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Article
Histopathological changes and metabolic gene polymorphism caused by aramite exposure in experimental rats
التغيرات النسيجية المرضية و التعدد والوراثي الناجمة عن التعرض للاراميت في الجرذان المختبرية

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Abstract

A total of 40 experiment rats ( Rattus norvegicus) were divided into three groups of 10 rats for G.1 normal rats; 15 rat for G.2 that exposed to aramite with food daily for 90 days (5 g aramite/1 kg of pellet diet) and 15 rat for G.3 that exposed to aramite with water daily for 90 days(5 g aramite dissolved in 1 litter tap water ). The result shows that the rats that were treated with aramite suffer from significant increased the activity and level of GPT; GOT ; ACP and ALP (P≤0.05) compared with control group , so the results fixed the histopathological changes in liver and intestine with clear mutation in GSTM1 gene in rats that were exposed to aramite with diet and water compared with the normal control rats.

مجموع 40 جرذ مختبري نوع Rattus. norvegicus قد قسمت إلى ثلاث مجاميع تمثلت ب10) G1 جرذان) كمجموعة سيطرة و15) G2 جرذان) عرضت للاراميت مع الغذاء لمدة 90 يوم بتركيز(5غم 1كغم عليقة ) و15) G3جرذان) عرضت للاراميت مع الماء لمدة 90 يوم بتركيز(5غم 1لتر ماء الحنفية) .أظهرت النتائج بحصول زيادة معنوية في مستوى خمائر GPT,GOT,ACP وALP في المجاميع المعاملة بالاراميت مقارنة مع مجموعة السيطرة .كذلك أظهرت الدراسة النسيجية حصول تغيرات نسيجية مرضية في الكبد والأمعاء وطفرات وراثية واضحة في جين GSTM1 في المجاميع المعاملة بالاراميت مع الغذاء والماء مقارنة مع مجموعة السيطرة.

Keywords

Aramite --- GSTM1 --- GSTT1 --- Polymorphism.


Article
Study of transferrin polymorphism in a population of Carp (Cyprinus carpio)

Author: T.A. Jaayidand N.M. Aziz
Journal: Marsh Bulletin مجلة الاهوار ISSN: 18169848 Year: 2009 Volume: 4 Issue: 2 Pages: 162-168
Publisher: Basrah University جامعة البصرة

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Abstract

This study was carried out at the college of agriculture, Basrah university. Fifty blood samples taken from carp (Cyprinus carpio) analyzed by Polyacrylamide gel electrophoresis (PAGE) under alkaline conditions at pH 9.8. The present study is aimed to provide the first insight into the genetic constitution of carp based upon analysis of Transferrin. Five genetic types of transferrin were revealed (Tf1Tf1, Tf2Tf2, Tf5Tf5, Tf3Tf2 and Tf1Tf5) in Carp controlled by four allelic genes of that locus (Tf1, Tf2, Tf3 and Tf5). These fractions are controlled by co dominant autosomal genes according to the Mendelian laws of inheritance. Differences in gene frequencies between alleles were observed. The gene frequencies of Tf1, Tf2, Tf3 and Tf5 were 0.15, 0.51, 0.29 and 0.05, respectively. The predominant genotype was Tf2Tf2 (44%) then Tf3Tf3 (22%). Transferrin was identified as highly polymorphic protein markers which is a result of importance for future genetic characterization of the carp .


Article
Analysis of Glutathione S-Transferase M1 and T1 Polymorphism in Samples of Iraqi Children with Autism

Authors: Nada Abed AL-Majeed AL-Ansari --- Esraa Mahmood Majeed Ahmed --- Haider Abdulmuhsin AL-Maliky
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2017 Volume: 16 Issue: 3 Pages: 28-38
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Aims: Is to find relationship between autism and absence of Glutathione-Stransferase (GSTs) genes GSTT1 and GSTM1, which are responsible for detoxification from the body. Methods: The present study includes the collection of (sixty) Iraqi children with a psychiatrist autism, the range of their age was 2-10 years. All blood samples were subjected to multiplex polymerase chain reaction (PCR) technique in laboratories of college of science (for Woman) Baghdad University for detection of the presence or absence of the gene encode for glutathione protein, and determination of the level of mercuryand lead in blood samples. Results: The results of the study showed that the male: female ratio in autistic children is (5.7:1). The percentage of children who have (GSTM1-null) genotype was 55.9% higher than the children 44.0% with (GSTM1-positive) genotype while the frequency of (GSTT1-null) genotype (11.8%) was lower than that of (GSTT1-positive) genotype (88.1%) in the tested cohort.The numbers of parents who have a positive GSTM1 genotype are lower than other groups while the number of parents who have a positive GSTT1 is higher than in other groups. The present study reveals that the presence of high concentrations of mercury in the blood samples for all children with autism compared with their families and with the normal value. Conclusions: It is concluded from the present study that the deletion or absence of GSTM1 genotype in autistic children is higher than presence. This indicates that GSTM1- null genotype is related to the ASD but not as a risk factor for autism. The highfrequency of GSTT1 positive genotype in autistic children and their parents is an indicator for a relationship between them. Further studies with large size of samples is needed to detect the activity of oxidative stress effect of enzymes encoded by alleles of GSTT1 genes.


Article
8.INTERLEUKIN-4 SINGLE NUCLEOTIDE POLYMORPHISM C-590T POLYMORPHISMS IN RELATION TO ASTHMA

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Abstract

Background:Single nucleotide polymorphisms in the promoter regions of genes encoding for some interleukins may associate with occurrence of asthma.Objective:To investigate the association of single nucleotide polymorphisms of interleukin-4 (IL-4) (C-590T) and asthma.Methods:Forty-five patients with asthma and 40 apparently healthy subjects (represent the control group) were enrolled in this study. Blood samples were collected from both patients and controls. DNA was extracted from blood samples and gene fragments corresponding to IL-4 C-590T were amplified with specific primers using conventional PCR technique. Results:The heterozygote genotypes of IL-4 C-590T (CT) showed significant association with asthma (OR = 3.922, 95% CI= 1.153-13.339, P = 0.028). Conclusion:These results suggest the significance of IL-4 C-590T polymorphism as a risk factor for asthma.Keywords:Asthma, interleukin-4, polymorphismCitation:Gaidan, Abbas AA, Hassan MA, Hashim HM. Interleukin-4 single nucleotide polymorphism C-590T polymorphisms in relation to asthma. Iraqi JMS. 2018; Vol. 16(1): 51-56. doi: 10.22578/IJMS.16.1.8

Keywords

Asthma --- interleukin-4 --- polymorphism


Article
Correlation between vitamin D receptor gene polymorphisms and levels of some hormones in Iraqi infertile women with polycystic ovary syndrome

Author: Sanaa Jasim Kadhim , Ismail A. Abdul-hassan
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 4 Pages: 104-113
Publisher: Baghdad University جامعة بغداد

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Abstract

The polycystic ovary syndrome (PCOS) is a disorder that characterized by hyperandrogenism, ovulatory dysfunction, and polycystic ovarian morphologic features. As defined by the diagnostic criteria of the National Institutes of Health (i.e., hyperandrogenism plus ovulatory dysfunction). The purpose of this study was to investigate the correlation between VDR gene polymorphisms and level of LH, FSH, TSH and Prolactin hormones .This study was carried out in the Institute of Genetic Engineering and Biotechnology for Postgraduate Studies - University of Baghdad through the period from November 2016 - August 2017, The PCOS patients were taken from the Kamal Al-Samarraee Infertility Treatment Hospital in Baghdad.Women with PCOS (n=50) and apparently healthy control group (n=50) , were enrolled . Genotyping of VDR gene (rs2228570) (rs7975232) , as well as (rs731236) SNPS between groups were determined by using Taqman genotyping assay . Hormonal analysis for LH, FSH, TSH and Prolactin was performed by using Automated Immune Assay (AIA). the results of the present study indicate that serum LH , FSH and TSH concentrations were unaffected by the studied SNPs of VDR gene within carriers of genotypes of rs2228570 , rs7975232 and rs731236 SNPs in VDR gene, while serum prolactin levels were significantly (p<0.05) higher in PCOS patients versus controls.

Keywords

PCOS --- VDR --- Polymorphism --- Infertile.


Article
Detection of AGT Gene Polymorphism in Patient with Hypertension in Mosul City

Author: Owayes M.H. Al- Hassani
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 64-69
Publisher: Baghdad University جامعة بغداد

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Abstract

Polymorphisms in the promoter region of the angiotensinogen (AGT) gene may affect AGT transcription and level of blood pressure. We determined the frequency of the AGT polymorphism in sample of Iraqi patients with primary hypertension. Using a molecular epidemiology approach, we also determined the relationship between primary hypertension and environmental-AGT polymorphism interactions of this study was to investigate the association between genetic polymorphisms of AGT M235T genes and Hypertension in Mosul city. Venous blood samples were collected from each subject in two separate test tubes: one was used for biochemical analysis. The other was collected in EDTA tube for DNA extraction. Genomic DNA was isolated from whole-blood samples of all the patients and control subjects. DNA concentration and purity were determined measuring by Bio drop and detected the optimum DNA concentration for PCR analysis. The quality of the DNA was determined using agarose gel electrophoresis stained with ethidium bromide, samples were stored at -20 °C until further use. The current study showed increase biochemical parameters in female patients compare with male patients. Regarding AGT/M235T gene polymorphism, In the present study, AGT/MT genotyping revealed that 47.5 % of patients, AGT/MM homozygous had 42.6 % and AGT/TT homozygous had 9.8 % in patients with Hypertension.

Keywords

Polymorphism --- AGT --- Hypertension --- DNA.


Article
ESTIMATION OF GENETIC VARIATIONS IN DIFFERENT TAXA IN BRASSICACEAE BY RAPD AND ISSR ANALYSIS
تقدير التغايرات الوراثية لمراتب تصنيفية مختلفة من العائلة الصليبية باستخدام تحليل RAPD و ISSR

Author: Huda Jasim M. Al-Tameme هدى جاسم محمد التميمي
Journal: Bulletin of the Iraq Natural History Museum مجلة متحف التاريخ الطبيعي العراقي ISSN: Print ISSN: 10178678, Online ISSN: 23119799 Year: 2018 Volume: 15 Issue: 1 Pages: 1-13
Publisher: Baghdad University جامعة بغداد

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Abstract

Twelve species from Brassicaceae family were studied using two different molecular techniques: RAPD and ISSR; both of these techniques were used to detect some molecular markers associated with the genotype identification. RAPD results, from using five random primers, revealed 241 amplified fragments, 62 of them were polymorphic (26%). ISSR results showed that out of seven primers, three (ISSR3, UBC807, UBC811) could not amplify the genomic DNA; other primers revealed 183 amplified fragments, 36 of them were polymorphic (20%). The Similarity evidence and dendrogram for the genetic distances of the incorporation between the two techniques showed that the highest similarity was 0.897 between the varieties red cabbage and red ornamental cabbage, meanwhile the lowest similarity index was between the varieties red radish and Green ornamental cabbage (0.169); thus these RAPD and ISSR markers have the possibility for the identification of species or varieties and the description of genetic variation within the varieties. Furthermore, it could be concluded that the Brassicaceae taxa have a suitable amount of genetic variance and a wide range in the genetic principle of the studied genotypes which can be used for output improvement.Keywords: Brassicaceae, Genetic similarity, ISSR, Polymorphism, RAPD.

درست اثنا عشر نوعاً من العائلة الصليبية باستخدام اثنين من التقنيات الجزيئية المختلفةRAPD وISSR ؛ إذ استخدمت كل من هذه التقنيات للكشف عن بعض الواسمات الجزيئية المرتبطة مع تحديد النمط الجيني اذ اظهرت نتائج تضاعف العشوائي المتعدد الاشكال لسلسلة الدنا RAPD، من استخدام خمسة بادئات عشوائية، تمييز241 حزمة مضخمة، 62 منها كانت متعددة الأشكال (26٪). أظهرت النتائج تكرار التسلسلات البسيطةISSR من أصل سبعة بادئات، ثلاثة (ISSR3، UBC807، UBC811) لم يظهر اي تضخيم للحامض النووي الجيني، وكشفت البادئات الأخرى 183 حزمة مضخمة، 36 منها كانت متعددة الأشكال (20٪) وتبين ان مؤشرات التشابه والمخطط التشجيري للمسافات الوراثية عند الجمع بين الطريقتين أن أعلى التشابه كان 0.897 بين أصناف الملفوف الأحمر والملفوف الزينة الأحمر، بينما ظهر أدنى مؤشر للتشابه بين أنواع الفجل الأحمر والملفوف الزينة الأخضر (0.169)؛ اي ان علامات RAPD وISSR لديها القدرة على تحديد الأنواع / الأصناف وتوصيف الاختلاف الجيني داخل الأصناف أيضا يمكن أن نستنتج أن انواع العائلة الصليبية لها كمية كافية من التنوع الوراثي ومجموعة واسعة في القاعدة الوراثية للتراكيب المستخدمة في الدراسة والتي يمكن استخدامها لتحسين المحاصيل.


Article
IDENTIFICATION OF LANCIFIELD SEROGROUP G STREPTOCOCCUS CANIS BY PCR-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS (PCR-RFLP) OF 16S RIBOSOMAL RNA GENE
( Streptococcus canis تحديد المكورات السبحية لجين (RFLP) و ال PCR بواسطة سلسلة تفاعل البلمره (Lancifield serogroup G 16S ribosomal RNA

Author: Abdulwahed Ahmed Hassan عبدالواحد احمد حسن
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2013 Volume: 12 Issue: 1 Pages: 208-227
Publisher: Basrah University جامعة البصرة

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Abstract

In this study S. canis and 12 various species and serogroups of streptococci including: S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G), S. uberis, S. parauberis, S. phocae, S. suis, S. equi subsp. equi, S. equi subsp. zooepidimicus, S. porcinus and S. pyogenes were used and identified reliably by PCR-restriction fragment length polymorphism analysis (PCR-RFLP) of 1.43 kb of 16S ribosomal RNA gene using universal oligonuclotide primers and subsequent digestion with the restriction endonucleases including RsaI, MspI and AvaII. The PCR-RFLP results showed that RsaI restriction RFLP pattern of S. canis appeared different with all streptococci species baring the S. equi subsp. equi and S. equi subsp. zooepidimicus. The MspI restriction RFLP pattern of S. canis could be differentiated from S. agalactiae, S. dysgalactiae subsp. dysgalactiae (serogroups C and L), S. dysgalactiae subsp. equisimilis (serogroups G), S. phocae, S. suis, S. porcinus and S. pyogenes. The AvaII restriction RFLP pattern of S. canis could be distinguished from S. dygalactiae subsp. dysgalactiae (serogroup C and L), S. dygalactiae subsp. equisimilis (serogroup G) S. parauberis, S. phocae and S. suis. In conclusion, PCR-RFLP method using restriction endonucleases RsaI, MspI and AvaII could be useful method for identification of S. canis from S. dysgalactiae subsp. dysgalactiae (serogroup C and L), S. dysgalactiae subsp. equisimilis (serogroup G) and other related streptococci species. It can be concluded that RFLP method might help to determine the prevalence of S. canis in animal and human infections .


Article
Association of The T45G Polymorphism of Adiponectin Gene with Polycystic Ovary Syndrome in Women of Babylon Province/ Iraq

Author: Hawraa Sabah Al-Musawi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 4 Pages: 1020 -1026
Publisher: Babylon University جامعة بابل

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Abstract

Polycystic Ovarian syndrome (PCOS) is being the most frequent cause of anovulatory infertility, Adiponectin is the most abundantadipocytokine and may play a role in the regulation of insulin sensitivity and IR in PCOS and count for 0.01% or 3–30 μg/ml of total plasma proteinthe adiponectin gene contains 3 exons spans 16 kb on chromosome3q27.The aim of the present study was to evaluate the genetic influence of the adiponectin gene polymorphisms in the development of PCOS among women of Babylon Province/ Iraq. sixty three women were studied ,and were classified into two groups of : first group consists of 32 women infected with polycystic ovaries syndrome, the second consists of 31 healthy women to detect the presence of T45G polymorphism within the gene. From all subjects a whole-blood sample was taken and was used for isolation of peripheral blood leukocytes. The adiponectin T45G polymorphism, located in exon 2, was genotyped by amplification of genomic DNA.The present study included study of the relationship of this gene with PCOS women in reproductive age, A statistically significant difference was observed in the frequency of TT,TG and GG genotypes between women with PCOS and controls .


Article
Association of T45G Genetic Polymorphism in The ADIPOQ Gene with Polycystic Ovary Syndrome Patients in Al-Najaf Province

Author: Karrar Saleem Zayed
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2017 Volume: 14 Issue: 3 Pages: 413 -421
Publisher: Babylon University جامعة بابل

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Abstract

This study was suggested to investigate genetic variation in ADIPOQ gene as a risk factor for polycystic ovary syndrome progression. Fifty-four Iraqi women with PCOS consulting at a Fertility and infertility treatment center were inducted into this study with a mean age was (24.23+5.19) years for these patients. Also, the study includes forty-eight normal healthy women collected randomly with mean age (23.81+6.01) years as a control group. The collection of blood samples carries out from PCOS and healthy control individuals for detection of biochemical diagnostic parameters in serum which include luteinizing hormone (LH) and follicles stimulating hormone (FSH), testosterone and adiponectin. Then, DNA was extracted from the blood of these two groups for a revelation of 45T/G genetic polymorphism in exon 2 of ADIPOQ gene for revealing of Polycystic Ovary Syndrome (PCOS) as a prognostic marker for this disease. The result of the current study appears that the serum adiponectin levels in PCOS decreased significantly when compared its level in control. In contrast, the level of LH and testosterone increased significantly in PCOs group than control. In regarding to genetic polymorphism 45(T/G) in exon 2 of ADIPOQ gene, the TT alleles polymorphism were increased significantly in control than PCOS patients while TG, GG alleles polymorphism were increased significantly in PCOS than control. The correlation between ADIPOQ polymorphism and level of adiponectin in serum demonstrated that the level of adiponectin is decreased significantly in presence of G allele in ADIPOQ gene in PCOS patients. This study concluded that the 45T/G polymorphism in ADIPOQ gene is highly elevated in PCOS patients especially polymorphism in GG alleles may be regarded as one of the main causes of PCOS occurrence. This result was proven the role of ADIPOQ gene in the pathogenesis of this syndrome.

Keywords

PCOS --- ADIPOQ --- 45T/G polymorphism

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