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Article
Concentrations of Major and Minor Elements in Cow’s Milk at Najaf Province

Author: Muneer K. Khudhair
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2016 Volume: 7 Issue: 2 Pages: 9-14
Publisher: University of Kufa جامعة الكوفة

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Abstract

Milk is a very important human nutritive since their consumption has increased in recent years. The aim of this paper was to determine the content of major and minor elements in different milk samples. The current study included measuring the percentages of fat, protein, lactose and solids non fat. Also the present study involved determination some essential minerals in milk, such as measured concentration of calcium, magnesium, phosphorus, sodium, potassium, chloride, and iron. Milk was sampled in March 2016 on ten farms in the Najaf Province directly into plastic bottles (to avoid contamination). The reported data showed that the essential contents of milk of selected farms were have a significant difference and P > 0.05


Article
Determination of bacterial contamination of milk using fluorometry
تحديد التلوث البكتيري للحليب باستخدام الفلورة

Authors: Ayad G.Anwer أياد غازي أنور --- Yasemin Z. Rassam ياسمين زهيرابراهيم رسّام
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2011 Volume: 8 Issue: 3 Pages: 745-750
Publisher: Baghdad University جامعة بغداد

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Abstract

The aim of this paper is to determine the feasibility of using fluorometric methods as an indicator for quality and contamination of milk with E.coli bacteria, and selection the suitable wavelength to be used with laser induced auto fluorescence. Three groups of milk samples were used in this study: Fresh pasteurized milk samples, milk samples containing different concentration of E.coli bacteria which were added artificially, and milk samples that were kept in refrigerator for 3-5 days. Thirteen excitation wavelengths were used to get the emission spectra for all milk samples using spectroflourometer .The results showed that the emission spectra at 275nm excitation wavelength gave a good differentiation between these three groups. The data analysis demonstrated that the proposed method can bring progress in identification of milk quality and contamination with rapidness, high sensitivity and low cost diagnostic tool. Laser light at this wavelength that transmitted by optical fiber can be used for milk samples examination in situe and for more precise results.

الهدف من هذا البحث هو لتحديد امكانية استخدام طرق التفلور كمؤشر لنوعية الحليب وتلوثه ببكتيريا الاشريشيا القولونية وامكانية تحديد الطول الموجي المناسب لاستخدامه في التفلور المحتث بالليزر.أستخدمت في هذه الدراسة ثلاث مجاميع من الحليب ، حليب مبستر وحليب تمت اضافة البكتريا اليه بتراكيز مختلفة ونماذج حليب تم حفضها بالثلاجة لمدة ثلاثة الى خمسة ايام.تم استخدام ثلاثة عشر طول موجي كأطياف تحفيز للحصول على اطياف الانبعاث.اظهرت النتائج بأن اطياف الانبعاث عند الطول الموجي 275 نانومتر ممكن ان تعطي تمييز واضح بين المجاميع الثلاثة . اظهر تحليل البيانات بأن هذه الطريقة يمكن أن تعطي فعالية في التمييز بين عينات الحليب بدقة وسرعة وكلفة قليلة.كما يمكن استخدام الليزر المنقول عن طريق الليف البصري لفحص نماذج الحليب في الموقع بدقة عالية.


Article
Antinociceptive Effect of Silymarin in Experimental Animal

Author: Ahmed S. Sahib احمد صهيب
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2011 Volume: 7 Issue: 2 Pages: 91-94
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Silymarin is a polyphenolic flavonoid derived from milk thistle (Silybum marianum) that has anti-inflammatory, cytoprotective, anticarcinogenic and antioxidant effects. It has been used medicinally to treat liver disorders including acute and chronic viral hepatitis, toxin/drug induced hepatitis, and alcoholic liver disease. Objective: To evaluate the antinociceptive effect of silymarin in experimental animal model of pain.Methods: The efficacy and dose response effect of silymarin (125, 250, and 500mg/kg) were assessed against control using tail flick test in mice as a model of nociceptive pain. In this model, all doses of silymarin were given intraperitoneally 15 min before immersion of tail in hot water 50°C, and Tail Flick Latency was measured before, and after (15, 30, 60 and 120 min) administration of silymarin.Result: Silymarin in 250 and 500mg/kg significantly increase Tail Flick Latency after 15, 30, 60 and 120 min in a dose dependent manner that the maximum effect seen after 120 min compared to baseline value. Conclusion: Silymarin as a herbal drug produce a significant antinociceptive effect in experimental animal model of pain, and beside its better standardization, quality control, and safety profile, in addition to its availability and relative low cost, represent a good alternative choice for management of pain.

Keywords

Silymarin --- milk thistle --- pain


Article
ISOLATION, IDENTIFICATION AND BIOTYPING OF BRUCELLA SPP. FROM MILK PRODUCT AT BASRAH PROVINCE
عزل وتشخيص بكتريا البروسلا من منتجات الألبان في البصرة

Author: Basil A. Abbas Ali B. Talei باسل عبد الزهرة عباس , علي بلبول
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2010 Volume: 9 Issue: 1 Pages: 152-162
Publisher: Basrah University جامعة البصرة

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Abstract

Three hundred milk product samples were collected from different locations of Basrah province. By using enrichment broth technique and Brucella selective medium nine isolates (8 from soft cheese and one from cream) where isolated. No Brucella strain was isolated from ice-cream. The species and biotypes of these isolates were determined and it was found that 4 isolates of Brucella abortus biotype 4 and 5 isolates of B. melitensis biotype 2. Antibiotic sensitivity test showed that all isolates were sensitive to streptomycin, gentamycin, kanamycin, rifampicin, trimethoprim, trimethoprim with sulfamethoxazole and tetracycline, some strains showed resistance to doxycyclin, cephalexin, ampicillin and erythromycin. Brucella isolates also showed growth in different range of temperature and pH.

تم جمع 300 عينة من منتجات الحليب من مناطق مختلفة من البصرة. باستخدام طريقة الاوساط الاغنائية والوسط الانتخابي للبروسيللا تم عزل تسعة عزلات من البروسيلا ثمانية منها من الجبن الطري المحلي وواحد من القيمر المحلي الا انه لم يتم عزل اي عزلة بروسيلا من مرطبات الايس كريم. تم تشخيص هذه العزلات ووجد ان أربعة منها من البروسيلا المجهضة وخمسة منها من البروسيلا المالطية. من خلال دراسة الحساسية الدوائية لها لوحظ ان جميع العزلات كانت حساسة للمضادات streptomycin, gentamycin, kanamycin, rifampicin, trimethoprim, trimethoprim with sulfamethoxazole and tetracycline الا ان اغلب العزلات اظهرت مقاومة للمضادات doxycyclin, cephalexin, ampicillin and erythromycin.. وعند دراسة تاثير الحرارة وال PH لوحظ نمو العزلات في مديات مختلفة من الحرارة وال PH .


Article
THE ROLE OF POWDER MILK AS ACAUSATIVE AGENT OF TYPE ONE HYPERSENSITIVITY AND PREPARATION OF ALLERGY VACCINE.
دور الحليب المجفف كمسبب للنوع الاول من فرط الحساسية وتحضير لقاح الارجية

Author: Othman R.M. رشا منذر عثمان
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2006 Volume: 5 Issue: 1 Pages: 70-76
Publisher: Basrah University جامعة البصرة

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Abstract

The protein extract from powder milk were prepared by extraction, followed bypurification and fractionation using gel filtraction. One peak was obtained from powdermilk with molecular weight of 22KDa.ELISA and skin test were performed on 195 patients tested with powder milk.Therate of positive results to skin test and ELISA was 60.51%.- ‘ ‘ There were significant differences P<0.05 among age groups regarding the numberof patients who had positive skin test and ELISA results ,the mean value of flare diameterand OD-values, in addition, a significant differences P<0.05 occurred among males andfemales examined with powder milk

Keywords

Milk --- ELlSA --- Allergy


Article
ISOLATION AND IDENTIFICATION OF BURKHOLDERIA PSEUDOMALLEI FROM COWS ,GOAT’S MILK AND THEIR SURROUNDING ENVIRONMENT IN BASRAH PROVINCE
عزل وتشخيص جرثومة Burkholderia pseudomaleli من حليب الأبقار والمعز والبيئة المحيطة بها في محافظة البصرة

Author: Adnan .M . AL-Rodhan Hanaa K. Ibrahim عدنان موسى الروضان هناء خليل ابراهيم
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2012 Volume: 11 Issue: 2 Pages: 145-158
Publisher: Basrah University جامعة البصرة

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Abstract

Two hundred samples (cow’s and goat’s milk75 for each,their farm soil 25and 25water samples )from different origin have been cultured on Ashdown selective agar which gave a higher percentage (36.5%)for B. pseudomallei isolation , 64% was observed in farm soil followed by water samples 48% than cow’s milk33.33% and finally the goat’s milk 26.66%. According to L-arabinose biotyping , 39.72% of the B.pseudomallei isolates were positive for arabinose (Arb+) and 60.3% of isolates were negative for arabinose (Arb-).The virulence testing revealed that most bacterial isolates yield haemolysin, protease, lipase and lecithenase in a percentage of 97.3% , 82.2%, 89.04% and 84.9% respectively.The Arb- biotypes were more virulent than Arb+ biotypes with significant differences (P< 0.05).All bacterial isolates were characterized by their ability to form capsule(100%),.This study confirmed the presence of casein proteolytic and necrotizing enzymes in the suspension of B.pseudomallei .Proteins concentration in this suspension was 0.00248 mg/ml .

Keywords

Goat milk --- Arabinose --- Soil


Article
Inhibition effect of Colostrum and breast milk against Shigella flexneri infection
التاثير التثبيطي للبأ والحليب ضد الاصابة بـ Shigella flexneri

Author: Reem Z. Shinashal ريم زهير شنشل
Journal: College Of Basic Education Researches Journal مجلة ابحاث كلية التربية الاساسية ISSN: 19927452 Year: 2013 Volume: 12 Issue: 3 Pages: 753-760
Publisher: Mosul University جامعة الموصل

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Abstract

In this study was undertaken to demonstrate whether colostrum and breast milk exerts any inhibitory effect on bacterial pathogens Shigella flexneri after induction of rabbit orally at concentration 108 cfu/m1, diarrhea appeared in 4-5 days. These cases of diarrhea treated with colostrum and breast milk, we sought the recovery after 12hr. compared with antibiotics (Ampicillin and Cloxacillin) which gives the same result with in 24hr.

تضمنت دراستنا استخدام اللبأ وحليب الصدر كمادة مثبطة للجراثيم المرضية كـ Shigella flexneri وذلك بعد احداث اصابة فموية للارانب بتركيز وحدة تكوين المستعمرة/ مل ومن ثم ظهور علامات الاسهال بـ 5-4 ايام وتم علاج هذه الحالات المرضية بـ اللبأ وحليب الصدر, ظهرت الاستجابة للعلاج بعدـ 12 ساعة من التجريع باللبأ وحليب الصدر, مقارنة بالمضادات الحيوية (Ampicillin and Cloxacillin) التي أعطت نفس النتائج ب24 ساعة.


Article
DIAGNOSTIC STUDY OF EHRLICHIOSIS IN CATTLE OF MOSUL-IRAQ
دراسة تشخيصية للايرليخيوسز في الماشية في مدينة الموصل –العراق

Author: Basima Abdulfatah Al-Badrani باسمة عبد الفتاح البدراني
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2013 Volume: 12 Issue: 1 Pages: 87-97
Publisher: Basrah University جامعة البصرة

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Abstract

Ehrlichiosis (Ehrlichia spp.) was detected in 132(70 Iranian,32 Turkish and 30 local breed)calves (10-14 months old) and 18 dairy cows of local breed (3-5 years old). Animals were brought to Veterinary Teaching Hospital, College of Veterinary Medicine, University of Mosul –Iraq from different farms of Mosul city-Iraq, with clinical signs of fever(40-41C0),anorexia ,emaciation, decrease of milk yield in dairy cows ,paleness of mucous membrane, lymph node enlargement and ticks were detected on different body regions .All blood and buffy coat smears of calves were positive for morula like structures inside the cytoplasm of neutrophils , lymphocytes and monocytes when staining by Giemsa and Wright stains. For all animals , the appearance of Ehrlichia spp. Organisms in leukocytes coincided with erythropenia, leukopenia, and thrombocytopenia and a decrease in hematocrit and hemoglobin concentration. For eighteen dairy cows, Ehrlichia spp. organisms were identified in leukocytes of blood and milk samples. This is apparently the first evidence for Ehrlichia spp. infection in cattle in Iraq. In conclusion, the present study documents that Ehrlichia infection exists in Mosul-Iraq and indicates that the bacterium has been present but unnoticed in the area .Further investigation will be needed in order to characterize different species of Ehrlichia infection in cattle, and explain the role of vector and imported animals in introduction of the disease to our country

Keywords

Ehrlichiosis --- milk. Cows


Article
VARIATIONS IN MILK COMPOSITION OF SOME FARM ANIMALS RESULTED BY SUB-CLINICAL MASTITIS IN AL-DIWANIA PROVINCE.
التغيرات في تركيب الحليب لبعض انواع حيوانات المزرعة نتيجة الإصابة بالتهاب الضرع تحث ألسريري في مدينة الديوانية

Author: Hassan, H.J. هيفاء جمعة حسن
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2013 Volume: 12 Issue: 2 Pages: 17-24
Publisher: Basrah University جامعة البصرة

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Abstract

This study was carried out to evaluate the effect of sub-clinical mastitis and sex of birth on physico chemical composition of milk for some species of farm animals (Cows, Sheep and Goats ) in Al-Diwania province during a period from March to August 2012. A total of (120) samples of raw milk includes (40) samples for cows, (35) samples for sheep and (45) samples for goats were used in this study. The results of this study revealed that there was a significantly difference (P<0.05) in fat and lactose percentages for milk of sheep and goats. Fat percentages were (3.36± 0.24) and (3.33 ±0.16) for milk of infected sheep and goats with sub clinical mastitis respectively as compared to (5.04± 0.14) and(4.20 ± 0.02) for milk of uninfected animals while, the percentages of lactose were (4.27 ±0.02) and (4.30±0.01) for milk of uninfected sheep and goats respectively as compared to (3.91±0.13) and (4.06±0.11) for milk of infected of the same animals. The results also showed a significantly (P<0.05) higher in the solid not fat (SNF) percentage for milk of infected cows. The percentage of SNF was (9.12±0.12) for milk of uninfected cows as compared to (8.78 ±0.08) for milk of infected cows . Protein percentages was a significantly (P<0.05) higher in milk of uninfected cows and sheep , in which was (3.85 ±0.11) and ( 5.75 ±0.11) in both species respectively. The results of this study showed that there was a significantly (P<0.05) higher in PH values of milk for infected of three studied species as compared with milk of uninfected of the same species animals in which was (6.94± 0.06) , (6.71±0.04) and (6.73±0.01) for the three species respectively. The results also demonstrated a significantly (P<0.05) effect of sex of birth on fat and SNF percentages for milk of goats and a significantly (P<0.05) higher of protein percentage in milk for sheep which born male as compared to that born female ( 5.21± 0.35) and (3.89±0.33) respectively.

Keywords

Milk composition --- Sheep --- Goats


Article
ANTIBACTERIAL ACTIVITY OF LAWSONIA INERMIS L .LEAVE EXTRACTS ON STAPHYLOCOCCUS AUREUS ISOLATES
التأثير الضد بكتيري لمستخلصات أوراق نبات الحناء على عزلات المكورات العنقودية الذهبية

Author: Ali A. Al-Edany, Bassam Y. Khudaier, Nawres N. Jaber علي عبود العيداني , بسام ياسين خضير , نورس نوري
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2013 Volume: 12 Issue: 2 Pages: 256-266
Publisher: Basrah University جامعة البصرة

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Abstract

The antibacterial activity of the aqueous, ethanolic and methanolic extracts of Lawsonia inermis (henna) leaves were tested against 46 isolates of Staphylococcus aureus isolated from raw milk, also tested against standard bacteria (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853). The highest antibacterial potency was observed for the methanolic extract with zone of inhibition (14.3043 ± 1.8722 mm), followed by ethanolic (12.9565 ± 2.0106 mm) then aqueous (11.6304 ± 2.2446 mm). The effect of methanolic extract against methicillin resistant S. aureus (MRSA) isolates was the excellent in comparison to other extracts (14.1± 1.88 mm) zone of inhibition followed by ethanolic (12.91 ± 2.372 mm) then aqueous (12 ± 2.41 mm). The isolates were subjected Kirby Bauer method to test their antibiotic susceptibility pattern, substantial antibiotic resistance were shown by 46 (100%) of isolates for ampicillin. Moderate resistance was shown by 31(67.4%) for oxacillin and low resistance was observed by erythromycin. The preliminary phytochemical analysis of the extracts revealed that the presence of high amount of phenolic compounds in methanolic extract (5.4) mg/ml, ethanolic (4.9) mg/ml and aqueoeus (3) mg/ml.MRSA provides a prospecting for new compounds which may be particularly effective against infections that are currently difficult to treat (1).Aims of the conducted study are: 1) an attempt to determine the antibacterial activity of aqueous, ethanolic and methanolic extracts of henna (Lawsonia inermis Linn) leaves against S. aureus isolates, and Gram negative bacteria. 2) antibiotic susceptibility pattern of the isolates. 3) explore the biochemical constituents of extracts.

Keywords

Raw milk --- Bacteria --- methicillin.

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