research centers


Search results: Found 3

Listing 1 - 3 of 3
Sort by

Article
Platelet indices and their relations to platelet count in hypo-productive and hyper-destructive Thrombocytopenia

Authors: Khaleed Jumaa Khaleel --- Abeer Anwer Ahmed
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2014 Volume: 7 Issue: 2 Pages: 1952-1958
Publisher: Kerbala University جامعة كربلاء

Loading...
Loading...
Abstract

ackground: Thrombocytopenia is low platelets count which is either due to defective platelet production (hypo-productive thrombocytopenia) or due to increased platelet breakdown (hyper-destructive thrombocytopenia). Measurement of platelet counts alone do not explain the underlying patho-mechanism of thrombocytopenia.. Recent advances in automated blood cell analyzers facilitate the measurement of several blood cell parameters automatically such as, platelet indices including mean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit (PCT) and these parameters could provide some important clinical information.Aim of the study: to investigate whether platelet indices( MPV,PDW,PCT) could serve as diagnostic tools in the differential diagnosis of thrombocytopenia and, if there is any correlation between platelet count and platelets indices .Subjects and Methods: A total of 77 Iraqi patients (28 males and 49 females) with thrombocytopenia were subjected to the following tests:1. Complete blood count using Mindway haematologic autoanalyser.2. Peripheral blood film .3. Bone marrow aspirate with bone marrow trephine biopsy when indicated.A group of 50 healthy person (age & sex matched) were included as a controlResults: hyper-destructive thrombocytopenia (immune thrombocytopenia) representing 12.9% of total patients, while hypo-productive thrombocytopenia due to various causes representing 87.1% of total patients. The MPV, PDW are significantly higher in immune thrombocytopenia group as compared with thrombocytopenia of hypo-productive patho-mechanism and there were no correlations between platelets count and both MPV and PDW in hyper-destructive and hypo-productive thrombocytopenia but there was a direct correlation between platelet count and PCT .Conclusion: Platelet indices provide plenty of clinical information about the causes and patho-mechanism of thrombocytopenia and could be helpful tests to distinguish hyper-destructive thrombocytopenia from hypo-productive thrombocytopenia easily, so more interest for the use of these indices in differential diagnosis of thrombocytopenia is required.


Article
ESTIMATION OF PLATELET COUNT ON THE BASIS OF RED CELL: PLATELET RATIO
قياس عدد الصفيحات الدموية بالاعتماد على نسبة كريات الدم الحمر إلى صفيحات الدم

Author: Balsam F Abid بلسم فاضل عبد صالح
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2009 Volume: 7 Issue: 3 Pages: 40-45
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background: Modern haematology analyzers are able to produce platelet counts with great precision and accuracy. However, in certain cases these analyzers produce erroneous platelet results.Therefore, the estimation of platelet count from blood smears should be syste¬matic each time the automated count is erroneous. However, in comparison with the procedure for an automated count, the examination of a blood smear using a counting Neubauer chamber is a labor-intensive and therefore relatively expensive investigation. Objective: Verification of the reliability of the estimation technique of platelet count on the basis of red cell: platelet ratio.Material and Methods: In the period between January 2006 and March 2006 one hundred platelet counts were executed in the National Center for Haematological Diseases by two laboratory methods: an automa¬ted count using an impedance cell counter and then a manual method by reviewing microscopic blood smears. The number of platelets per 1000 erythrocytes was multiplied by the automated RBC (x106 cells/µl) to give an approxima¬te manual count (x103 cells/µl). Two-paired t-test was used for comparison of the two methods. Results: Platelet count using the manual method was as follow: the range was 100-499x103/µl, the mean count was 263.11±104.07 x103/µl, and the median was 247.5 x103/µl. using the automated method, platelet count ranged between 95-484 x103/µl, the mean was 258.43 x103/µl, and the median was 242.5 x103/µl. There was no significant difference in results of platelet count using both methods (P<0.05). Regression analyses gave the following equation by comparing the automated (y) to the manual method (x): y=0.9893x - 1.8621 (r= 0.966). The paired t-test showed no significant difference between the two methods (p<0.05). The ICC was equal to 0.988. The plot of the differences between the automated and manual values against their means showed that the difference mean was 2.116 with a standard deviation SD= 40.215. It was noticed that 93% of the differences were within the agreement limits (mean±2SD).Conclusion: Red blood cell:platelet ratio method requires only an accurate RBC count performed on a calibrated hematology analyzer to calculate platelet count. This method is precise, simple, and consumes less time than using a counting chamber, and therefore, potentially should supersede ordinary manual counting.Key words: red cell: platelet ratio, platelet count

خلفية الدراسة: إن الأجهزة التحليلية الحديثة المستعملة في مجال علم أمراض الدم تمتاز بقدرتها على إعطاء نتائج دقيقة ومحددة لعدد الصفيحات الدموية، لكن في بعض الحالات هذه الأجهزة تعطي نتائج مضللة وغير دقيقة لذلك يجب قياس عدد الصفيحات الدموية بالاعتماد على فحص مسحة الدم والذي يجب أن يكون من الفحوص المختبرية الروتينية خصوصا إذا كانت نتائج الأجهزة المختبرية التلقائية العمل مضللة، ولكن بالمقارنة مع هذه الأجهزة فإن الفحص المختبري لمسحة الدم باستخدام الطريقة التقليدية (حجرة نيوباور التعدادية) يتطلب الكثير من الوقت والجهد لذا فهو أكثر تكلفة اقتصاديا.هدف الدراسة: تقييم مدى صلاحية وموثوقية تقنية قياس عدد صفيحات الدم بالاعتماد على قياس نسبة كريات الدم الحمر إلى صفيحات الدم في مسحات الدم المصبوغة.المواد وطرق العمل: خلال الفترة من كانون الثاني إلى آذار 2006 تم قياس عدد الصفيحات الدموية لمائة عينة من الدم في المركز الوطني لأمراض الدم باستخدام طريقتين مختبريتين: الأولى بالاعتماد على جهاز قياس الخلايا التلقائي والثانية بالاعتماد على الطريقة اليدوية لقياس صفائح الدم باستخدام المجهر الضوئي وذلك بعد عد عدد الصفائح الموجودة في المسحة الدموية المصبوغة لكل ألف كرية دموية ومن ثم ضربها بعدد كريات الدم الحمر المقاسة باستخدام الأجهزة المختبرية التلقائية الحديثة. النتائـــج: كانت نتائج فحص مسحات الدم باستخدام الطريقة اليدوية كالآتي: تراوح عدد الصفائح الدموية للعينات المقاسة بين 100-499×103/مايكروليتر وكان معدل عددها 263,11± 104,07 ×103/مايكروليتر. في حين كانت النتائج باستخدام الأجهزة المختبرية لنفس العينات كما يلي: تراوحت أعداد الصفائح الدموية بين 95-484 ×103/مايكروليتر ومعدل عدد الصفائح كان 258,43 ±103,13 ×103/مايكروليتر. أظهرت النتائج عدم وجود فارق إحصائي معتد به بين عدد الصفائح الدموية المقاسة باستخدام الأجهزة المختبرية التلقائية وتلك المقاسة بالطريقة اليدوية بالأعتماد على نسبة كريات الدم الحمر إلى صفيحات الدم (P<0.05)الاستنتاج:قياس عدد الصفيحات الدموية بالاعتماد على نسبة كريات الدم الحمر إلى صفيحات الدم هي طريقة معتد بها وتحتاج فقط إلى قياس دقيق لعدد كريات الدم الحمراء باستخدام الأجهزة التحليلية المختبرية, حيث تساعد هذه الطريقة على قياس عدد الصفائح الدموية بدقة اكبر وفي نفس الوقت تستهلك جهدا ووقتا أقل مما تتطلبه الطريقة التقليدية لقياس عدد صفائح الدم باستخدام حجرة نيوباور التعدادية.مفتاح الكلمات: تعداد الصفيحات الدموية، كريات الدم الحمر، نسبة الصفيحات الدموية


Article
SIGNIFICANCE OF PLATELET INDICES IN PATIENTS WITH ACUTE ISCHEMIC STROKE

Authors: Waseem F Al-Tameemi وسيم فاضل محمد التميمي --- Ameer MM Ameen امير محمد امين
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 4 Pages: 383-389
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Background :Platelets play an important role in the development of intravascular thrombosis. Platelet size has been considered to reflect platelet activity. Platelet indices had been studied as an independent predictor of acute ischemic stroke (IS).Objectives:Assessing the relation of acute IS with different platelet indices.Methods:Fifty patients were enrolled: 25 of them had first acute IS (mean age 64 years, 12 (48%) were males) [group 1], while the rest 25 patients were those with more than one IS (mean age 68 years, 16 (64%) were males) [group 2] in comparison with the control group (20) subjects (mean age 57 years, 10 (50%) males). Work up included history and clinical examination in addition to brain CT scan and platelet indices which are: mean platelet count (MPC), mean platelet volume (MPV), platelet large cell ratio (P-LCR), and platelet distribution width (PDW) within first 24 hours of patient presentation.Results:The mean platelet count (MPC) was found to show significant difference between group 2 versus group 1 and control (P=0.012, P=0.023 respectively), while no statistically significant differences were reported with the other indices (MPV, PDW or P-LCR). Linear negative correlation was demonstrated between MPC and MPV, PDW and P-LCR in group 1, such correlation wasn't found in group 2.Conclusion:Mean platelet count (MPC) differs significantly in cases of multiple stokes in comparison with those having first stroke or the control group; therefore, the significance of this finding needs further studies.Keywords:Platelet indices, platelet count, mean platelet volume, platelet distribution width, platelet-large cell ratio, ischemic stroke.

Listing 1 - 3 of 3
Sort by
Narrow your search

Resource type

article (3)


Language

English (3)


Year
From To Submit

2014 (1)

2012 (1)

2009 (1)