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Article
Bacteremia in Ibn Al-Baladi hospital in Baghdad; Incidence etiology and antibiotic resistance of pathogens
تجرثم الدم في مستشفى ابن البلدي في بغداد:نسبة الحدوث, المسببات ومقاومة المضادات الحياتية لمسببات تجرثم الدم

Author: Faheema Jabbar Abu - Alhur فهيمة جبار ابوالهور
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2009 Volume: 6 Issue: 3 Pages: 549-552
Publisher: Baghdad University جامعة بغداد

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Abstract

60 cases of Bacteremia were documented at Ibn Al-Baladi hospital during 6 months (1-1-2002 to 1-7-2002), with an incidence of 5.2 were gram-negative organisms and most common one was Salmonella and Klebsiella. Incidence was significantly higher in male than female .Antimicrobial sensitivity tests revealed that isolated bacteria are with multiple drug resistance to commonly used antimicrobial agents. Salmonella showed high resistance to cephaloxin, co-trimoxazole and amoxicillin and also Klebsiella showed resistance to cephaloxin and amoxicillin.

تم تشخيص60 حالة اصابة بتجرثم الدم في مستشفى ابن البلدي للولادة والاطفال في بغداد خلال ستة اشهر(1-1-2002الى1-7-2002) وبنسبة حدوث 5.2 لكل 1000 حالة دخول وكان وقوع تجرثم الدم بسلبيات الغرام 90 % وكان من الجراثيم المعزولة الشائعة هي عصيات السالمونيلاSalmonella والكلبسيلة Klebsiella .كان وقوع تجرثم الدم عند الذكور اعلى من الاناث. كان تجرثم الدم اكثر ترددا في الاطفال بعمر اقل من سنة وقد اظهرت عتر السالمونيلا مقاومة للمضادات وخاصة السيفاليكسين,كو-ترايموكسازول و الاموكسيسيلين بينما اظهرت عتر الكلبسيلا مقاومة للسيفاليكسين,الامبسيلين والاموكسيسيلين.

Keywords

bacteremia --- pathogens --- antibiotic


Article
Comparative study on the Effect of Antibiotics and Non Antibiotics Therapy (Phytotherapy) on Some Bacteria Isolated from Urinary Tract Infection

Authors: Ikram Amin KhakiI --- Ban Auday Abdul sattar --- Zahraa Qasim Ali
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 1 Pages: 116-119
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSRACT:BACKGROUND:Phytotherapy is an alternative for antibiotics therapy in these days since resistance to many antibiotics used in treatment are increasing along with high cost of some antibiotics and side effect on patients. Punica granatum and Vaccinium macrocarpon have antimicrobial properties. this paper suggest that P. granatum and Vaccinium macrocarpon may be more effective than some antibiotics used in the treatment of some Gram negative urinary tract pathogens.OBJECTIVE:Punica granatum juice, cranberry juice and antibiotics were used to investigate the antimicrobial activity by using well diffusion method.MATERIALS & METHODS:A total of four different urinary tract causing pathogens collected from patients with urinary tract infection (UTI).RESULT:The result indicate that extract obtained from Punica granatum pericarp exhibited antimicrobial activity against all organisms almost similar to the effect of cranberry which is not grown in Iraq and that Punica granatum and Vaccinium macrocarpon are more effective than some antibiotics used in the treatment of urinary tract infection.CONCLUSION:The antibacterial activity of crude extract of P. granatum against urinary tract causing organisms is reported for the first time and it showed similarity with the effect of cranberry juice that are not found or imported to the local market. Further phytochemical elucidations are required to determine the nature of compound(s) responsible for the antibacterial effects. This study is generally considered an effective approach in the discovery of new antibacterial agents from P. granatum.


Article
THE PATTERN OF BACTERIAL PATHOGENS & THEIR ANTIBIOTICS SENSITIVITY AMONG PATIENTS WITH RESPIRATORY TRACT INFECTIONS

Author: Abdul-Munim N Mohammed د. عبد المنعم ناجي محمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 2 Pages: 181-186
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Knowing the bacterial pathogens and their antibiotic sensitivity is an important way of establishing a suitable guideline of treatment of infection.Objectives:To isolate bacterial pathogens from patients with respiratory tract infections (RTI), and to determine the antibiotic sensitivity of isolates.Methods:Sputum specimens were collected from 145 patients with RTI admitted to Al-Kindy Teaching Hospital from March 2011 to January 2012. Out of these, 88 (60.7%) patients (age rang 17-59 years) had an established bacterial etiology, and of these, 57 (64.8%) were males and 31 (35.2%) females. All isolates were diagnosed according to bacteriological and biochemical standard methods. For identified of antimicrobial susceptibility used from Kirby Bauer method according to (NCCLS).Results:Klebsiella species and Pseudomonas aeruginosa were the most common isolates among the Gram negative pathogens (26.2% and 11.7% respectively), followed by Escherichia coli and Proteus species, while Streptococcus pneumonia was the most common isolate among the Gram positive organisms, identified in (15.2%) followed by Staphylococcus aureus and Streptococcus pyogenes. High rates of resistance to Amoxicillin and Cephalothin were demonstrated by all bacteria, whereas most isolates were found to be highly sensitive to Amikacin, Ciprofloxacin and Tobramycin. In contrast, Cefotaxim, Tetracyciln, Gentamycin and Erythromicin were less effect against most of isolates.Conclusions:Klebsiella spp. was the most common pathogens, whereas Streptococcus pneumonia which ranks as second common pathogens from patients with RTI in the present study. Amikacin, Ciprofloxacin and Tobramycin were the most effect antibiotics in vitro against tested bacteria. Conversely, no or less effect of other antibiotic agents was obtained making them not to be considered the drugs of choice in treatment of patients with RTI.Keywords:Bacterial pathogens, Antibiotics resistance, Patients RTIs.


Article
Isolation and identification of Staphylococcus aureus, Listeria monocytogenes, E. coli O157:H7 and Salmonella specious from raw beef and lamb meat in Baghdad by PCR.
عزل و تشخيص جراثيم المكورات العنقودية الذهبية ,اللستيريا ,الاشريشيا القولونية والسالمونيلا من لحوم الابقار والأغنام النيئة في مدينة بغداد بواسطة تفاعل سلسلة البلمرة المتعدد

Author: Zena K. Khalil زينة قاسم خليل
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 3B Pages: 1891-1897
Publisher: Baghdad University جامعة بغداد

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Abstract

The study designed to determine the distribution of a major important food pathogens including Staphylococcus aureus , Listeria monocytogenes, E. coli O157:H7 and Salmonella spp from raw beef and lamb meat by using multiplex pcr . A total of 90 raw beef and lamb meat samples were collected from different butcher's shops in Al-Karkh side of Baghdad city and analyzed for the presence of these types of bacteria and their susceptibilities to some antibiotics was investigated ,the results showed that the prevalence of S. aureus (5.6%), L. monocytogenes (3%), E. coli O157:H7 (7.8 %) and Salmonella spp (5.6%) from the total samples .The result of the susceptibility test showed that S. aureus isolates were susceptible to Amikacin (80%) ,while L. monocytogenes isolates were susceptible to the most used antibiotics as following Amikacin, Erythromycin, Oxytetracycline, Nalidixic acid ,Cephalothin, Gentamycin, Ampicillin and Streptomycin (100%). E. coli O157:H7 isolates were susceptible to Nalidixic acid and Gentamycin (100%) and Salmonella spp isolates were susceptible to Nalidixic acid ,Cephalothin and Gentamycin (80%) .

تم تصميم الدراسة لتحديد مدى انتشار بعض الجراثيم الغذائية المهمة ومن ضمنها المكورات العنقودية الذهبية والليستيريا مونوسايتوجينز والاشريشيا القولونية والسالمونيلا من عينات لحوم الابقار والأغنام النيئة بطريقة تفاعل سلسلة البلمرة المتعدد. جمعت 90 عينة من لحوم الابقار والأغنام من محلات القصابة ضمن منطقة الكرخ من بغداد لتحديد وجود البكتيريا , كما تم اجراء فحص حساسيتها لبعض المضادات الحيوية واظهرت النتيجة تواجد بكتيريا المكورات العنقودية الذهبية بنسبة 5,6% والليستيريا مونوسايتوجينز 3% و والاشريشيا القولونية 7,8% والسالمونيلا 5,6% من مجموع العينات الكلية. نتائج فحص الحساسية سجلت حساسية المكورات العنقودية الذهبية للاميكاسين بنسبة 80% اما الليستيريا مونوسايتوجينز كانت حساسة لمعظم المضادات الحيوية والتي تضمنت (الاميكاسين,الارثرومايسين,الاوكسيتتراسايكلين, حامض الناليدكسيك, السيفالوثين, الجنتمايسين, الامبسلين والستربتومايسين) 100%, الاشريشيا القولونية كانت حساسة لحامض الناليدكسيك والجنتمايسين بنسبة 100% اما السالمونيلا فكانت حساسة لكل من حامض الناليدكسيك , السيفالوثين والجنتمايسين بنسبة 80% .


Article
Synthesis of Multi-Walled Carbon Nanotubes Decorated with Zinc Oxide Nanoparticles for Removal of Pathogenic Bacterial

Authors: Adawiya J. Haider --- Riyad H. AL- Anbari --- Hayder.A.Mohammed --- Duha.S. Ahmed
Journal: Engineering and Technology Journal مجلة الهندسة والتكنولوجيا ISSN: 16816900 24120758 Year: 2018 Volume: 36 Issue: 10 Part (A) Engineering Pages: 1075-1080
Publisher: University of Technology الجامعة التكنولوجية

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Abstract

A series of Zinc Oxide Nanoparticles (ZnONPs)-functionalized Multi-Walled Carbon Nanotubes (F-MWCNTs) Nano composites were developed as antibacterial. In this study, chemical oxidation of pristine MWCNTs were carried out with a mixture of strong acids (3H2SO4 98%:1 HNO3 65%).The F-MWCNTs were used as templates to prepare hybrid material like ZnONPs decorated F-MWCNTs. Pristine MWCNTs, F-MWCNTs and (ZnONPs/F-MWCNTs) Nano composites powder were investigated using Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Anti-bacterial activity has been carried out using standard agar dilution (plate count) method against Escherichia coli (E. coli). This study demonstrated that (ZnONPs/F-MWCNTs) Nano composite has a powerful bactericidal effect against Escherichia coli (E. coli) at concentration 0.5 mg/ml after 3 hr, which led to speculation that the combination of ZnONPs and F-MWCNTs altered their toxicity and improved antibacterial property of Nano composite.


Article
The Antimicrobial Activity of Lactobacillus Acidophilus Against Intestinal and Foodborne Pathogens
تأثير Lactobacillus acidophilus ضد مسببات الأمراض المعوية والتسمم الغذائي

Author: Luma Yousif Mehdi لمى يوسف مهدي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 3A Pages: 1189-1197
Publisher: Baghdad University جامعة بغداد

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Abstract

In this study, isolated of Lactobacillus acidophilus were evaluated for their anti-pathogenic bacterial activity. The antibacterial activity of the three isolated against intestinal and food borne pathogenic bacteria in vitro was determined by Well's Diffusion method, a total of three isolates of Lactobacillus acidophilus isolated from ten different brands of traditional yoghurts showed a various antibacterial activity against tested pathogenic bacterium, Cronobacter sakazakii isolated from stool samples was more sensitive to the inhibition(23mm)inhibtion zone than were Helicobacter pylori that isolated from stool samples (16mm) inhibtion zone and Clostridium perfringens that isolated from stool samples (15mm). These results may provide a basis for support therapies for the treatment of intestinal and foodborne pathogens.

في هذه الدراسة تم تقييم التأثير التثبيطي لعزلةLactobacillus acidophilus في مكافحة البكتريا المرضية المسببة للأمراض المعوية والتسمم الغذائي خارج الجسم الحي وبطريقة Well's Diffusion method, حيث تم عزل ثلاثة عزلات لLactobacillus acidophilus من عشر ماركات مختلفة من الزبادي التقليدي وأظهرت قابلية متنوعة لتثبيط نمو البكتريا المرضية ,حيث اظهرت Cronobacter sakazakii حساسية عالية للتثبيط 23mm))مقارنة ب Helicobacter pylori (16mm)وperfringens Clostridium ((15mm و قد توفر هذه النتائج أساسا لعلاجات مساعدة لعلاج الامراض المعوية والتسمم الغذائي.


Article
The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

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Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.


Article
Antibacterial Activity of Alcoholic and Aqueous Extracts of Agaricus bisporus Against Food Borne Bacterial Pathogens

Authors: Ebtehal Hamid --- Eman Mohammed Ali --- Alyaa Razooqi Hussein⃰
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 1 Pages: 111-114
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Two extracts of 50 mg.ml-1 Agaricus bisporus alcoholic and aqueous were used in order to study their effect against two pathogenic bacteria. Salmonella typhi and Staphylococcus aureus strains isolated from chicken and minced meat samples respectively, then identified according to biochemical tests. Antibacterial activity of Agaricus bisporus extracts (alcoholic and aqueous) was studied by agar well diffusion method. The results exhibited that aqueous mushroom extract has no effect against the two tested bacteria, while alcoholic extract of mushroom affect against the two tested bacteria and the effect against S. aureus was more than that against Salmonella typhi and the zone of inhibition was 15 mm and 9 mm respectively. The ethanol alcohol 96% and water were used as control. From this study, mushroom extracts especially ethanolic or alcoholic could be used to control the transmission of pathogenic bacteria from food


Article
Probiotic Activity of Lactobacillus spp. from Vaginal Specimens against Bacterial Pathogens

Authors: NibrasNazarMahmood --- Aya Ali Hameed
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2018 Volume: 26 Issue: 5 Pages: 335-346
Publisher: Babylon University جامعة بابل

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Abstract

Urogenital infections affect millions of people every year worldwide. The management of these diseases usually requires the use of antimicrobial agents. And more newly, the use of probiotic Lactic acid bacteria [LAB] cultures in the management of vaginal infections and other infections has been extensively studied. In this work 30 isolates of Lactobacillus spp. Were obtained from healthful Iraqwomen,s vagina. All the isolates were subjected to the cultural microscopically and biochemical examinations for the identification of species add to the identification by Vitek2 system [ANC card]. The results showed that half of isolates belongs to Lactobacillus casei and the other half belongs to Lactobacillus gasseri. agar well diffusion method and disc method are used to detect of bacteriocin production byLactobacillus.casei isolates against 4 species of Gram positive and Gram negative pathogenic bacteria which included:Corynebacteriumurealyticum, Staphylococcus aureus , Escherichia coli and Psudomonasaeuroginosa, which obtained from IMAMEIN KADHIMEN MEDICAL CITY . The results showed the Lactobacillus which isolated from vagina by well diffusion method was effective against pathogenic isolates more than the Lactobacillus isolated by blank disc method , the high inhibitory effect of Lactobacillus isolates by well give an inhibition zone reached to [26] mm , while the Lactobacillus by disc was lower with inhibition reached to [18]mm. The supernatant did not show any activity when was treated with NaOH and adjusted to pH 7. This indicates that the organic acid produced by the Lactobacillusisolates was may be actually responsible for the inhibition of the indicator bacteria .The result show that the Lactobacillusstrains could be considered as potential antimicrobial probiotic strains against some human vaginal pathogens and should be further studied for their human health benefits .

التهابات المجاري البولية تؤثر على الملايين الناس كل عام في جميع انحاء العالم, ان علاج الامراض عادة يتطلب استخدام عوامل مضادة للمكروبات, حديثا تم استخدام المعززات الحيوية لبكتريا حامض اللاكتيك لعلاج الاصابات المهبيلة و الاصابات الاخرى, تمت الدراسة (30) عزلة من بكتريا حامض اللاكتيك وتم الحصول عليها من مهبل النساء العراقيات السليمات و خضعت جميع العزلات للاختبارات المجهريهوالزرعيه و البايوكيمياىيةلغرض معرفة الانواع اضافه الى التشخيص بواسطة نظام الفايتك, النتاىج بينت ان نصف العزلات تابعه الى نوع الاول. استخدمت طريقة الانتشار بالحفر و طريقة الاقراص لغرض الكشف عن العزلات المنتجة للبكتريوسين والمستخدم لاختبار فعالية التثبيطية ضد اربعة انواع من البكتريا الموجبة والسالبة لصبغة كرام والتي تشمل[Corynebacteriumurealyticum, Staphylococcus aureus, Escherichia coli and Psudomonasaeuroginosa] وتم الحصول عليها من مختبرات مستشفى مدينة الامامين الكاظمين.ع. النتاىج اظهرت ان عزلات بكتريا حامض اللاكتيك بواسطة طريقة الانتشار بالحفر تاثيرها على عزلات البكتريا المرضية اكثر فعالية من طريقة الاقراص حيث يصل اعلى قطر لمنطقة التثبيط الى (26) ملم بينما اعلى قطر لمناطق التثبيط بطريقة الاقراص يصل الى (18) ملم .بينت النتاءج قابلية بكتريا حامض اللاكتيك على تثبيط البكتريا المرضية المسببه للالتهابات المهبلية و الاستفادة منها في علاج مثل تلك الحالات.


Article
Molecular detection of Chlamydia trachomatis infection among males with abnormal semen

Authors: Mays H. Ali --- Abdul Kareem A. Al-Kazaz
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 4B Pages: 2005-2011
Publisher: Baghdad University جامعة بغداد

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Abstract

Many studies revealed that Chlamydia trachomatis is the most prevalent bacteria “that cause sexually transmitted infections in the world.. Most of these infections are asymptomatic and there is a remarkable relationship between CT infections and male infertility. Therefore, “the present study is established to determine the effect of Chlamydia trachomatis infection on seminal fluid quality in males suffering from infertility compared with fertile males.. “Semen samples were collected from 63 infertile male and 13 fertile male as control group, attending the clinical laboratory for routine seminal fluid analysis. Seminal fluid was analyzed according to World Health Organization guidelines, the whole genomic DNA was extracted for molecular study. Real time PCR technique was used to specifically detect the presence of CT DNA in semen samples.” C. trachomatis was demonstrated in the semen samples of 11 (17.4%) infertile male and all control samples were negative. Infertile, infected males had semen samples that showed statistically significant differences in the mean of total sperm count, motility and morphology as compared with fertile uninfected control samples. These differences indicate that chlamydial infection of genital tract could negatively influence the quality of seminal fluid.”

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