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Article
MEASUREMENT OF MALONDIALDEHYDE AND THIOL LEVEL IN IRAQI PATIENTS WITH GASTRO DUODENAL DISEASES
قياس مستوى الثايول وثنائى الالديهايد عند المرضى العراقيين المصابين بإلتهاب وقرح المعدة

Author: نوح عبد القادر محمود
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2009 Volume: 8 Issue: 1 Pages: 465-472
Publisher: Baghdad University جامعة بغداد

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Abstract

It has been suggested that the free radicals are closely related with peptic ulcer disease and gastritis. Although many studies have been undertaken to clarify the role of oxygen-derived free radicals, most of them were carried out animal models. The aims of this study were to assess the reactive oxygen species activity and the damage in Helicobacter pylori infected gastric mucosa in humans. In a total 50 patients there were 35cases of peptic ulcer, 15 cases of gastritis and12 control subjects. Measurement of plasma malondialdehyde concentrations, which is the end-product of lipid peroxidation, was used to assess oxidative damage to membranes in patients with peptic ulcer and gastritis. Plasma glutathione concentrations were also measured in order to study whether reactive oxygen species generation affects levels of the antioxidant peptide. Results showed that plasma levels of glutathione were significantly reduced (p <0.001) and malondialdehyde was higher (p<0.001) in patients with peptic ulcer compared to controls. In patients with gastritis, glutathione was also lower (p<0.001) and malondialdehyde higher (p<0.01). The study concluded that depletion of plasma glutathione in cases with H. pylori positive peptic ulcer and gastritis may be caused by accumulation of free radicals that can initiate membrane damage by lipid peroxidation that increased plasma malondialdehyde concentration

تم قياس مستوى Malondialdehydeفي هده الدراسة وهو الناتج النهائى لأكسدة الدهون فى جدار الخلية ودراسة مستوى الثايول Thiol فى مصل خمسين مريضاً، 35 مريضاً مصاب بقرح المعدة و15 مريضاً مصاب بإلتهاب المعدة المزمن.تم أخذ 12 عينه طبيعيه لتكون مجموعه سيطره إذ تم دراسه مستوى الدهون المؤكسده والتي يعد المالون الثنائي الالديهايد الناتج النهائى لهذه العمليه وأيضاً تم دراسه مستوى الثايول المصدر الرئيس للانزيمات المضاده للأكسده.وجد ان تركيز MDA كان قد ارتفع بشكل ملحوظ (( P< 0.001 مع انخفاظ واضح في نسبة الثايول (( P<0.001 ويمكن أن يعلل دلك من خلال زيادة وتراكم الجدور الحرة أدى إلى زيادة تأكسد الدهون في جدار الخلية والتى هي فى معضمها دهون مفسفرة phospholipids مما أدى إلى زيادة نسبة MDA مع نقص واضح فى تركيز الثايول. أستنتج من الدراسة ان نقصان مستوى الثايول وارتفاع مستوى الدهون المؤكسده له علاقه مع الإصابه ببكتريا الملويات البوابيةHelicobacter pylori والتي تؤدي إلى زياده تراكم الجذور الحرة.

Keywords

Malondialdehyde --- Thiol


Article
Relationship of Nitric Oxide and Malondialdehyde to Miscarriage
علاقة تركيز اوكسيد النتريك وتركيز المالوندايالدهايد مع الاجهاض

Authors: Asmaa K. Gatea --- Balsam G Hassan --- Ahmed M Issa
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2012 Volume: 9 Issue: 4 Pages: 777-785
Publisher: Babylon University جامعة بابل

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Abstract

AbstractThe present study was conducted to verify the oxidative stress status in miscarriage .To achieve this aim, 70 women with miscarriage (patient group) , and 25 healthy non pregnant (control group) were subjected to the study. The statistical analysis ( student's t-test) showed a significant (p<0.001) increase of nitric oxide (NO) and malondialdehyde (MDA) levels , in women with miscarriage when compared with those of the control group .The linear regression analysis demonstrated a significant positive correlation for both MDA values(r =0.47, p<0.01) and NO values(r =0.45, p<0.01) with gestational ages .The same analysis indicated a significant positive correlation for MDA (r =0.73 , p<0.01) and (r =0.71 , p<0.01) for NO levels with the number of previous miscarriages was observed . The results indicate that oxidative stress may lead to miscarriage.Key words: nitric oxide, malondialdehyde, miscarriage.

الخلاصة تم تصميم الدراسة الحالية للتحقق من تغيرات الإجهاد التأكسدي عند مريضات الاجهاض التلقائي . شملت الدراسة 70 مريضة و 25 امراءة غير حامل سوية بوصفهم مجموعة سيطرة . تم تقدير مستويات اوكسيد النتريك و مالون ثنائي الألديهايد في أمصال المرضى ومجموعة السيطرة . وجد زيادة ملحوظة p<0.001)) في مستوى اوكسيد النتريك و مالون ثنائي الألديهايد لدى مريضات الاجهاض عند مقارنتها مع مثيلاتها في مجموعة السيطرة. اظهرتحليـل الانحدار الخطي ارتبـاطاً معنويا موجباً لكل من مستوى مالون ثنائي الألديهايد ( r=0.47 , p<0.01) ومستوى اوكسي النتريك( r=0.45 , p<0.01) لدى المريضات نسبةً إلى التقدم في مدة الحمل. . كما أظهرت النتائج وجود ارتباط معنوي موجب في مستوى كل من مالون ثنائي الألديهايد ((r=o.73, p<0.0 ومستوى اوكسيد النتريك( r=0.71, p<0.01) مع عدد الاجهاضات السابقة . توضح النتائج ان الاجهاد التاكسدي يؤدي الى الاجهاض.


Article
Trace elements and oxidative stress markers in saliva of subjects with amalgam fillings

Authors: Huda Sh. Ahmed هدى احمد --- Taghreed F. Zaidan تغريد زيدان --- Ali Yakub علي يعقوب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 63-66
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Amalgam is the most frequently used restorative material for dental treatment. It is mainly used inposterior teeth, usually on occlusal surfaces as an economical, long lasting and durable filling material and representthe main source of exposure to mercury and other toxic metals (copper, tin, silver, etc.).This study designed to measure oxidative stress marker malondialdehyde (MDA) and anti-oxidants (uric acid andglutathione) concentrations in saliva of subjects with amalgam fillings. And measure trace elements (copper, zinc)concentrations in saliva of subjects with amalgam fillings.Subjects,materials and methods: Fifty subjects were participated in this study, they were between the age of (20-50)years with amalgam fillings (cases group) and fifty one subjects with no amalgam fillings (control group), they weregender and age matched to that of subjects with amalgam fillings. Informed consent and ethical approval wasobtained. Each subject fill a case sheet questionnaire then examined by using sterile dental mirror and sterile dentalprobe to determine any oral manifestations and to calculate the number of amalgam filled teeth and the number offilled surfaces.Results: The results obtained from this study showed that Oxidative stress marker (MDA) were increased whileantioxidants (glutathione, uric acid) were decreased in saliva of subjects with amalgam fillings. Trace elements(copper, zinc) were higher in saliva of subjects with amalgam fillings; salivary copper was significantly higher insubjects with > 10 amalgam filled teeth. Salivary total glutathione was significantly correlated (negative correlation)with the number of filled teeth. Salivary copper was significantly correlated (positive correlation) with the number offilled teeth and filled surfaces.Conclusion: This study revealed that amalgam fillings associated with increase in oxidative stress marker(MDA) anddecrease in antioxidants (glutathione, uric acid).Trace elements (copper, zinc) increased in saliva of subjects withamalgam fillings.


Article
Thyroid Disorders and the Level of Malondialdehyde

Authors: Eham A. Ali * PhD PhD --- Yildiz H. Tahseen** --- Hedef D. El-Yassin** PhD هدف ظافر الياسين
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 1 Pages: 67-70
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Free radicals have been implicated in many pathological processes, includingischemia, inflammation, and malignancy. The free radicals may affect tissues damage by lipidperoxidation which generate malondialdehyde (MDA) as a by byproduct of the reaction. Theobjective of this study is to examine the dynamics of lipid peroxidation in patients withthyroid disorders using the measurement of malondialdehyde level as a marker for the degree ofthyroid disorder.Methods: Two hundred and forty five subjects were enrolled in this study. Hundred andninety five were patients with different thyroid disorders (88 yperthyroidism, 63hypothyroidism and 44 thyroid carcinoma) the remaining 50 subjects were healthy oneswithout any apparent functional disorders served as a control. Malondialdehyde (MDA) levelwas estimated in the sera of all subjects.Results: A significant increase in the level of serum MDA concentration was observed inpatients with all types of thyroid disorders as compared with the control groups.Conclusion: The findings obtained showed a high


Article
Estimation of Salivary Resistin, malondialdehyde and Lipid Profile levels in patients with Diabetes Mellitus
تقدير مستويات رزيستين ، المالون ثنائي الديهايد ومستوى الدهون اللعابية في المرضى الذين يعانون من داء السكري

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The present study is designed to verify the changes of resistin, malondialdehyde (MDA), glucose and lipid profile in type2 diabetes mellitus. This study was carried out during the period from November 2013 till January 2014, on 96 subjects: 57 diabetic patients (11 males and 46 females, aged between 33 and 60 years, with a mean age of 40.32) and 39 healthy controls (24 males and 15 females, aged between 40 and 59 years, with a mean age of 47.43), who attended :outpatient, Health care center, and Emergency Department in Tikrit Teaching Hospital.The results showed a significant increase in the salivary glucose, total cholesterol (TC), triglycerides (TG) resistin, and MDA level in diabetic patients compared to controls, while significant decrease in salivary HDL in diabetic patients compared to controls.

تم تصميم هذه الدراسة للتحقق من التغييرات من رزيسستين ، المالون ثنائي الديهايد ، الجلوكوز ومستوى الدهون في مرض السكري. اجريت الدراسة خلال الفترة من نوفمبر 2013 حتى يناير عام 2014 على 96 شخص : 57 منهم مرضى السكري (11 من الذكور و46 من الاناث، تراوحت اعمارهم بين 33و 60 سنة مع متوسط عمر 40,32) و 39 من الاصحاء (24 منهم من الذكور و 15 من الاناث، تروحت اعمارهم بين 40 و59 عاما مع متوسط عمر 47,43) ، الذين راجعوا :االعيادات الخارجية، مركز الرعاية الصحية، وقسم الطوارئ في مستشفى تكريت التعليمية. اظهرت النتائج زيادة ملحوظة في نسبة جلوكوز اللعاب ، TC, TG رزيستين ومستوى ، المالون ثنائي الديهاي في مرضى السكري مقارنة مع الاشخاص العاديين في حين اظهر انخفاض ملحوظ فيHDL اللعاب في مرضى السكري مقارنة مع الاشخاص الطبيعيين.


Article
THE EFFECT OF ZINC AND COPPER DEFICIENCY ON HEMATOLOGICAL PARAMETERS, OXIDATIVE STRESS AND ANTIOXIDANTS LEVELS IN THE SHEEP.

Author: Hussein Ali Naji.
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2017 Volume: 16 Issue: 2 Pages: 344-355
Publisher: Basrah University جامعة البصرة

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Abstract

The objective of this study was to determine the levels of Zinc (Zn) andcopper (Cu) concentration, as well as the clinical signs that may be occur due to theZinc and copper deficiency, in addition to the effect the two trace elements levels onthe hematological levels, oxidative stress Malondialdehyde (MDA) and antioxidantlevels glutathione and Catalase (CAT). This study carried out in Basra province, thestudy conducted on (75) sheep (male and female). About 60 sheep clinically havefrom zinc and copper deficiency (hypozincemia) and 15 clinically apparently healthyserved as a control group. The clinical signs occur due to the tow trace elementsdeficiency include: loss of appetite, depression, pica, repeatedly bites off the wool ofother sheep, loss of the wool, loss of hair around the eyes, ear and nose, alopecia,parakeratosis, stiff in gait and some animals suffering from diarrhea, pale of themucous membrane, decrease the rumenal contraction, and significant (P<0.05)increase the heart and respiratory rate with normal body temperature. Also the resultsshow significant increase (P<0.05) of MDA concentration, in addition to significantdecrease (P<0.05) in glutathione and CAT levels due to significant decrease (P<0.05)in both Zn and Cu concentration levels, this point the Zn and Cu play important rolein prevent damage occur due to the oxidative stress via decrease the free radicals, alsoit has role in antioxidant synthesis.

Keywords

Zinc --- Malondialdehyde --- Sheep


Article
Effects of menopause on serum oxidant status and lipid profile in Mosul city

Author: Bassam N. Aziz بسام
Journal: Iraqi Journal of Pharmacy المجلة العراقية للصيدلة ISSN: 16802594 Year: 2010 Volume: 9 & 10 Issue: 1 Pages: 1-8
Publisher: Mosul University جامعة الموصل

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Abstract

Objectives: To study the effect of menopause associated with estrogen deficiency on lipid peroxidation products, such as malondialdehyde (MDA) with evaluation of some antioxidants like, glutathione (GSH) and its relation to lipoprotein levels in women living in Mosul City.Design: Case-control study.Setting: The study was carried out in Al-Salam Teaching Hospital in Mosul City, during the period from January 2008 to April 2008.Patients and Methods: A total of 27 women aged 20–45 years were reported to be premenopausal and 42 women aged 45–60 years were recorded to be postmenopausal. Blood samples were collected for both groups. The assessments of serum malondialdehyde (MDA), glutathione (GSH), estrogen, arylesterase, calcium, total cholesterol, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) were done.Results: There were significant increase in MDA level in women after menopause in comparison with premenopausal age. On the other hand, GSH, estrogen, arylesterase, and calcium levels were significantly decreased. In respect to lipids, total cholesterol, TG, and LDL-c, were significantly increased in opposite to HDL-c, in which was decreased significantly in postmenopausal women in relation to premenopausal subjects.Conclusion: The increase of MDA and the decrease of antioxidants concentrations like gluthathione, estrogen and HDL-c in postmenopausal women could contribute to acceleration of the cellular oxidative damage.

أهداف البحث: دراسة تأثير سن اليأس المصاحبة لانخفاض الاستروجين في نواتج البيروكسدة المتمثلة بالمالونديالديهايد مع قياس بعض مضادات الأكسدة كالكلوتاثايون وعلاقتها بمستويات البروتينات الدهنية في النساء في مدينة الموصل.التصميم: دراسة عينية مقارنة.مكان اجراء البحث والاطار الزمني: نفذت الدراسة في مستشفى السلام التعليمي بمدينة الموصل, خلال الفترة من كانون الثاني 2008 ولغاية نيسان 2008.المرضى وطرق العمل: تضمنت هذه الدراسة 27 امرأة قبل سن اليأس تراوحت أعمارهم من 20-25 سنة , و 42 امرأة بعد سن اليأس تراوحت أعمارهم من 45-60 سنة. أخذت عينات الدم. تم في المجموعتين قياس كل من مستويات مصل دم المالونديالديهايد والكلوتاثايون وهرمون الأستروجين والأريل أستريز والكالسيوم وكمية الكوليستيرول الكلي والشحوم الثلاثية والبروتين الدهني واطئ الكثافة والبروتين الدهني عالي الكثافة.النتائج: أظهرت نتائج هذه الدراسة وجود زيادة معنوية في مستوى المالوندايالديهايد في النساء بعد سن اليأس مقارنة مع تلك المجموعة قبل سن اليأس. من ناحية أخرى, فقد انخفضت معنويا مستويات كل من الكلوتاثايون وهرمون الأستروجين والأريل أستريز والكالسيوم. وفيما يتعلق بالدهون فقد ازدادت معنويا مستويات كل من الكوليستيرول الكلي والشحوم الثلاثية والبروتين الدهني واطئ الكثافة وانخفض البروتين الدهني عالي الكثافة في النساء بعد سن اليأس قياسا للأفراد قبل سن اليأس.الاستنتاج: إن زيادة المالونديالديهايد وانخفاض مستويات مضادات الأكسدة كالكلوتاثايون والأستروجين والبروتين الدهني عالي الكثافة في النساء بعد سن اليأس يمكن أن يساهم في تعجيل التخريب الخلوي التأكسدي.


Article
Effect of Some Environmental Conditions on the Oxidative Stress and Transition Metals Status in Iraqi Subjects

Authors: Muhanad Mohammed Nori --- Saad Abdul-Rehman Hussain --- Nada Khadum Al- Tae'e --- Hameed Mahmood Majeed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 2 Pages: 147-151
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: It has long been recognized that exposure to various metals is highly toxic, producing a wide variety of illnesses, including cancer. The role of these metals in free radical formation and initiation of lipid peroxidation was extensively studied; where the free form of iron or copper can effectively be involved in a free radical generation. OBJECTIVES: To evaluate the effects of the some environmental conditions on the levels of the oxidative stress markers, malondialdehyde (MDA) and glutathione (GSH), and some of the transition metals, iron, copper and zinc, in he serum of Iraqi subjects who exposed to different environmental conditions. METHODS: Blood samples were taken from 137 healthy male adults who work in different types of jobs (25 farmers, 25 workers in gasoline, 25 workers in liquid propane gas, 25 workers in casting, 25 workers in paints and 12 workers in plastic and rubber materials). The serum levels of MDA, GSH, iron copper and zinc were estimated and compared in different groups. RESULTS: The result showed that exposure of healthy subjects to different occupational hazards produces significantly different changes in the oxidative stress markers as revealed by excessive production of the lipid peroxidation end product (MDA) and depletion of the soluble antioxidant (GSH); and impairment of the trace element status (copper and zinc). CONCLUSION: The extracellular defense mechanisms were severely affected by the insults of oxidative stress processes due to environmental pollution in some work places in Iraq.


Article
Evaluation of Superoxide Dismutase and Malondialdehyde in Asthmatic Patients in Al-Hilla Province
تقدير انزيم السوبراوكسيد دسميوتيز وتركيز المالوندايالديهايد لدى مرضى الربو في مدينة الحلة

Authors: Ahmed J Mohammed --- Ahmed M Issa
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2012 Volume: 9 Issue: 4 Pages: 806-814
Publisher: Babylon University جامعة بابل

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The present study was conducted to explore the oxidative stress levels in asthma. To achieve this aim, 87 asthmatic patients, and 46 healthy individuals were enrolled in this study. The samples were obtained from Babylon Asthma and Allergy center in Hilla city. The laboratory work was carried out in the research-lab of the departments of biochemistry, college of medicine, Kufa University, Iraq. Superoxide dismutase activity and malondialdehyde concentrations were determined spectrophotometrically. The used statistical analysis ( student`s t-test) showed a highly significant (p<0.001) decrease in superoxide dismutase and a highlysignificant (p<0.001) increase in malondialdehyde concentrations in asthmatic patients when compared with healthy individuals. The linear regression analysis demonstrated a significant negative correlation for superoxide dismutase (r =-0.63, p<0.01), and a significant (r =0.53, p<0.01) positive correlation for malondialdehyde concentration with age of asthmatic patients. The statistical analysis indicated a significant negative correlation for superoxide dismutase (r = -0.63, p<0.01) and a significant (r =0.44, p<0.01) positive correlation for malondialdehyde concentration with the duration of asthma. Patients from rural areas showed a significant (p<0.01) decrease of superoxide dismutase and a significant (p<0.01) increase of malondialdehyde concentrations when compared to those of urban residency. The results indicated that asthma is highly associated with oxidative stress. It may evoke the addition of antioxidant to the treatment of asthma,And those who live in rural area should ingest a high amount of anti-oxidant to ensure the balance between the oxidant species and the well-done anti-oxidant in the cell.

أجريتْ الدراسة الحالية لاستكشاف مستوى إلاجهاد التاكسدي لدى مرضى الربو ولتحقيق هذا الهدفِ،تم سحب عينات الدم من 87 مريضا مصابون بالرَّبوِ و46 شخصا من الأصحاء. تم الحُصِول على العينات مِنْ مركزِ الربو و الحساسيةِ في بابل. اجري العمل ألمختبري في مختبرِ قسمِ الكيمياء الحياتيةِ- كليَّة الطبِّ- جامعة الكوفة- العراق.حيث تم تقدير الفعالية لإنزيم (superoxide dismutase) SODوالتركيز للمركب MDA ((malondialdehyde طيفيا. اجري التحليل الإحصائي باستعمال T-test(اختبار تي) الذي اظهر نقصانا معنويا (p <0.001) في مستوى لدى SODمرضى الربو مقارنة بالأصحاء وتبين ان هنالك زيادة معنوية عالية(p <0.001) في مستويات MDAلدى المرضى المصابون بالرَّبوِ عند مقَارنتهم مَع الأصحاء من نفس الفئة العمرية. اظهر تحليل الانحدار الخطي إرتباطا سلبيا معنويا (r =-0.63, p <0.01) بين العمر بالسنوات وقيم SOD وارتباطا ايجابيا معنويا (r =0.53, p <0.01) بين اعمار المرضى وتركيز MDA لديهم.كما أجريت تحاليل الانحدار الخطي الإحصائية بين متغيرات الإجهاد التاكسديSODوMDA و مدة المرض بالسنوات حيث كان الارتبط إيجابي وملحوظ (r =0.44, p <0.01) لقِيَمِ MDA المصلية مع مدة المرض وسلبيا معنويا ((r = -0.63, p <0.0 لقيم SODفعالية عند ربطها بمدة المرض.كما وتم تقصي مستويات الإجهاد التاكسدي لدى القرويين من المرضى الساكنين ْفي المناطق الريفية النائية حيث اظهرت نتائجهم وجود نقصان معنوي (p <0.01) في مستوى فعالية الإنزيم SOD وزيادة معنوية (p <0.01) في تركيز MDA عند مقَارنتها َمع تلك المستقاة مِنْ ذوي الإقامةِ الحضريةِ. لقد دلت النَتائِجُ على أنّ الربو يُرتَبطُ بإلاجهادِ التاكسدي إلى حدٍ كبير.مما قد يستدعي إضافة مضادات الأكسدة الى العلاج.وان على المرضى المقيمين في الأرياف الاهتمام بتناول كميات وافية من مضادات الاكسدة لضمان المحافظة على التوازن المنشود بين الاصناف المؤكسدة الضارة ومضادات الاكسدة محمودة الاثر.


Article
Effect of Oxidative Stress on Iraqi Rheumatoid Arthritis Patients

Authors: Weaam F. Hussain --- Wisam Kadhum H. Al-Hashemi
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 2 Pages: 28-31
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Oxidative stress is an imbalance between oxidants and antioxidants, play an important role in the development of Rheumatoid Arthritis. Rheumatoid Arthritis regard as a chronic, autoimmune disease. The aim of the study to assess levels of some oxidative stress biomarker in Rheumatoid Arthritis. This study involves 80 Rheumatoid arthritis patients and 30 healthy subjects as control groups. Analysis on serum samples was Malondialdehyde, Albumin, and total Bilirubin. The obtained data showed significantly increasing (P<0.01) in level of oxidant parameter Malondialdehyde and significantly decrease (P<0.01) in endogenous antioxidant albumin and total bilirubin in comparison with the control group which may be due to increased activity of immune system which involves reactive oxygen species in its action. The reactive oxygen species of originated from the immune system have the crucial role in oxidative stress situation in Rheumatoid arthritis

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