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Article
SOME HAEMATOLOGICAL VALUES IN NORMAL DOGS
دراسة بعض القيم الدمية للكلاب الطبيعية

Author: Rahman Kadhum Muhsen Saleem Amin Hasso* رحمن كاظم محسن* , سليم أمين حسو**
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2008 Volume: 7 Issue: 1 Pages: 46-50
Publisher: Basrah University جامعة البصرة

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Abstract

Haematological values of sixty normal dogs aged from 2 months to 2 years of both sexes were estimated. Red blood cells count (RBCs), hemoglobin concentration (Hb), packed cell volume (PCV). Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), total and differential leukocyte count, total plasma protein, plasma albumin, fibrinogen and globulins were determined using several laboratory techniques and equations.The results revealed that, all RBCs parameters decreased with age except Hb, MCH and MCHC. The latter two increased with age while Hb was unchanged. All other parameters increased significantly with age. There were no significant differences in all parameters between both sexes.

تم قياس الافيام الدمية لستين كلبا سليما من الناحية السريرية تراوحت أعمارها بين شهرين الى سنتين وشملت الفحوصات الدمية : حساب عدد كريات الدم الحمر ، تركيز الهيموكلوبين ، حجم الخلايا المرصوص ، معدل الحجم الكريي ، معدل خضاب الدم الكريي ، معدل تركيز خضاب الدم الكريي ، حساب العدد الكلي والتفريقي لكريات الدم البيض وحساب تركيز بروتينات بلازما الدم وذلك باستعمال عدة تقنيات مختبرية مع تطبيق بعض المعادلات الحسابية . أظهرت النتائج إن معايير كريات الدم الحمر تتناقص معنويا مع تقدم العمر عدا تركيز الهيموكلوبين، معدل خضاب الدم الكريي و معدل تركيز خضاب الدم الكريي حيث ازداد المعياران الاخيران معنويا مع تقدم العمر بينما لم يطرأ اي تغيير على تركيز الهيموكلوبين بتقدم العمر . اما المعايير الاخرى فقد ازدادت معنويا بتقدم العمر . ولم تظهر النتائج وجود أي فرقا معنويا في جميع المعايير بين كلا الجنسين

Keywords

Fibrinogen --- MCV --- Leukocyte


Article
STUDY THE EFFECT OF AGE AND SEX ON SOMEHAEMATOLOGICAL PARAMETERS IN GOLDEN LOCAL QUAIL
دراسة تأثير العمر والجنس على بعض المعايير الدموية في السمان المحلي الذهبي

Author: Muhammad H.K محمد حميد خلف
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2013 Volume: 12 Issue: 1 Pages: 135-141
Publisher: Basrah University جامعة البصرة

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Abstract

Effect of age and sex were measured during and before sexual maturation ofgolden local quail males and females. Maels erythrocytes count showed higher valuecompared with that of adult females . Haemoglobin and haematocrit of adult maleswere significantly higher than those of adult females. However total leukocyte countand H/L ratio of the adult female were higher than those of males. Body weight ofboth sexes increased with age. As well as many of the haematological parametersdiffered significantly (P<0.05) in accordance to the age and sex .


Article
HLA Class I and Class II Polymorphisms and Anti-nuclear Antibodies in Hyperprolactinaemic Iraqi Females with Primary Infertility

Author: Ali H. Ad'hiah
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2008 Volume: 50 Issue: 4 Pages: 475-479
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The study was conducted to investigate the association between hyperprolactinaemia and markers of human leukocyte antigen (HLA) system in a sample of Iraqi infertile females, together with the profile anti-nuclear antibodies (ANA). Objectives: One hundred and seventy five female patients (age range: 20 -40 years) were recruited in this study. They were attending the Institute for Embryo Research and Infertility Treatment (Al-Nahrain University) during the period January 2005 - September 2006. Results:After clinical and laboratry evaluations, it was found that 100 patients were hyperprolactinaemic, whereas the other 75 patients were euprolactinaemic, therefore, they were considered as a control group. Based on serum level of prolactin (22-29, 30-39 and ≥ 40 ng/ml), the total hyperprolactinaemic patients were divided into three groups; I (35 patients), II (40 patients) and III (25 patients), respectively. The HLA antigens showed significant variations between patients (total and groups) and controls. In total patients, B8 (25.0 vs. 9.3%), DR3 (48.0 vs. 17.3%) and DR4 (39.0 vs. 13.3%) showed significant increased frequencies, while B35 showed a significant decreased frequency (7.0 vs. 24%). The latter decrease was also observed (5.7 vs. 24.0%) in group I of patients, which also showed a significant increased frequency of DR3 (54.3 vs. 17.3%). In groups II and III of patients, only DR3 (45.0 and 56.0, respectively vs. 17.3%) and DR4 (37.5 and 56.0, respectively vs. 13.3%) showed significant increased frequencies. Autoantibody evaluation by ANA test revealed that 22% of the total patients was positive, while all control subjects were negative, and such positivity paralleled the increased level of serum prolactin.


Article
Allelic Distribution of Human Leukocyte Antigen in Patients with Pulmonary Tuberculosis in Baghdad City

Author: Falah Salim Manhal
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 3 Pages: 253-258
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Host genetic factors such as human leukocyte antigens (HLA) and non-HLA genes that are associated with the susceptibility to tuberculosis (TB) will serve as genetic markers to predispose or predetermine the development of the disease.OBJECTIVE:The aim of this study is to analyze the association between particular HLA-typing class I and the incidence of pulmonary tuberculosis in Baghdad city.PATIENTS & METHODS:Blood samples were collected from one hundred patients; 50 samples from patients with pulmonary tuberculosis referred to the Chest and Respiratory Diseases Institute in Baghdad city and 50 samples from apparently healthy individuals. All samples were submitted to the lymphocytotoxicity test (NIH) and examined in Major Histocompatibility Laboratory in AL- Karama Hospital in Baghdad city.RESULT:It was found that HLA (15) expression was significantly higher in recently infected patients with tuberculosis than in the controls (p<0.01) and HLA-A (33) was significantly lower in those patients than the controls (p<0.05). HLA-A (1) was high significantly lower in historical TB patients than the controls (p<0.01). HLA-B (17) was significantly higher in recently infected patients with TB than the controls (p<0.05), HLA-B(35) was high significantly lower in historical TB patients than the controls (P<0.01). This study concluded that frequencies of HLA-A (15), HLA-B (17), in recently diagnosed pulmonary TB patients were significantly increased compared with those in the control group.CONCLUSION:The development of pulmonary tuberculosis infection is partly controlled by genetic factors. Sophisticated techniques such as (PCR) are needed for more assurance to verify this association. Further studies are required to investigate a possible relation between HLA-typing class II and the incidence of pulmonary tuberculosis in Iraq.


Article
THE RELATIONSHIP BETWEEN HLA-DRB1*0103 AND HLA-DQA1*0201 GENOTYPES AND SPECIFIC IGE RESPONSES TO UNRELATED ALLERGENS IN ATOPIC PATIENTS
العلاقة بين النمطين الجينيينHLA-DRB1*0103 and HLA- DQA1*0201واستجابةIgE الخاصة بالمستارجاتعديمة الصلة في مرضى الحساسية

Author: Falih H.Mezban*Fawziah A. Abdulla فالح حمود مزبان* فوزيه علي عبدالله**
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2014 Volume: 13 Issue: 1 Pages: 260-273
Publisher: Basrah University جامعة البصرة

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Abstract

Because of the pivotal role of the human leukocyte antigen (HLA) class II molecules in regulating the immune response and their extensive polymorphism, it is not surprising that particular HLA class II alleles have been implicated in susceptibility to allergic diseases and in restriction of the IgE responses to a variety of allergens. We investigated the relationship between HLA-DRB1*0103 and HLA- DQA1*0201 genotypes and allergies to amoxicillin(AX) and love bird (LB) and explored their restriction of IgE responses to these allergens.Enzyme Linked Immunosorbent Assay test (ELISA) was used to examine the specific IgE antibodies in the sera of 175 subjects including 145 atopic patients and 30 healthy individuals without any allergic reaction. All of them were chosen for HLA genotyping by polymerase chain reaction . Compared with seropositive all control and seronegative individuals showed negative PCR HLA genotyping results.The higher overall rate (39.4%) of PCR positivity was observed inseropositive patients with positive PCR HLA-DQA1*0201 genotyping results .Also PCR HLA-DQA1*0201 genotyping revealed the higher rates of the PCR positivity in the males and females of the second age group in comparison with those of the first age groups but without significant difference (P > 0.05). The same result was also observed in positive PCR result for the patients with HLA-DRB1*0103 and those with both HLA -DRB1*0103 and HLA-DQA1*0201 as the non-significant higher PCR positive rates was observed in the males and females of the 2ndage groups.Also the females of the second age group showed the higher PCR positivity rates (100%,50% and30%)for HLA-DQA1*0201 and HLA-DRB1*0103) and both respectivelyELISA results revealed that the overall rate of seropositivity in tested individuals to amoxicillin(AX) or love bird dropping extract (LBDE) allergens was 54.9% .The females were not significantly different (P ≥ 0.05) from males and they showed the higher rate of seropositivity against AX(57.9%)and LBDE(58.9%)allergens.In contrast there was no significant effect(P > 0.05)for the age on the seropositivity against LBDE and the patients of the 1st age group showed higher rate (55.9%) of seropositivity in comparison with rate (43.7%) of 2ndage group patients

لبعض الاليل الجينات ا لخاصة بمستضدات كريات الدم البيضاء من الصنف الثاني في الانسان علاقه بامراض الحساسية وتحديد استجابة الضد IgE لانواع مختلفه من المستارجات. وذلك بسبب دورها الحيوى في تنظبم الاستجابه المناعية والتغير المفرط في اشكالها وقد تم التحقق من العلاقة بين النمطين الجينيينHLA-DRB1*0103 and HLA- DQA1*0201والحساسية للاموكسيسيلين وروث طيور الحب و التحري عن دورها في تحديد استجابة IgE لهذه المستارجات .استخدم اختبار ELISA للكشف عن الضد IgE في مصول 175 شخص تتضمن145 مريض بالحساسية و 30شخص سليم جميع هؤلاء الاشخاص تم اختيارهم للتنميط الجيني بواسطة تفاعل سلسلة البلمره PCR.بالمقارنة مع الاشخاص اللذين اظهرو نتائج ايجابية مصلية فان جميع اشخاص السيطره وذوي النتائج المصلية السلبية اظهرو نتائج سلبية في التنميط الجيني بواسطة تفاعل سلسلةالبلمره ولوحظ النمط الجينيHLA-DQA1*0201 باعلى نسبة ايجابية (39.4%)للتنميط الجيني بختبارPCR في المرضى ذوي النتائج المصلية الايجابية كذلك ظهر هذا النمط الجيني باعلى نسبه ايجابية لاختبارPCR في ذكور واناث مرضى الفئه العمرية الثانية بالمقارنة مع مرضى الفئةالعمرية الاولى ولكن بدون فارق احصائي معنوى(P > 0.05).لوحظت نفس النتائج الايجابية لاختبار PCRلمرضىى الفئة العمرية الثانية ذكورا واناثا فيما يخص النمط الجينيHLA-DRB1*0103 كنمط جيني منفردا او مشتركا معHLA-DQA1*0201وبدون فرق احصائي معنوى 0.05) (P > وكذلك اظهرت الاناث من الفئة العمرية الثانية نسب PCRايجابية عالية (100%و50%و30%) وعلى التوالي لكل من النمطين الجينيين DQA1*0201 وHLA-DRB1*0103 وللاثنين معا.اظهرت نتائج ELISA بان اعلى نسبة كلية للايجابية المصلية للاشخاص المفحوصين بمستضدات الاموكسيسيلين وبراز طيور الحب كانت بمقدار54.9%.لم تختلف الاناث عن الذكور احصائيا(P ≥ 0.05) وفي الاشخاص المفحوصين كانت نسبة الايجابية المصلية ضد الاموكسيسيلين(57.9%)ومستارجاات براز طيور الحب(58.9%). لا يوجد تاثير محسوس احصائيا(P > 0.05)للعمرعلى الايجابية المصلية ضد مستارجات براز طير الحيوان لمرضى الفئه العمرية الاولى اظهرو اعلى نسب (55.9%)للايجابية المصلية بالمقارنة مع نسب مرضى الفئه العمرية الثانية (43.7%).


Article
HLA prevalence in Iraqi patients with ischemic heart disease

Authors: Eman Sh. AL-Obeidy --- Basil N. AL-Dileamy
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2010 Volume: 52 Issue: 2 Pages: 182-184
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The etiology of ischemic heart disease (IHD) is believed to have an immunological component. Association with human leukocyte antigens (HLAs) has been previously reported, particularly with DR6.Patients and methods: 75 cardiac patients were admitted to the coronary care unit, Baghdad Teaching Hospital over the period October 2008-May 2009 with the clinical diagnosis of acute coronary syndrome and STEMI myocardial infarction their ages range was (25-82) years the number of male was (55) (73.3%) and female was (20) (26.7%). All cases have routine ECG, cardiac marker’s measurements, routine haematological, Biochemical test and 2mls of blood reserved for HLA study.
Results: It was found that HLA-DR1 (8%) (P 0.001) has significant risk factor in the development of ischemic heart disease while HLA B (62%) (P 0.0009) has a protection factor in ischemic heart disease.
Conclusions: Our result suggests that strong relation between the incidence of acute ischemic episode (acute coronary syndrome and STEMS myocardial infarction and HLA-DR1) which mean that there may be predisposing genetic factor for the development ischemic heart disease.


Article
The possible Association of HLA Class II with Bladder Cancer in Iraqi Patients

Author: Shatha M.J.Al-Khateeb
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: PISSN: 00419419 / EISSN: 24108057 Year: 2007 Volume: 49 Issue: 4 Pages: 434-437
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: - Genetic Factors have a major role in the development of bladder cancer. Objectives: - This study was carried out to shed a light on the possible association of HLA class II antigens and BC patients and to correlate this finding with the family history. Patients and Methodes :- Lymphocytotxicity assay had been used to assess HLA- typing of 65 BC patients and 50 healthy controls. Results:- comparison between BC patients and healthy controls showed several antigens deviations in their frequencies. HLA-DR1, HLA-DQ1 and HLA-DQ3 antigens were observed with increased frequencies in patients group with significant differences (P=0.000, 0.000 and 0.017 respectively). Moreover there was decrease frequency of HLA-DR7 in patients group (P=0.010). Stastical analysis showed non significant correlation of the specific HLA –Ags with family history. Conclusions: - This finding demonstrated that HLA-DR1, DR7, DQ1 and DQ3 might play a role in BC susceptibility. • Department of clinical biochemistry. Collage of Medicine Al-Mustansyriah University • Department of Microbiology. Collage of Medicine. Baghdad University • Ministry of Health.


Article
HLA-DRB ALLELS, IL-10 AND VITAMIN D LEVEL: POTENTIAL IMPACT ON MULTIPLE SCLEROSIS

Authors: Ahmed A.H. Abbas احمد عبد الحسن عباس --- Alaa H. Khaliel علاء حسن خليل --- Nawfal M. Shaheed نوفل ماضي شهيد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 159-165
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Multiple sclerosis (MS) is a chronic inflammatory autoimmune disease with unknown etiology. Variation in the HLA-DRB1 gene is the potent genetic risk factor for promoting MS. Other agents relate with an increased risk of developing MS include the cytokines levels such as interleukin-10 (IL-10) and the vitamin D (vit. D) deficiency.Objectives:To study a possible role of HLA-DRB1, vit. D deficiency as a risk factor for MS development and to estimate the level of IL-10 in the serum of MS patients and its role in disease initiation or progression.Methods:Sixty MS patients, of them 30 were newly diagnosed with an age range between 13 and 58 years were included in this study, in addition to thirty healthy volunteers their gender and age matched with patients group serve as a control group. Blood samples collected to assess serum levels of vit. D and IL-10 by Enzyme-Linked Immunosorbant Assay (ELISA) and for DNA extraction, which used in the HLA-DRB1 2 digit genotyping.Results :The HLA-DRB1 genotyping revealed that the HLA-DRB1*15 frequency was higher but statistically insignificant in the MS patients as compared with healthy control group. IL-10 level was significantly lower in MS patients on treatment than the control group. On the other hand, vit. D in the newly diagnosed MS patients was significantly different from the control group (higher in the control group), but there was no variance with MS patients on treatment, the level of vit. D in the studied groups was less than global value.Conclusion:In MS patients the frequency of HLA-DRB1*15 was higher than control group but the difference was not significant. In addition, the level of IL-10 and vit. D may have a role in the development of MS.Keywords:Multiple sclerosis, human leukocyte antigen, IL-10


Article
Human Leukocyte Antigens Assosiation with Systemic Lupus Arythematosus In Iraqi Patients

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Background: The etiology of Systemic lupus erythematosus seems to be multifactorial including environmental as well as genetic factors. The genetic predisposition was supported by the occurrence of Systemic lupus erythematosus in more than one member of a family as well as in identical twins.Aim of the study: To determine the human leukocyte antigen typing class I (A and B) in patients with Systemic Lupus Erythematousus disease. Methods: Patients group consisted of 44 Iraqi Arab Muslims patients with Systemic lupus erythematosus disease who presented to Baghdad Medical City from January 2010 to January 2012 from Baghdad Province. The second control group consisted from 80 Iraqi Arab Muslims volunteers from hospital employees and their relatives. Human leukocyte antigen typing done for them using serological method by microlymphocytotoxicity test.Results: A total of 44 patients with Systemic lupus erythematosus were studied. Patients' age ranged from 6 to 65 years with a mean of 29.61 ± 12.78. The other control group, their ages ranged from 16 to 55 years with a mean of 31.35 ± 10.02 . Females were more than males in both groups. Human leukocyte antigen typing of Systemic lupus erythematosus patients showed A2( 16, 36.36, 0.20), A3( 14, 31.82, 0.17), B4(10, 22.73, 0.12) , B12 (10, 22.73, 0.12) and B21(10, 22.73, 0.12) were the highest absolute numbers, phenotype frequencies and genotype frequencies respectively. There was a significant difference in between SLE patients and control group in the following alleles (A1, A28, A36, A43,B 6, B12, B14, B15, B16, B35, B42, B53).Conclusions: Genetic factors do have a role in the development and expression of Systemic lupus erythematosus . human leukocyte antigen -A28 , A36, B12 and B21 had an association with Systemic lupus erythematosus Iraqi patients.Key words: Human Leukocyte Antigens, Systemic Lupus Erythematosus , Tissue typing


Article
Gene frequency and haplotype analysis of HLA class I in patients with simple renal cysts

Author: Aroub A.R Al-kaisi M.B.Ch.B, F.I.C.M.S*, Khalida M. Mousawy M.B.Ch.B, Ph.D **, Usama N. Rifat M.B.Ch.B, F.R.C.S ***
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2015 Volume: 11 Issue: 1 Pages: 58-61
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: The study of human leukocytes (HLA) alleles, and haplotype frequencies within populations provide an important source of information for anthropological investigation, organ and hematopoietic stem cell transplantation as well as disease association, certain diseases showed association with specific alleles specially those of known or suspected hereditary origin or immunological basis, whether simple renal cyst is congenital or acquired is still unclear and need to be investigated.Objectives: To study the genetic aspect of simple renal cysts by detecting the gene frequency and the haplotype of HLA class I of patients with simple renal cysts, and to find the presence of these cysts in other family members.Method: Thirty patients with simple renal cysts who were attending the outpatient clinic of urosurgery in the medical city were tested for HLA class I antigen using the microlymphocytotoxicity technique, in the period from February to June 2004 compared to 50 unrelated apparently healthy individuals. Gene frequency were calculated using square root formula (g=1-√1-f), full history were taken including the family history.Results: Certain gene frequencies were higher in the patients group than in the controls, yet not reached to a statistical significant level. No haplotype association with simple renal cysts was detected in this study; family history was detected in two patients which were proved by ultrasound examination.Conclusion: Increasing the sample size may contribute to best results regarding gene frequency, haplotype and family study.Key words: Gene frequency, Haplotype, Human Leukocyte Antigens.

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