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Article
Histological Study on the Effect of Methotrexate on the Oral Tissues of Aadult Male Rabbits

Authors: Alhan D Al-Moula --- Mareb H Ahmed --- Makarem H Ahmed
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2013 Volume: 13 Issue: 3 Pages: 432-441
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The objective of this study is to evaluate the effects of oral methotrexate on the oral tissues of rabbits. Materials and Methods: In the present study nine adults' male rabbits, aged 3 months were used, and divided into three main groups. Group 1: served as a control and received normal saline only. Group II: given a single dose of 0.5mg/kg per week methotrexate orally by cavage needle for 8 weeks. Group III: given 0.5mg/kg of methotrexate orally twice/weekly for 8 weeks. The animals were scarified and the floor of the mouth, check and tongue, were excised and processed for histological study. Results: The epithelium of oral mucosa in the treated groups is thinner than that of the control group with vacuolation in the cytoplasm, fewer and shorter slender papillae ,congestion of the blood vessels in the lamina propria , fatty infiltration and distortion of attachments of mucosa to underlying muscle .The minor salivary glands show loss of normal architecture ,inflammatory cellular infiltration, disarrangements of acini, and vacuolation in the acinar cells .The dorsal surface of the tongue showed an epithelial atrophy, with loss of the lingual papillae . The lamina propria showed short papillae, inflammatory cellular infiltration and congestion of blood vessels, as well as considerable distortion of arrangements in the musculature of the tongue. Conclusion: weekly administrations of methotrexate produce dramatize histological changes in the oral tissues.

Keywords

Methotrexate --- oral mucosa --- rabbits.


Article
Localized hyperplastic lesions of the oral mucosa: A clinico - pathological study

Author: Sabah M SAKA
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2001 Volume: 1 Issue: 2 Pages: 113-128
Publisher: Mosul University جامعة الموصل

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Abstract

A retrospective study of (96) cases of mucosal hyperplastic lesions of the oral cavity was carried out. The distribution of the lesions in relation to the age, sex, size and the site of origin was performed. The result shows that these lesions affect similar age groups except denture induced hyperplasia, which affect older age group Females were affected more than males especially for peripheral giant cell granuloma and denture induced hyperplasia, Fibro - epithelial polyp and fibrous epulis show smaller size than other types of the lesions. Majority of pyogenic granuloma and peripheral giant cell granuloma arised in the posterior part of the jaws, by contrast to other type of the lesions which show no difference in their site of origin.


Article
Histopathological evaluation of human amniotic membrane effect on early stage healing of induced defects in rabbit's oral mucosa

Authors: Dr. Rasha F. Albannaa. * د. رشا البنا --- Dr. Fahad M. Al-Dabbagh. ** د. فهد --- Dr. Manar M. Alnema. *** د. منار النعيمي
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2013 Volume: 10 Issue: 2 Pages: 252-257
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

This study aims to investigate the role of using dried Human Amniotic Membrane(dHAM) on rabbit's oral mucosal wound healing in its early stages. Six male rabbitswere used in this study. In each rabbit, two elliptical incisions were done on both sidesof tongue dorsum; one of them was left uncovered and the other was covered bydHAM. The animals were divided into two groups with three rabbits each accordingto the sacrifice periods which were three days and one week. After sacrifice,histopathological examination was performed to assess re-epithelialization andinflammation. Within the first three days, the re-epithelialization was much morefaster in dHAM group than that in control one, and the difference between the controland study group was statistically highly significant ( p=0.005 <0.01). Moreover, therate of inflammatory process show significant difference between the two groups withadvantage to HAM group (p =0.046 <0.05). However, after one week, this differencewas statistically non-significant ( p =0.317 > 0.05). Human Amniotic Membranecould be considered as an option to enhance oral wound healing especially in its earlystages.


Article
ASSOCIATION OF COMPLEMENTFACTOR H GENE (CFH) SINGLE NUCLEOTIDE POLYMORPHISM WITH RECURRENT APHTHUS STOMATITIS
أرتباط تعدد الأشكال النوكليوتيدي لمورثة CFH مع ألتهاب الفم القلاعي المتكرر

Authors: ABDULRAZZAQ MOHAMMED عبدالرزاق محمد عبدالرحمن --- AHMED MOHAMMED SALIH احمد محمدصالح --- SOLEEN SARDAR ZUHDIN سولين سردار زهدي --- HALIZ SADDEQ HASAN هلز صديق حسن
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2018 Volume: 12 Issue: 1 Pages: 84-95
Publisher: University of Dohuk جامعة دهوك

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Abstract

ABSTRACTBackground: Recurrent aphthous stomatitis (RAS) is a common chronic inflammationaffecting oral mucosa that lead to mucosal ulceration. The causes are unclear, butdysregulation of the immune response has been proposed to be implicated in the developmentof the disease. In this study, we hypothesized that RAS is provoked by the dysregulation ofthe complement system, through the impairment of the function of complement regulatoryproteins, the present study has aimed to investigate the impact of CFH gene SNPs thatencodes the production of complement factor H in RAS development.Subject and Methods: Blood samples from 46 patientswith recurrent aphthus stomatitiswere collected including 35 males and 11 females and 46 age and sex matched apparentlyhealthy (with healthy oral cavity) volunteersincluding 23 males and 23 females were involvedas a control group. Gnomic DNA was extractedfrom each blood sampleusing theisopropanol/ethanol method. Specific primers were used to amplify the CFH gene fragmentthat harbors the rs1061170 site encoding theTyr402 aminoacid. The PCR products weredigested with NlaIII restriction enzyme.Results: A significant difference was found between the age groups among the RAS patientsin regard to the severity of and recurrence of the RAS episodes, it was found to be highersignificantly among the age group (20-30 years) compared to other age groups among RASpatients. Out of the 46 RAS patients, the CFH single nucleotide polymorphism (SNP),Tyr402His polymorphism variant was detected in 18 (39.1%); 11 (23.9%) females and 7(15.2%) males, represented as 8 (17.4%) Tyr/His heterozygous variants and 10 (21.7%) wereHis/His homozygous variant. Among the 46 healthy control group, the CFH single nucleotidepolymorphism (SNP), (Tyr402His polymorphism variant) was detected in 6 (13%) all ofthem were Tyr402His variant, 2 (4.3%) males and 4 (8.7%) females. There was a significantdifference in the CFH (Tyr/His, His/His) variants rates between the RAS group and thehealthy control group (p <0.05), but there was no significant difference of CFH (Tyr/His,His/His) variants rates between the males and females in the RAS group.Conclusions: We suggest that Tyr402Hispolymorphism can be considered as a risk factor forthe RAS development, and His204His variant is more associated with the disease, however,more studies are recommended to be conducted on a larger sample size to confirm theseevidences.

الخلفية والأهداف: ألتهاب الفم القلاعي المتكرر هو التهاب مزمن شائع يؤثر على الغشاء المخاطي للفم الذي يؤدي إلى تقرح الغشاء المخاطي. الأسباب غير واضحة، ولكن من المعتقد أن الاستجابة المناعية قد تكون مسؤولة عن تطور المرض. في هذه الدراسة، افترضنا أن المرض ينتج عن استفزاز من قبل ضعف تنظيم فاعليةالنظام المناعي المكمل، من خلال ضعف وظيفة البروتين التنظيمي التكميلي. في هذه الدراسة كان الهدف هو التحقق من تأثير الأشكال الجينية المتباينة للجين الذي يشفر إنتاج عامل المناعة التكميلي Hعلى نشؤ المرض.طرق البحث: تضمنت الدراسة مجموعة مكونة من 46 مريضا مصابا بالتهاب الفم القلاعي المتكرر، بينهم 35 ذكور و 11 إناث و 46 متطوعا أصحاء كمجموعة مقارنة (لا يعنون من أية مشاكل صحية في الفم) من بينهم 23 ذكور و 23 من الإناث. جمعت عينات الدم من كل منهم، وتم استخراج الحمض النووي الجيني باستخدام طريقة الأيزوبروبانول / الإيثانول. واستخدمت طريقة تفاعل البلمرةالتسلسلي لتضخيم جزء الجينيشفر إنتاج عامل المناعة التكميلي Hوالذي يؤوي موقع rs1061170 ويشفر موقع الحامض الأميني 2Tyr40. تم تقطيع نواتج تضخيم الحامض الأميني بأستخدام انزيم NlaIII ألهاضم للحامض النووي .النتائج: تم العثور على فرق معنوي بين الفئات العمرية لدى مرضى التهاب الفم القلاعي فيما يتعلق بشدة وتكرار نوبات المرض، فقد وجد أن المرض يتكرر بصورة أعلى بكثير بين الفئة العمرية (20-30 سنة) مقارنة مع الفئات العمرية الأخرى بين المرضى. من أصل 46 مرضى ، تم الكشف عن تعدد الأشكال النيوكليوتيدية المتباينةTyr402 His في 18 (39.1٪), 11 (23.9٪) من الإناث و 7 (15.2٪) من الذكور، و 8 (17.4٪) Tyr402His متغايرةالزايكوت، و 10 (21.7٪) كان His 402His متماثل الزايكوت. ومن بين 46 مجموعة السيطرة الصحية، تم الكشف عن تعدد الأشكال النكليوتيدات (Tyr402His في 6 (13٪) وكان جميعهم يحملون Tyr204His المتباينة الزايكوت، 2 (4.3٪) من الذكور و 4 (8.7٪) من الإناث. كان هناك فرق معنوي في معدلات المتغيرات (Tyr402His ، His 402His) بين مجموعة المرضى ومجموعة المقارنة (p<0.05). ولكن لم يكن هناك فرق معنوي في معدلات المتغيرات (Tyr402His ، His 402His) بين الذكور والإناث في مجموعة المرضى.الاستنتاجات : يمكن اعتبارطفرة Tyr204His في مورثة CFH عامل خطورة لظهور مرضى التهاب الفم القلاعي ، و His204His المتماثل الزايكوت هو الأكثر أرتباطا بهذا المرض، ومع ذلك، يوصى بإجراء المزيد من الدراسات على عينة أكبر من المرضى لتأكيد هذه الأدلة.

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