions. Many experiments were conducted with Pb and observed unique chemical properties of this metal.Objective: The present investigation was designed to evaluate the cytotoxicity of Pb exposer on pheochromocytoma (PC12) cells indifferent condition and media to admit the difficulties facing the researchers in bioavailability of Pb in media and prevent precipitationformation. Materials and Methods: Pb from 0.01 to 100 μM had been used on PC12 cells cultured and treated in different conditions for 6,12, 24, and 48 h. Resazurin assay was used to assess the cell viability. Result: As numerous studies have demonstrated a possible mechanismfor Pb‑induced neurotoxicity using in vitro model, the current study is the first study which explained the complexity of Pb participations inmedia that facing the authors and what the reason for that and how we can solve this problem to make pb more bioavailable in the media.Conclusion: Use of phosphate buffer in experiments with Pb is not acceptable because all Pb can be precipitated. However, it could be possibleto use the standard media, but for the time of treatment with Pb phosphates must be removed from the media.


Article
Bioremediation of Nickel and Lead contaminated soil by Vica faba L. plant and AM fungi Glomus mosseae
الأستصلاح البايولوجي للترب الملوثة بالنيكل والرصاص بأستعمال نبات الباقلاء .L Vicia fabaوفطر المايكورايزا Glomus mosseae

Authors: Dina A. Al-Maliki --- Ali H. Al-Mousawi
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2015 Volume: 12 Issue: 2 Pages: 260-265
Publisher: Baghdad University جامعة بغداد

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Abstract

This study is conducted to determine the activity of plant Vica faba and two isolated from arbuscular mycorrhizae fungi (A,B) in bioremediation of soil pollution by Nickel and Lead elements in north and south of Baghdad city. The results showed that the average of soil pollution by Nickel and Lead elements in north of Baghdad was less than the average of soil pollution in the south of Baghdad which recorded 29.0,9.0PPm and 42.0, 25.0PPm respectively. The results show that the isolate A from the polluted soil is more active from isolate B which isolate from unpolluted soil for bioremediation. Vica faba recorded more in accumulate the Lead element in shoot system which was 19.65PPm and in root system was 27.2PPm and for Nickel element 24.65, 27.55PPm in shoot and root respectively.

نفذت هذه الدراسة لتحديد فعالية نبات الباقلاء Vicia faba وعزلتين من فطر المايكورايزا الشجيرية Glomus mosseae (A,B) في الاستصلاح البايولوجي للترب الملوثة بعنصري النيكل والرصاص في شمال وجنوب مدينة بغداد.أظهرت نتائج الدراسة أن معدلات تلوث التربة بعنصري النيكل والرصاص في شمال مدينة بغداد كانت أقل من معدلات تلوث التربة جنوب بغداد اذ سجلت 29.0 , 9.0 PPm و 42.0 , 25.0 PPm وعلى التوالي. كما أظهرت النتائج تفوق العزلةA المعزولة من تربة ملوثة على العزلة B المعزولة من ترب غير ملوثة للفطر Glomus mosseae في قابليتها على الاستصلاح البايولوجي , وسجل نبات الباقلاء قابلية على تجميع عنصر الرصاص في المجموع الخضري بلغPPm 19.65 وفي المجموع الجذري 27.2 PPm ولعنصر النيكل 24.65 , 27.55 PPm للمجموعين الجذري والخضري وعلى التوالي.

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