research centers


Search results: Found 16

Listing 1 - 10 of 16 << page
of 2
>>
Sort by

Article
Assessment of bone marrow angiogenesis using F VIII-related antigen and its relationship to proliferating cell nuclear antigen (PCNA) in multiple myeloma.

Author: Subh S. AL- Mudallal صبح المدلل
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2011 Volume: 53 Issue: 2 Pages: 180-185
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background:Multiple myeloma (MM) is a malignant clonal expansion of plasma cells. Previous studies had demonstrated that both bone marrow angiogenesis as measured by microvessel density (MVD) and PCNA were increased in variety of malignant disorders, including multiple myeloma. Objective: Assessment of angiogenesis in bone marrow of MM patients by using immunohistochemical stain to measure microvessel density (MVD) using factor VIII –related antigen and to evaluate the expression of PCNA antigen in plasma cells of MM bone marrow sections . Also to find the correlation between these two parameters and between them and the percentage of plasma cells infiltration in bone marrow of MM.
Patients, materials andmethods:This retrospective study was conducted from May2007 toJaunary 2008 on 37 bone marrow biopsies diagnosed as multiple myeloma, along with 10 age matched control subjects who had reactive plasmacytosis of less than 10% in their bone marrow. The cases were retrieved from recording archive files of Department of Pathology in the Teaching Laboratory of Medical City Hospital, laboratory of Al- Yarmouk hospital and AL-Yarmouk National Center of Haematology.Three sections were taken from each formalin fixed paraffin embedded bone marrow trephine biopsy of MM and of control cases. One representative section was stained with Hematoxylin and Eosin (H&E), while the other two sections were stained immunohistochemically for factor VIII-related antigen as an endothelial cell marker, and for PCNA as an indicator of the proliferative state of myeloma cells All stained sections were examined by light microscopy and the mean vessel density (MVD) was estimated and was used for statistical analysis. For immunostained PCNA cells, thelabeling index scoring system of Alves et al was adopted for estimating the percentage of positive nuclear staining.
Results:This study revealed that there was a significant increase in bone marrow angiogenesis and in the expression of PCNA in plasma cells of patients with multiple myeloma compared to control cases. Moreover this study showed that PCNA correlated significantly with bone marrow microvessel density and both parameters correlated significantly with the percent of plasma cell in the bone marrow of patient with multiple myeloma.
Conclusion : Both PCNA and angiogenesis as expressed by MVD were increased in multiple myeloma and both of them correlated with the percentage of plasma cell infiltration which reflected the activity of the disease .Moreover the proliferative activity of plasma cell as represented by PCNA expression was closely related to angiogenic activity , thus it can be proposed that both markers reflect the disease activity and they may provide additional information when included in the initial evaluation of myeloma bone marrow biopsies .


Article
Plasmacytoma and multiple myeloma in Basrah province

Author: Salim L. AL-Saad, Jasim.M. AL-Diab,Osama G. AL-Asady
Journal: The Medical Journal of Basrah University المجلة الطبية لجامعة البصرة ISSN: 02530759 Year: 2013 Volume: 31 Issue: 2 Pages: 75-78
Publisher: Basrah University جامعة البصرة

Loading...
Loading...
Abstract

Objective: to study trends of multiple myeloma and plasmacytoma in Basrah as a model of Asian countries and compare the results with those of western records.Methods: retrospective review of 102 cases of multiple myeloma and plasmacytoma of both sexes registered in Basrah through the years 1996-2010 regarding their demographic features the collected dated tabulated and analyzed.Results: a total of 102 patient, 62 males and 40 females with a median age for males 56 years and for females 55 years multiple myeloma represented 92.2% of total cases while plasmacytoma represented 7.8%.Conclusion: -Incidence rate of multiple myeloma and plsmacytoma in eastern countries seems comparable to incidence rate in eastern countries but the incidence is low when compared to western statistics.-Multiple myeloma and plasmacytoma showed no significant changes through the last 15 years.


Article
Survival advantage of adding thalidomide as compared with chemotherapy only in the treatment of symptomatic myeloma in Kurdistan/Iraq

Author: Ahmed K. Yassin
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2014 Volume: 3 Issue: 2 Pages: 75-84
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Multiple myeloma is an incurable plasma cells neoplasm. Chemotherapy by thealkylating agent Melphalan was introduced in 1958 and with the addition of prednisolone in1964 the combination (MP) improved the survival of these patients and remained the standardtreatment for over twenty years. In 1998 the antiangiogenic reagent thalidomide was tried andclaimed to further improve survival of these patients including those with advanced disease.Objectives: This study was conducted to determine the difference in survival of multiplemyeloma patients in Nanakaly Hospital in Erbil city before and after the use of thalidomide.Patients and Methods: This retrospective study was carried out at Nanakaly Hospital over aperiod of six years from January 2007 to March 2013. The records 123 patients with symptomaticmultiple myeloma who were treated in this period were reviewed, including their clinical andlaboratory profiles, as well as the survival of each group. All patients were selected according tothe WHO diagnostic criteria.Results: The patients had a median age of 59 years and the male: female ratio of 1.2:1,Chemotherapy based treatment was used in 38 patients as compared with 85 patients who usedthalidomide based therapy.Median survival was 34 months for thalidomide compared to 19 months for chemotherapy-onlytreated group (P= 0.001)Conclusions: The introduction of Thalidomide as a therapeutic option in Iraqi patients improvedsurvival of symptomatic myeloma significantly compared to chemotherapy alone.

Keywords

Multiple myeloma --- thalidomide --- Erbil --- Iraq.


Article
The value of fine needle aspiration cytology in the diagnosis of oral and jaw lesions in patients with plasma cell dyscrasias

Author: Bashar H. Abdullah بشار عبد الله
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2005 Volume: 17 Issue: 2 Pages: 41-44
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Fine needle aspiration cytology (FNAC) has been found to be very useful for the diagnosis of lesions as multiple myeloma (MM) and plasmacytomas in different parts of the body. The usefulness of such procedure has not yet been verified in the oral and maxillofacial region. This study was conducted to verify the value of FNAC in the diagnosis of oral and maxillofacial lesions in patients with plasma cell dyscrasias.Patients and methods: After clinical and radiological examination, FNAC was done by the use of 10 cc syringes with 22-23 gauge needles on 11 patients with lesions affecting the maxillofacial region. The smears stained with Leishman’s stain, to be examined microscopically.Results: Twenty eight patients were examined throughout 1.5 years period, 11 had soft tissue/or bone lesions affecting the maxillofacial region. 9 patients with MM and 2 had plasmacytoma. Of 9 patients with MM, the mandible was involved in 5 patients, 3 lesions affected the maxilla, and while in one patient the maxilla and mandible were both affected. Soft tissue lesions were seen in 6 patients. The results of FNAC showed that all lesions were due to involvement with myeloma cell infiltrates, with one exception of 2 osteolytic lesions which were due to odontogenic infection.Conclusion: FNAC is a very useful and safe procedure to diagnose different types of lesions affecting the oral cavity in patients with plasma cell dyscriasis


Article
The role of TNF-α in the pathogenesis of multiple myeloma “a study in Iraqi patients”
دورعامل نخر الورم الفا (TNF-α) في إمراضية الورم النخاعي المتعدد " دراسة لمرضى عراقيين "

Authors: Mohammed A. Essa محمد أحمدعيسى --- Sabah N. Mohammed صباح نعمة محمد --- Talib A. Hussein طالب عبد الله حسين --- Azhar J. Mohsin أزهار جاسم محسن
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2014 Volume: 11 Issue: 2 عدد خاص بالمؤتمر النسوي الثاني Pages: 853-860
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

During recent years, there has been an increasing interest in the investigation of the cytokines roles in pathogenesis of cancer, thus the study aimed at evaluating the level of tumor necrosis factor-alpha(TNF-α) in sera of Iraqi multiple myeloma (MM) patients. Beta 2-microglobulion (β2-m) was assessed to determine if there was any association between this cytokine and the level of β2- m, as the latter is related to the stage of the disease. In addition, the age and gender were also taken into consideration. Furthermore, we investigated the relationship between IgG and TNF-α in sera of patients. 49 Iraqi patients (27 males and 22 females).The patients were also divided into two groups: the first group included (17) patients who were recently diagnosed and not received any treatment at the time of collecting samples while the second group included (32) patients who received treatment. A further group was also investigated which included (12) apparently healthy individuals (9 males and 3 females), who were regarded as a control group. Serum TNF-α and β2- m were determined using enzyme-linked immunosorbent assay (ELISA).while the concentration of IgG was measured by radial immune diffusion plates The study reached to the following results: TNF-α levels were not significantly elevated in the patients with MM compared to control group (5.98 ± 8.47 SD vs. 4.85 ± 12.1 SD) and no significant differences (P > 0.05) were observed in the mean (6.02 ±8.1SD vs 5.9 ±9.4SD) concentration of TNF-α in patients with MM who received treatment, when compared with those who did not take the treatment .In addition, there are positive significant correlations between TNF-α and β 2 Microglobulin (r = 0.316, P = 0 .027), and no relationship between IgG and TNF-α (r = - 0.032, P = 0.829).Furthermore, the study observed that, there was no correlation between TNF-α on the one hand and factors of age and gender on the other hand.

تزايد الأهتمام خلال السَنَوات الأخيرة، في التحرّي عن أدوارِ الحركيات الخلوية في تولّد مرض السرطان ومنشأه ، وهكذا إستهدفتْ الدراسة الحالية تَقييم مستوى عاملِ نخرِالورمِ الفا (TNF-α) في مصول مجموعة من مرضى الورم النخاعي المتعدد في المرضى العراقيين. كما تمّ تقييم مستوى β2-m والتحري عن العلاقة بين عامل نخر الورم الفا TNF- α و β2-m لإرتباط الأخير بمرحلة المرض.إضافة الى ذلك اُخذت الدراسة بنظر الاعتبار عاملي العمر والجنس.فضلا عن ذلك تمّ التحري عن العلاقة بين تركيز عامل نخر الورم الفا TNF-αمن جانب والغلوبيولين المناعي من نوع (IgG) من جانب آخر.شملت الدراسة 49 مريض (27 ذكر و22 انثى )، هؤلاء المرضى قسموا الى مجموعتين تضمنت المجموعة الاولى (17 مريض ) من المرضى المشخصين حديثا والذين لم يتلقوا اي علاج في وقت جمع العينات ، في حين تضمنت المجموعة الثانية (32 مريضاً) كانوا خاضعين للعلاج. ايضاً تضمنت الدراسة مجموعة اخرى وهي المجموعة الضابطة والتي تشمل 12 شخص من الاصحاء(9 من الذكور و3 من الاناث).تمَّ قياس مستوى عامل نخر الورم الفا TNF- α و β2-mبأستخدام تقنية المُمْتَزِّ المناعي المُرْتَبِطِ بالانزيم ELISAفي حين استخدمت طريقة اطباق الانتشار المناعي الشعاعي لقياس تركيز الغلوبيولين المناعي نوع (IgG).توصلت الدراسةالى النتائج التالية :لايوجد اختلاف في مستوى تركيز عامل نخر الورم الفاTNF- α بين مجموعة المرضى والمجموعة الضابطة ) 5.98± 8.47 مقابل 4.85 ±12.1 بيكو غرام مليلتر) ،كما لايوجد اختلاف ذو دلالة معنوية بين مجموعة المرضى اللذين تلقوا علاج مقابل مجموعة المرضى المشخصين حديثاً (6.02 ± 8.1 مقابل 4.85 ±12.1 ).أضافة الى ذلك لوحظ وجود علاقة معنوية موجبة بين عامل نخر الورم الفاTNF- α وβ2-m حيث كان معامل الارتباط (r =0.316 ، P =0.027) كمـا توصلت الدراسة الى عدم وجود عـلاقة بين عامل نخر الورم الفاTNF- α و الغلوبيولين المناعي من نوع (IgG) حيث كان معامل الارتباط (r = - 0.032،P = 0.829 ) ،أخيراً أثبتت الدراسة عدم وجود علاقة معنوية بين انتاج TNF- α من جهة وعاملي العمر والجنس من جهة اخرى كما لايوجد تأثير للجنس على انتاج هذا النوع من الحركيات الخلوية.


Article
Survival of patients with multiple myeloma diagnosed at the national center of hematology in Baghdad
بقاء المرضى الذين يعانون من ورم نقي العظم المتعدد التي تم تشخيصها في المركز الوطني لأمراض الدم في بغداد

Author: Alaa Fadhil Alwan علاء فاضل علوان
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2014 Volume: 7 Issue: 2 Pages: 133-139
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Multiple myeloma (MM) is a monoclonal malignant proliferation of plasma cells derived from a single clone. Due to the variety of organ dysfunction caused by this malignant disease with no curative therapy, hence survival becomes a challenge for this group of patients. The aim of this study was to analyze the epidemiology and survival of patients with MM attended the out-patient clinic of the national center of hematology in Baghdad/Iraq. A retrospective study conducted at the national center of hematology, from September 2009 to November 2013 in which the medical records of 46 patients with MM have been reviewed. Survival analysis was estimated by the Kaplan-Meier and multivariate Cox regression analysis. The mean age was 63.4 years, and 54.2% of patients studied were female. The most common clinical manifestations were anemia (93.4%), bone pain (86.9%), and renal impairment (39.1%). In survival analysis, the only variable that achieved statistical significance was renal impairment (p = 0.025). For mortality, renal impairment (p = 0.017) and coagulation abnormalities (p = 0.012) were significant in the Cox regression. In conclusion the epidemiological profile showed a slight predominance in females. Anemia and bone pain were the most frequent complaints. Renal impairment and coagulation abnormalities were associated with mortality for patients with multiple myeloma.

ورم نقي العظم المتعدد هو تكاثر للخلايا الخبيثة وحيدة النسيلة من خلايا البلازما المستمدة من استنساخ واحد. يسبب هذا المرض خلل في كثير من وظائف الاعضاء الحيوية للجسم ويرجع ذلك إلى مجموعة متنوعة من ضعف جهاز المناعة الناجمة عن هذا المرض الخبيث ويصبح بالتالي بقاء المريض تحديا لهذه الفئة من المرضى. وكان الهدف من هذه الدراسة هو تحليل وبائيات وبقاء المرضى الذين يعانون من ورم نقي العظم المتعدد الذين يراجعون العيادة الخارجية للمركز الوطني لأمراض الدم في بغداد / العراق. دراسة استعادية أجريت في المركز الوطني لأمراض الدم، في الفترة من سبتمبر 2009 إلى نوفمبر 2013 حيث تم استعراض السجلات الطبيةل 46 مريضا 46 مصاب بورم نقي العظم المتعدد وتم تحليل البقاء من قبل كابلان ماير والتحليل متعدد المتغيرات كوكس والانحدار. كان متوسط العمر 63.4 عاما، وكانت 54.2٪ من المرضى الخاضعين للدراسة من الإناث. كانت المظاهر السريرية الأكثر شيوعا فقر الدم (93.4٪)، وآلام العظام (86.9٪)، والقصور الكلوي (39.1٪). في تحليل البقاء على قيد الحياة، كان المتغير الوحيد التي حققت دلالة إحصائية القصور الكلوي (p= 0.025). لفيات، كان القصور الكلوي p)= 0.017) وشذوذ تخثر (p= 0.012) كبير في الانحدار كوكس. في الختام أظهر الوبائية غلبة طفيفة في الإناث. كان فقر الدم وآلام العظام الشكاوى الأكثر شيوعا. ارتبطت ضعف الكلى وشذوذ تخثر مع فيات لمرضى


Article
RELATIVE PLASMA VISCOSITY: A SIMPLE TEST FOR THE BED SIDE DIAGNOSIS OF MULTIPLE MYELOMA
استعمال ممصة كريات الدم الحمراء لتشخيص الورم النقيي المتعدد بجانب السرير

Author: Falih S. Sarhan فالح سالم سرحان
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2006 Volume: 5 Issue: 1 Pages: 40-43
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background: The measurement of plasma viscosity (PV) has long been recognized to be important for the diagnosis of paraproteinemia and it is widely used recently for the assessment of peripheral vascular disorders, diabetes mellitus and malignant diseases. The WHO recommended method for measuring PV is the electronic Harkness viscometer, but it is expensive and not widely available.Objective: A simplified, cheaper and accurate technique using the red cell pipette is worth trying as a rapid bedside test.Methods: The relative plasma viscosity (RPV) was measured in 30 patients with multiple myeloma and 150 healthy adults. The method applied was that of Wright and Jenkins in which a comparison of the vertical flow of plasma to distilled water using the red cell pipette is used to measure the relative viscosity of plasma. The erythrocyte sedimentation rate for both the patients and control was performed for comparison. Results: The mean RPV in multiple myeloma patients was highly raised compared to normal and that increment is highly significant statistically (p<0.001). These results are considered highly supportive of the diagnosis of myeloma. In the other hand, although the results of ESR were statistically significant, it cannot be differentiated from those due to other disorders. Conclusion: The measurement of RPV has proved to be simple and reliable and may be used at the bed side to detect the activity and to assess the diagnosis of multiple myeloma.Key words: Plasma viscosity, multiple myeloma.

خلفية الدراسة: يعتبر قياس لزوجة البلازما من التحاليل المهمة في تشخيص الامراض المتزامنة بنظائر البروتين. كما ان استعمالها قد ازداد انتشارا في تشخيص و تقييم اضطرابات الاوعية الدموية المحيطية، مرض السكري و الامراض السرطانية المختلفة. لقد اوصت منظمة الصحة العالمية باستعمال جهاز هاركنس الالكتروني لقياس اللزوجة البلازمية، غير ان هذا الجهاز مكلف و غير متوفر على نطاق واسع.هدف الدراسة: لذلك فان استعمال جهاز مبسط، رخيص و دقيق في نفس الوقت كممصة كريات الدم الحمراء، امر يستحق التجربة من اجل التشخيص السريع (على جانب السرير) للورم النقيي المتعدد.طريقة العمل: تم قياس اللزوجة النسبية للبلازما باستعمال ممصة كريات الدم الحمراء في 30 مريضا بالورم النقيي المتعدد و 150 شخصا سليما بالغا نصفهم نساء و نصفهم رجال. كنوع من المقارنة قمت بقياس معدل ترسب كرات الدم الحمراء لاثبات ان قياس قياس اللزوجة النسبية للبلازما يعطي فوائد كثيرة كما و يعتبر تشخيصي لوجود نظير البروتين.النتائج: اثبتت النتائج ان اللزوجة النسبية للبلازما و معدل ترسب كرات الدم الحمراء في مرضى الورم النقيي المتعدد كانت مرتفعة بشكل كبير عن نتائج الاشخاص الطبيعيين مع فرق بالغ الجدوى احصائيا. ان نتائج اللزوجة النسبية للبلازما في الورم النقيي المتعدد يمكن اعتبارها نتائج مساندة و تشخيصية على عكس نتائج معدل ترسب كرات الدم الحمراء التي قد توجد في الكثير من الامراض الاخرى.الاستنتاج: لقد تم اثبات ان قياس اللزوجة النسبية للبلازما باستعمال ممصة كريات الدم الحمراء تعتبر طريقة بسيطة و معتمدة و يمكن استعمالها في جانب السرير لتشخيص و فحص نشاط الورم النقيي المتعدد. مفتاح الكلمات: لزوجة البلازما، الورم النقيي المتعدد


Article
A Study of Serum Protein Electrophoresis in Patients with Multiple Myeloma

Author: Hind Shakir Ahmed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 4 Pages: 494-498
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT: BACKGROUND: Multiple myeloma (MM) is caused by the malignant proliferation of plasma cells through the bone marrow. The clinical features are due to disordered immunoglobulin synthesis and/or secretion from the cell. The amount of protein in the vascular compartment depends on the balance between the rate of synthesis and catabolism or loss. Electrophoresis is a technique that separates compounds such as proteins according to their different electrical charge. In this way, five main groups of proteins namely albumin and the α1, α2, β, γ-globulins, may be distinguished after protein staining and may be visually compared with those in a normal control serum. OBJECTIVE: To evaluate total serum protein, serum albumin, serum globulin, and serum protein electrophoresis in Iraqi MM patients. PATIENTS AND METHODS: This study was conducted during the period from August 2012 until the end of November 2012. The patients were admitted to Medical City Hospital and all the measurements were performed in the Medical Teaching Laboratories in Baghdad. Total serum protein was measured by colorimetric Biuret method, while serum albumin was measured by bromocresol green method, serum globulin, and serum protein electrophoresis were measured in 25 patients with MM; their age range was (50-70) years and compared with 25 healthy controls.RESULTS: Total serum protein and serum globulin were significantly increased (86.76±17.05 g/l and 55.36±20.54 g/l respectively) in patients with MM, as compared with their controls, (P=0.0001). While a significant decreased was found in serum albumin (29.6±8.77 g/l) and albumin to globulin ratio (0.64±0.36 g/l) for patients with MM as compared with their controls, (P=0.0001). CONCLUSION:Abnormal concentrations of total serum protein, serum albumin, serum globulin, and M-band are associated with MM


Article
The effect of Punica granatum nanoparticles in phagocytic cells in patients with Multiple Myeloma
تأثيرالحبيبات النانوية لمستخلص قشور الرمان على الخلايا البلعمية لمرضى المايلومة المتعددة

Authors: Majid Sakhi Jabir --- Zainab Jihad Taqi --- Imman Ismael Jabbar --- Mohammed Shammel Ali --- et al.
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2015 Volume: 33 Issue: 9 Part (B) Scientific Pages: 1702-1711
Publisher: University of Technology الجامعة التكنولوجية

Loading...
Loading...
Abstract

Multiple myeloma is a cancer that forms in a type of white blood cell called a plasma cell. Plasma cells help you fight infections by making antibodies that recognize and attack germs. This study aimed to investigate effect of aqueous extract and silver nanoparticles from Punica granatum peel on the ability of phagocytic cells in patients with Multiple myeloma. Chemical components of Punica granatum were investigated. It was found that they contained most of chemical compounds such as Glycosides, Flavonoids, Alkaloids, Saponins, Tanins, Resins, Terpenes, and Steroids. Our results showed the presence of a significant decrease (p˂0.001) in ability of phagocytic cells in patients, when the results were compared to the control group which included healthy individuals. While, the results showed increase in the percentage of phagocytic cells after added Punica granatum peel silver nanoparticles by increases activity of NADPH enzyme. This increase indicates that the Punica granatum peel silver nanoparticles contain chemical components which act as immune modulators by increase of the percentage of phagocytic cells to engulf bacteria.

المايلومة المتعدده هو مرض سرطاني يصيب الخلايا البلازمية مما يؤدي الى تكاثر هذه الخلايا بشكل غير طبيعي وتكدسها في نخاع العظم ويشكل نسبة 10% من الاصابات بسرطان الدم. هيأت النباتات الطبية بمختلف انواعها قاعدة اساسية ومهمة لأنظمة الطب التقليدية اذ تتم الاستفادة منها في توفير بعض انواع العقاقير التي تتميز بخلوها من الجوانب السلبية المؤثرة على اعضاء جسم الانسان على العكس من العقاقير الكيميائية التي لاتخلو من بعض تلك الجوانب.لذلك هدفت الدراسة الحالية الى أختبار تأثير الجزيئات النانووية لمستخلص قشور الرمان المائي في فعالية الخلايا البلعمية لمرضى المايلومة المتعددة Multiple myeloma , اذ تم تحضير المستخلص المائي لقشور الرمان وكذلك تم تحضير الجزيئات النانووية بطريقة نترات الفضة وتم التحري عن المحتوى الكيميائي للمستخلص المائي لقشور الرمان فوجد احتواءه على مركبات كيميائية عديدة مثل الكلايكوسيدات والفلافونات والقلويدات والصابونيات والتانينات والتربينات والراتنجات والسترويدات. تم أختبار فعالية الخلايا البلعمية على الالتهام لمرضى المايلومة المتعددة ومجموعة السيطرة قبل وبعد اضافة المستخلص المائي والجزيئات النانووية لقشور الرمان. أظهرت نتائج الدراسة الحالية قبل اضافة المستخلص وجود انخفاض معنوي كبير P˂0.001 في فعالية الخلايا البلعمية على الالتهام لمجاميع المرضى عند مقارنة النتائج بمجموعة السيطرة. أما بعد أضافة المستخلص المائي والجزيئات النانووية لقشور الرمان فقد أظهرت النتائج أرتفاعا ملحوظا في فعالية الخلايا البلعمية عن طريقة زيادة فعالية انزيم NADPH.هذه الزيادة قد تكون دليل على فعالية الجزيئات النانووية لقشور الرمان وعملها كمحورات مناعية من خلال زيادة النسبة المؤوية لقابلية الخلايا البلعمية على الالتهام.


Article
Serm Immuno fixation Electrophoresis as a diagnostic method for monoclonal gammopathies in patients with multiple myeloma

Authors: Araz Muhammad Yousif --- Nashwan Sleman Ablhad --- Parween Abdulsamad Ismail
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2018 Volume: 29 Issue: 4 Pages: 43-49
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

B cells clonal expansion (producing abnormal amounts of immunoglobulins) reflect conditions causes a group of disorders called monoclonal gammopathies. They may be appearing as a range of diseases that consist of multiple myelomas (MM). The aim of this research is to use quantify and identify monoclonal gammopathy by serum immunofixation electrophoresis (SIFE) beside the serum protein electrophoresis (SPEP) assay as a tumor marker in the diagnosis of suspected multiple myeloma cases. Serum samples were collected from 94 patients with MM, and 30 persons as control, SPEP and SIFE were used for both groups. M band determined and evaluated of M protein by the Hellabio instruments. The results of this study showed significant elevation (p<0.001) in the group of patients with MM compared to control as follows: 49 (52.13%) for IgG kappa, 23 (24.46%) for IgG lambda, 10 (10.63%) for IgA kappa, 4 (4.25%) for IgA lambda, 6 (6.38%) for IgM kappa, and 2 (2.12%) for IgG monoclonal gammopathy. SPEP ought to be proposed as the original test for the establishing of doubted cases of multiple myeloma. IFE is the gold mark now and ought to be performed to certify the existence of an M-protein and to diagnostic its light chain and heavy chain isotype, which also leads to increase the sensitivity of diagnosis in suspected multiple myeloma cases by using SIFE beside the SPEP assay and utilized for detection and quantification of monoclonal gammopathy.

توسع الخلايا البائية (إنتاج كميات غير طبيعية من الجلوبيولينات المناعية) تعكس الظروف التي تؤدي إلى مجموعة من الاضطرابات التي تسمى اعتلال gammopathies أحادي النسيلة. قد تظهر على أنها مجموعة من الأمراض التي تتكون من الأورام النقوية المتعددة (MM). الهدف من هذا البحث هو استخدام القياس الكمي وتحديد اعتلال غامض وحيدة النسيلة(monoclonal gammopathy) عن طريق الترحيل الكهربائي لمصل المصل (SIFE) بجانب فحص الترحيل الكهربائي البروتين في الدم (SPEP) كمؤشر للورم في تشخيص حالات المايلوما المتعددة المشتبه فيها. تم جمع عينات من المصل من 94 مريضا مع MM ، 30 شخصا كمجموعة السيطرة ، وتم استخدام SPEP و SIFE لكلا المجموعتين. حددت حزمةM ا وتقييم البروتين M بواسطة جهازHellabio. أظهرت نتائج هذه الدراسة ارتفاع معنوي (p<0.001) في مجموعة المرضى الذين لديهم MM مقارنة بمجموعة السيطرة كما يلي: 49 (52.13٪) لـ IgG kappa ، 23 (24.46٪) لـ IgG lambda ، 10 (10.63٪). ) لـ IgA kappa ، 4 (4.25٪) لـ IgA lambda ، 6 (6.38٪) لـ IgM kappa ، و 2 (2.12٪) لـ IgG monoclonal gammopathy. ينبغي اقتراح SPEP كاختبار أساسي لإنشاء حالات مشكوك فيها من المايلوما المتعددة. IFE هي العلامة الذهبية الآن ويجب أن يتم تنفيذها للتأكد من وجود بروتين M ولتشخيص سلسلته الخفيفة وسلسلة isotype الثقيلة ، مما يؤدي أيضًا إلى زيادة حساسية التشخيص في حالات المايلوما المتعددة المشتبه فيها باستخدام SIFE بجانب اختبار SPEP واستخدمت للكشف عن وتقدير الكمي لاعتلال الأعصاب وحيدة النسيلة.

Listing 1 - 10 of 16 << page
of 2
>>
Sort by
Narrow your search

Resource type

article (16)


Language

English (14)

Arabic and English (2)


Year
From To Submit

2018 (1)

2017 (1)

2015 (1)

2014 (5)

2013 (3)

More...