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Article
Imaging and Diagnosis of different kind of Viruses by using (FITC,Evans blue ,and RB ) by Fluorescence Microscope

Author: Entiedhar Jasim Khamis Al -Yasari
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 4 Pages: 768-775
Publisher: Babylon University جامعة بابل

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Abstract

The use of fluorescence microscopy for imaging biomedical specimens has been expanded into all fields of diagnosis for different kinds of viruses , three viruses were imaged and diagnosed by the fluorescence microscope at Al-Sadr teaching hospital at Kufa city . The human metapneumo viruse hMPV , the respiratory syncytial viruse RSV and the measles chicken emberyo fibroblast viruse have been imaged and diagnosed by the fluorescence microscope in Al-SADR Educational hospital at Kufa city . Three types of dyes were added to the specimen solutions separately ,Rhodamine B , Fluorescene and Evans blue in order to obtain high resolution and contrast images. The absorption and fluorination spectra of these dyes were measured by EUV-visible spectrophotometer and fluorospectrophotometer respectively .


Article
Studying of Reflected Light Optical Laser Microscope Images Using Image Processing Algorithm

Author: F.H. Rajab
Journal: Iraqi Journal of Applied Physics المجلة العراقية للفيزياء التطبيقية ISSN: 18132065 23091673 Year: 2013 Volume: 9 Issue: 1 Pages: 15-19
Publisher: iraqi society for alternative and renewable energy sources and techniques الجمعية العراقية لمصادر وتقنيات الطاقة البديلة والمستجدة

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Abstract

In this work, the mechanism and principle of optical laser microscope (OLM) which works on the principle of light reflection that used in many applications were presented. Image of silicon wafer that obtained from OLM was enhanced and analyzed using image processing algorithm. The used algorithm was designed using MATLAB 10.0.The output images from RLOLM were compared with images of ordinary microscope.


Article
Observations on the ultrastructure of a rat mammary gland treated with harmal and borage

Authors: Salim R. Hamoudi ** --- Shatha M. Al-Faisaly*** --- Wasan Al- Saidi * --- Malak A. Al- Yawer **Ph.D
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 1 Pages: 151-156
Publisher: Baghdad University جامعة بغداد

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Abstract

Background : It had been indentified by histological, histochemical and morphometrical studies that peganum harmala is a mammogenic herb and borage officinalis is a lactogenic one . To complete our investigation about these two herbs , we performed electron microscopical study . Materials and methods : Rats were grouped according to their physiological status into three groups . Each group was subdivided in to three subgroups : one control and two experimental . The two experimental group were treated daily; the 1st one with an aqueous extract of peganum harmala seeds and the 2nd with an aqueous extract of borage officinalis flowers . After two weeks of treatment , mammary glands were employed for electron microscopical study . Results : In virgin rats , the epithelial and myoepithelial cells were partially differentiated when harmal was given and completely differentiated when borage was given . In pregnant rats , harmal and borage optimize mammary parenchymal growth and induce lactation when these herbs were given. In lactating rats ,these herbs exhibited a picture similar to control lactating group but the budding of lipid droplets and the swelling of secretary vesicles were markedly increased . Conclusion: Both harmal and borage stimulate the release of prolactin and induce galactogenesis during pregnancy and promote it during lactation . Key Words : Mammary gland , Electron microscope , Harmal , Borage


Article
Comparative study of wettability of different lining, tissue conditioning and denture base materials (in-vitro study)

Authors: Mostafa S. M. Al-Shaikhli مصطفى الشيخلي --- Amir H.M. Khamas عامر خماس
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: special issue 1 Pages: 24-30
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Wettability of denture base and denture relining materials is one of the most important properties fordenture retention, because it provides a condition in which saliva will spread over the surfaces with ease. Contactangle has been highlighted as the most important parameter concerning wettability of materials. This angle ischaracteristic of the substances due to the surface tension of the liquid and the surface energy of the solidMaterials and methods: One hundred and twenty specimens were prepared in six major groups (light cure acrylic,hot cure acrylic, flexible acrylic, soft liner, hard liner and tissue conditioner) with dimension of (25mm X 25mm X4mm)each group consists from 20 specimens. The wettability was measured using digital microscope and the retentionforce was measured using specially designed equipment.Results: The results obtained in the present study showed that there is a highly significant difference on the contactangle between different materials, different type of fluids (distilled water and synthetic saliva). The contact anglevalue of light cure acrylic is smaller than heat-cure acrylic, tissue conditioner, hard liner, flexible acrylic and soft linerrespectively; it was greater for synthetic saliva than those for distilled water,Conclusion: In conclusion, Light cure acrylic had the best wettability followed by hot cure acrylic and tissueconditioner respectively. There is an effect of the viscosity on the contact angle, and the contact angle has aneffect on the retention force. When the contact angle value was small the surface retention was higher than whenthese angles were large


Article
Apoptotic Features in Normal Rabbit’s Adenohypophysis Cells (Electron Microscopic and Quantitative Study)
أشكال الذوي في خلايا النخامية الغدية للأرنب(دراسة بالمجهر الإلكتروني ودراسة كمية)

Author: Daad A. Hussein¹, Hussein A. M. Dauod, Kawkab S. Nejim
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2013 Volume: 12 Issue: 1 Pages: 51-57
Publisher: Baghdad University جامعة بغداد

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Abstract

The earliest notable signs of the apoptosis such as the chromatin mass condensation and gradually migration peripherally toward the nuclear envelope were recognized. The terminal end of the apoptotic pathway, represented with apoptotic bodies and membrane blebs or blisters appearances recognized too. In general, apoptotic cells were decreased with progressing animal age; however, there was only significant increase of the apoptotic cells between baby group and adult group in rabbit adenohypothysis with t-value 2.46 and p< 0.05.

لقد تم إستعمال المجهر الإلكتروني النافذ في الدراسة الحالية لغرض التعرف على الخصائص النوعية والمظرية (الفحص البايلوجي) لخلايا النخامية الغدية للأرنب كوسيلة مساعدة للتحري عن أشكال الذوي على صعيد النواة والخلية فضلاً عن التحليل الإحصائي للخلايا الذاوية طبقاً لعمر الحيوان. وتمت ملاحظة بعض العلامات المبكرة لآلية الذوي في الخلايا وهو تكاثف الكروماتين وهجرته بشكل تدريجي محيطياً بإتجاه الغلاف النووي. إن العلامات النهائية والختامية لمسار الذوي والمتمثلة بظهور الأجسام الذاوية وبثرات غشائية قد أشر أيضاً. وتمت ملاحظة نقصان في عدد الخلايا الذاوية مع تقدم عمر الحيوان, وعلى كل حال, كان هناك إرتفاع معنوي وحيد للخلايا الذاوية لمجموعة الرضع عند مقارنتها بمجموعة البالغين.


Article
Computer Aided Flank Wear Measurement in End Milling Cutting Tool

Authors: Ali Abbar Khleif --- Mostafa Adel Abdullah
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2016 Volume: 34 Issue: 5 Part (A) Engineering Pages: 959-972
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

Flank wear width is generally recognized as the key indicator for tool life.In the experiments of this study, nine tools made of HSS and stainless steel 316L as work piecewiththreespindlespeeds(550,930 and 1100)rpm and three redial depth of cut (1.5,2 and 2.25)mm were used. The cutting tool wear was measured using optical microscope and vision system based on a proposed algorithm.Maximum and minimum percentage errors in the flank wear width were (8.250% and 0.645 %) respectively. The numerical method used was by a multiple linear and polynomial regression model and developed a polynomial model, especially to predict the flank wear using MATLAB software.Maximum and minimum percentage errors werefound (14% and 0.322 %) respectively.


Article
Analytical Investigation of Charging Ability of Insulators under Electron Beam Irradiation inside SEM Chamber

Author: Hassan N. Al-Obaidi1, Ali S. Mahdi2
Journal: Almuthanna Journal of Pure Science (MJPS) مجلة المثنى للعلوم الصرفة ISSN: 22263284 Year: 2016 Volume: 3 Issue: 2
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

An analytical procedure has been carried out to measure the charge that may trapped in an insulator sample andrelated electrostatic surface potential in sense of mirror effect phenomenon. In fact, scanning electron microscope mirrormethod (SEMME), sometimes called electron mirror method (EME) and/or magnification factor method (MFM), has beenused to accomplished that purpose. However, this work has been carried out concerning the theoretical point of view, themirror plot curve has been adopted as an evaluation scale for the quality of the mirror image. Therefore, this procedure hadbeen used to investigates the experimental mirror plot curves for PMMA material with different accelerating potential andstudying the most important parameters that affects in these curves. Results have clearly shows that the radius of irradiatedarea play an important rule in the shape of mirror plot figure and then the quality of the image.


Article
Histological and fluorescent microscope studies for evaluation carbon accumulation in trachea and bronchi of birds in polluted area in Wasit province
دراسة نسيجية وباستخدام المجهرالمتالق لتقدير تراكم الكاربون في الرغامي والقصبة الهوائية للطيور في المناطق الملوثة من محافظة واسط

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Abstract

The aim of this study was to detect the accumulation of carbon and determination its appearance in different areas of groups of ducks. Using special dyes to detect deposits of carbon particles. Also, using AO/ EB stains to detect early and late (progress) apoptosis that occurred due to the precipitated of carbon in both areas (Al-ahdeb oil field and brick factories areas) but late apoptosis occurred in bronchi of brick factories area more than oil field area. The histological examination of trachea showed no any indicator of accumulation of carbon in three different areas, whereas in bronchi showed the presence of carbon in polluted areas (Al-ahdeb oil field and brick factories areas) in different amounts.

الهدف من هذه الدراسة هو الكشف عن تراكم الكاربون وتحديد مظهره في مناطق مختلفة لمجموعات من البط باستخدام اصباغ خاصة للكشف عن ترسب جزيئات الكاربون. وكذلك استخدام صبغات الاكردين البرتقالي/ الاثيديوم بروميد للكشف عن الموت المبرمج المبكر والمتأخر(المتقدم) الذي يحصل نتيجة لترسب جزيئات الكاربون في كلا المنطقتين الملوثة (منطقة حقل الاحدب النفطي ومنطقة معامل الطابوق) ولكن حصول الموت المبرمج المتأخر في منطقة معامل الطابوق يكون اكثر مما هو عليه في منطقة حقل الاحدب النفطي. الفحص النسيجي للرغامي يظهر عدم وجود اي مؤشر على تراكم الكاربون في الثلاث مناطق المستخدمه في هذا البحث. بينما في القصبة الهوائية نلاحظ ترسب الكاربون في المناطق الملوثة (منطقة حقل الاحدب النفطي ومنطقة معامل الطابوق) وبكميات مختلقة.


Article
The Effect of Modified Carisolv Gel on Surface Roughness of two Different Types of Ceramic Materials Using Light Polarizing Microscope

Author: Dr. Rasha Hameid Jehad B.D.S., M.Sc. د. رشا حميد
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2009 Volume: 6 Issue: 4 Pages: 307-312
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

This in vitro study evaluated the influence of chemomechanical caries removal solution on the surface topography of metal-ceramic feldspar porcelain (MAJOR ceramic) and All-ceramic feldspar porcelain (Vita Alpha) using light polarizing microscope. Forty specimens of MAJOR ceramic and forty specimens of Vita Alpha ceramic of (12mm diameter & 3mm height) were prepared .All specimens were polished with silicon polishing burs, cleaned, autoglazed and stored in 37°C before exposure to Carisolv. Thirty specimens of each material randomly exposed to Carisolv gel for 5, 10 and 20 minutes respectively, other ten specimens were not, to act as control group. All specimens were subjected to surface roughness test by profilometer and evaluated using light polarizing microscope. ANOVA and student t-test were used to analyze the surface roughness values.Different exposure times of modified Carisolv gel showed highly significant difference compared to control (P<0.001).Surface treatment with modified Carisolv gel for 20 minutes resulted in the highest surface roughness. Metal-ceramic feldspar porcelain (MAJOR ceramic) showed high surface roughness than all ceramic feldspar porcelain (Vita Alpha), however, the difference is statistically not significant (P>0.05).Different exposure times of modified Carisolv gel affect the surface roughness of dental ceramic materials differently. This study showed major influence on surface topography after exposure to modified Carisolv gel for 20 minutes on dental ceramics MAJOR and Vita Alpha.


Article
A scanning electron microscopical study of initial changes in human premolars teeth after orthodontic tooth movement

Author: Dalia Kubad Taher, B.D.S., M.Sc د. داليا طاهر
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2005 Volume: 2 Issue: 2 Pages: 230-234
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

scanning electron microscope was used to study the effect of orthodontic tooth movement by using a force of 50 grams on the pressure side of 2l upper left premolarmt for 5- 75 days while the contralaterl side 14 teeth were used as a control. In order toruiy the morphology and occurrence of root resorptions, the organic tissue was removedrsigg sodium hypochlorite and the denuded root surface were re—examined in thescanning electron microscope. Initially, tissue affected by pressure was mainly located inhe marginal region and subsequently the mid- portion of the roots became generallyuri genced. The resorption process of cementum begins after 5 days as around cavitiesmeasuring about 4u. Further resorption in cementum was characterized by measuringsmall, thin—walled round lacunae which confluence into extensive, shallow resorptionsafter 25 days and more

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