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Article
EVALUATION OF PROGESTERONE AND ESTRADIOL IN SERA AND TISSUE OF THYROID PATIENT

Author: Husam AK Ahmed حسام عبد الكريم احمد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2011 Volume: 9 Issue: 3 Pages: 206-208
Publisher: Al-Nahrain University جامعة النهرين

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BackgroundThyroid cancer is more common in subjects with blood group A and O. Estrogenic receptors in some cases of goiter were observed. Obesity is associated with increased risk of thyroid carcinoma possibly by mechanism of production of estrogenic steroids by adipose tissue. ObjectiveThis study was done to determine and compare the progesterone and estradiol in sera and tissue of female patients with various thyroid disorders.MethodsIn this prospective study, Serum and thyroid tissue homogenate were analyzed by measuring progesterone and estradiol in fifty normal healthy women volunteers as a control, in addition to ten patients with malignant thyroid nodules proved by histopathology, and thirty seven patients with benign thyroid nodules from March 2008 to August 2009 in Al-Kadhimiya Teaching Hospital and Al-Dirgham private hospital in Baghdad.ResultsSerum estradiol and progesterone levels for both malignant and benign thyroid nodules patients were less than noticed in healthy control, while tissue estradiol and progesterone levels in malignant thyroid tumor were significantly higher than those in benign thyroid nodules.ConclusionTissue estradiol and progesterone levels can be used in the diagnosis and differentiation between malignant and benign thyroid nodules.Key word: progesterone, estradiol, Thyroid nodules


Article
SERUM ESTRADIOL AND PROGESTERONE IN IMMATURE MALE AND FEMALE WATER BUFFALO(BUBALUS BUBALIS) IN MARSHES OF IRAQ
مستوي الاسترادايول والبروجستيرون في مصل ذكور وإناث الجاموس في مناطق اهوار جنوب العراق

Author: Khalid Al-Fartosi خالد كاطع الفرطوسي
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: 18138497 Year: 2012 Volume: 11 Issue: 1 Pages: 116-121
Publisher: Basrah University جامعة البصرة

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The present study aimed to determine some of reproductive values of water buffaloes in marshes of Iraq which may be used as a primary values for assaying the reproduction and production of water buffaloes. The study performed on immature male and female water buffaloes. Estradiol and progesterone hormones were determined. The results showed effect of sex on the levels of estradiol and progesterone of male and female buffaloes. The age was effective in estradiol and progesterone levels of female buffaloes, while it was no effective on estradiol and progesterone levesl of male water buffaloes.


Article
BLOOD PROGESTERONE AND ESTROGEN HORMONES LEVEL DURING PREGNANCY AND AFTER BIRTH IN IRAQI SHEEP AND GOAT
قياس مستوى هورموني البروجسترون والاستروجين خلال الحمل وبعد الولادة

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Serum progesterone and estrogen concentrations were investigated during pregnancy and few days after birth. Blood samples were collected twice / month from 24 numbered animals ( 12ewes and 12 does) . serum was isolated and kept under -20C untill hormonal analysis. Enzyme - Linked Immunosorbent assay (ELISA) using (ELISA Reader Dona 3200). Progesterone concentrations of pregnant ewes and does were showed steadily increased to reach 24.9+ 2.5 ng/ml and 30.34+ 2.3ng/ml in ewes and does, during 4th month and declined to 0.6 and 2.5 ng/ ml after birth in ewes and does, respectively. Estrogen hormone levels in ewes were increased significantly during pregnancy to maximum 98.7+4.3ng/ml by 5th month and sharply declined to 4.1+0.06 after birth. While in does estrogen level increased significantly and steadily to 1150.6+ 6.23 pg/ml during last month of pregnancy and to 5.9+ 0.4 pg/ml after birth. The present work indicated levels of progesterone hormone increased during 1st and 2nd months in ewes and does, while the significant increased from 3rd month on . Estrogen reach maximum concentrations during last month in doe Which higher about 11 times than that of ewes .It is useful means to diagnosis pregnancy of ewes and does by hormonal methods after mating 20-30 days.

يهدف البحث لقياس مستوى هرموني الحمل البروجسترون والاستروجين وبعد الولادة. جمع الدم من 24 رأس (12 نعجة و12 معزة )مرتين في ألشهر من كل حيوان . فصل وحفظ مصل الدم وتم قياس الهرمونات بطريقة Enzyme –linked immunosorbent assay(ELISA) وباستخدام (ELISA Reader Dona 3200) أعلى مستوى لهرمون البروجسترون كان في الشهر الرابع من الحمل 24.9+2.53 و 30.34+2.23 نانوعرام /مل في الأغنام والمعز وينخفض بصورة حادة بعد الولادة الى 0.6 و 2.5 نانوغرام /مل في الأغنام والماعز على التوالي . أما هرمون الاستروجبن في الأغنام والماعز فكان يزداد بصورة تصاعدية ومعنوية خلال أشهر الحمل ليصل إلى أعلى مستوى له في الأغنام 98.65+ 2.4 والماعز 1150.6+ 6.23 بيكوغرام / مل خلال الشهر الأخير من الحمل . بعد الولادة ينخفض إلى اقل مستوى فيصل 4.1 للأغنام و 5.9 للماعز. نستنتج من هذه الدراسة بان هرمون البروجسترون يرتفع خلال الشهر الثاني والثالث ليصل إلى أعلى مستوى للأغنام والماعز خلال الشهر الرابع للحمل .هرمون الاستروجين في الماعز يزداد خلال الحمل تصاعديا ويصل أعلى مستوى قبل الولادة و يصل مستو الهرمون 11 مرة أكثر مما في النعاج . من المفيد تشخيص الحمل في الأغنام والماعز بقياس هرمونات البروجستون والاستروجين بعد التسفيد أو التلقيح بـ 20-30 يوم. خلال أشهر الحمل

Keywords

: progesterone --- ELISA --- pregnancy


Article
Efficacy and acceptability of depo-medroxy progesterone acetate injection among Iraqi women
كفاءة وقبول عقار ديبومدروكسي البروجسترون العراقيات كوسيلة لمنع الحمل لدى النساء

Author: Ali M. Murad د. علي محمد مراد
Journal: IRAQI JOURNALOF COMMUNITY MEDICINE المجلة العراقية لطب المجتمع ISSN: 16845382 Year: 2007 Volume: 20 Issue: 1 Pages: 73-76
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Objective: To determine the efficacy and acceptability of Depo-medroxy progesterone (Depo-Provera ) among the women using this method of contraception at Al-Yarmouk Teaching Hospital.Method: A preliminary cross sectional study and questionnaire analysis of 100 Iraqi women who had Depo-Provera as a method of contraception at the contraception clinic of Al Yarmouk teaching hospital over 6 months. Results: The mean age of the women was 31 years, and the mean parity was 6 There was no pregnancy reported during the period of use of contraceptive method which ranged from 3 months to 5 years. The side effects were mainly irregular spotting (68%), amenorrhea 9%, continuous bleeding pervaginum 5% , and menorrhagia 3% .The rest reported normal menstrual pattern .Irregular spotting was common in women who had used the method for less than 2 years while amenorrhea was the most common menstrual abnormality after 3 years of use. The other include weight gain, loss of hair, abdominal pain and backache. The side effects were not acceptable to 5% of the women and they tried other method of contraception. 20% of women get pregnant between 6 months to 2 years after stopping the injections.Conclusion: Depo-Provera is very effective form of contraception in our community. While a few of women 5% would try other methods if they were not happy with the side effects as long as desired prevention of pregnancy was guaranteed. Depo-Provera injection contraception. Key words: depo-medroxy progesterone acetate

الملخص :الأهداف : تقدير الكفاءة والقبول لعقار ديبومدروكسي البروجسترون بين السيدات المستخدمات لهذة الوسيلة في مستشفى اليرموك التعليمي . طريقة البحث : أجريت الدراسة الأولية بأستخدام الأستبيان على 100 سيدة عراقية يراجعن في عيادة تنظيم الأسرة في مستشفى اليرموك التعليمي على مدار ستة أشهر . النتائج : كان متوسط العمر هو 31 سنة وعدد مرات الولادة هو 6 . ولم تسجل حالة حمل واحدة خلال فترة الأستخدام التي تراوحت بين ثلاثة أشهر وخمس سنوات . كانت الأعراض الجانبية الأساسية كالتالي نزف بسيط غير منتظم (68%) امتناع الطمث (9%) نزف مهبلي مستمر (5%) والنزف الشديد (3%) وسجلن بقية السيدات نمطاً طمثياً عادياً . كان النزف البسيط غير المنتظم أكثر شيوعاً في السيدات اللآتي استخدمن الوسيلة لمدة أقل من عامين بينما الشكاوي الأخرى هي زيادة الوزن ، تساقط الشعر والآم البطن والظهر . ولم تتقبل 5% من السيدات هذه الأعراض وحاولن استخدام طريقة أخرى لمنع الحمل . وحملن حوالي 20% من السيدات بعد توقف استخدامهن للحقن في فترات تتراوح بين ستة أشهر وعامين. الأستنتاج : لايزال عقار الديبو بروفيرا مفضلاً كوسيلة لمنع الحمل في مجتمعنا .


Article
down -regulation of progesterone hormone in recurrent pregnancy loss

Authors: Amal Hussain Salman*3 (PhD Microbiology) --- Asmaa’ Baqer Al-Obaidi*2 --- Alaa Gani Hussain*1 (FICMS) (MSc Microbiology)
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2008 Volume: 50 Issue: 1 Pages: 50-55
Publisher: Baghdad University جامعة بغداد

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Abstract

Background:Progesterone hormone is important in the preparation for and in maintaining of pregnancythrough different mechanisms but mainly by shifting the balance from Th1 to Th2 mediatedimmunity to avoid rejection of the fetus; in this study we tried to find out whether there is arelation between the level of the expression of progesterone and its stimulant HCG hormone andrecurrent pregnancy loss (RPL).Patients and Methods:Immunohistochemistry technique was performed to detect and determine the expression ofprogesterone and HCG hormones using paraffin embedded sections of curate samples obtainedfrom 40 women, who where divided into three groups: 24 women with RPL, 10 women withabortion for the first time, and 6 women with induced abortion.Fac Med Baghdad200Results:The levels of the expression of both progesterone and HCG hormones were found to besignificantly down-regulated in the first group as compared with the second group (p=0.00), andthe third group (p=0.00), with a highly significant positive correlation between these twoparameters (r=0.866, p<0.01).Conclusion:Low expression of progesterone in women with RPL could raise the possibility ofunderlying immuno-endocrine pathology responsible for this recurrent loss due to the importantrole of progesterone as an immunosuppressive hormone help in maintaining pregnancy.Key wards: Progesterone, HCG, RPL

Keywords

Progesterone --- HCG --- RPL.


Article
The Effects of Estradiol and Progesterone Therapy on Markers Expression of Vimentin and Desmin in Rat Uterine Tube. Immunohistochemical Study
تأثيرات علاج الاسترادايول والبروجستيرون على التعبير عن واسمات الفايمنتين والدسمين في القناة الرحمية للجرذ. دراسة كيميانسجية مناعية

Authors: Hussein Abbas Jarullah --- Haider AR Ja'afar --- May F. Alhabib
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 2 Pages: 399-408
Publisher: Babylon University جامعة بابل

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Background: Estrogen and progesterone are ovarian hormones that can be used as oral contraceptive pills (in combined formula) in addition to their use ashormonal replacement therapy in menopause.Methods: A sample of 30 animals having an estrous cycle of 4 days period was used. The animals were divided into 2 main groups; a control group (6 rats) and treated group which further divided into 3 subgroups; T I, T II and T III (8 rats for each) according to the dose of Estradiol which was given in three different dosages (1, 4 and10 µg/day) for a period of two successive estrous cycles (i.e. 8 days).The Progesterone hormone was given in a dose of 4 mg/kg body weight, for all the 3 subgroups, on the third and fourth days of the two successive estrous cycles. Immunohistochemical study was done through the applicationof Vimentin and Desmin markers.Staining procedure: (using Labelled Strept-Avidin Biotin LSAB™+/HRP kit, code number K0697 detection system). Aperio Positive Pixel Count Algorithm software (modified) was employed, in the studyResults:The demonstration of desmin was apparent mainly in the smooth muscle cells of the oviductal wall and highest immunoreactivity was found to be in the proestrous phase.In the treated group; high decline in the staining reactivity was found, especially in T I group.The demonstration of vimentin reaction was evident mainly in the lamina propria stromal cells and the tunica muscularis of the rat oviduct. The immunoreactivity was found to be high in the proestrous phase.In the treated group; profound reduction in the staining reactivity of the lamina propria and smooth muscle cells was found,especially in T Igroup and little immunoreactivity for vimentin receptors in treated groups. This due to effect of combine hormonal therapy(estrogen and progesterone) on alternation immunogenic configuration of vimenin and desmin intermediate filaments of rat oviduct cells.Conclusion:The combined therapy reveals thatdesmin and vimentin are essential for cell integrity and apply as indicator for metaplastic activity of cells.

يعتبر الاستروجين والبروجسترون من الهرمونات المبيضية والتي يمكن أن تستخدم، بصيغتها المشتركةِ، كحبوب مانعة للحمل عن طريق الفم بجانب استخدامها كعلاج بالهرمونات البديلة أثناء وبعد سن اليأس.اظهرت التفاعلية المناعية للفايمنتين وضوحا وبشكل رئيسي في خلايا انسجة الصفيحة الاصيلة و الغلالة العضلية للانبوب الرحمي للجرذ. وكانت هذه التفاعلية المناعية عالية في طور مقدمات الوداق. اما في مجموعة المعالجة ، فقد وجد انخفاض عميق في التفاعلية التلونية للصفيحة الاصيلة و خلايا العضلات الملساء ، وخاصة في مجموعة T I . كانت مظاهر الدسمين تظهر بشكل رئيسي في خلايا العضلات الملساء في جدار الانبوب الرحمي ووجدت اعلى تفاعلية مناعية في طور مقدمات الوداق. اما في مجموعة المعالجة؛ فقد وجد انخفاض عالي في التفاعلية التلونية، وخاصة في T I.لقد كشف العلاج المشترك باستخدام الاسترادايول والبروجستيرون ان الدسمين والفايمنتين ضروريان لسلامة الخلية كما ويمكن استخدامهما كمؤشر للنشاط الحؤولي (الاستحالي) للخلايا.في هذه الدراسة استخدم برنامج (Aperio positive pixel count algorithm) المعدل لغرض تقييم وتحديد توزيع الدسمين والفايمنتين في منطقة الانبورة للانبوب الرحمي للجرذ.

Keywords

Vimentin --- Desmin --- Estradiol --- progesterone.


Article
Effect of Metronidazole drug in the Levels of some Pregnancy Hormones during Blastocyst Implantation in Rat Uterus
تاثير عقار الميترونيدازول في مستويات في بعض هرمونات الحمل خلال غرس الكيسة الاريمية في رحم الجرذ

Authors: Kadhim M. Haddao --- Akram Y. Yasear --- Hussain A. Abdullatif
Journal: journal of kerbala university مجلة جامعة كربلاء ISSN: 18130410 Year: 2014 Volume: 12 Issue: 3 Pages: 9-15
Publisher: Kerbala University جامعة كربلاء

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Abstract

This study aimed to determine the effect of metronidazole (MTZ) drug on the levels of progesterone and estrogen hormones during blastocyst implantation in the pregnant rat uterus during the implantation period. Understanding the roles of the variety of pregnancy hormones in uterine receptivity for implantation is essential to enhancing reproductive health and fertility in humans and domestic animals. Forty eight female rats of confirmed pregnancy have been used ,divided into two treated groups received oral dosage commercial drug, 9 mg / 200 gm of life body weight as treatment dose of drug, 18 mg as double the treatment dose and 27 mg as the triple treatment dose. The results of this study revealed that taking treatment dose and double dose of the drug for 7 dpc group of pregnant rats cause decrease the level value of estrogen hormone in blood serum but this decrease didn’t reach significant level p>0.05. It was significant p<0.05 at the triple dose, and it is significant p<0.05 inversely for 9 dpc period, It means that the estrogen hormone level was decreased when the drug dose was increased were there were significant decrease p<0.05 in the value of progesterone level of blood serum at treatment dose, double and triple dose of the drg. There was significant affect p<0.05 for the mean of progesterone level in blood serum of the treated animal.

هدفت الدراسة الحالية إلى تبيان اثر عقار الميترونيدازول التجاري على معدلات قيم مستويات هرموني البروجيستيرون والاستروجين خلال غرس الأجنة في رحم الجرذ في اليومين السابع والتاسع من الحمل، حيث ان تقبل الرحم لانغراس الكيسة الاريمية في هذين اليومين يعتمد على هذين الهرمونين وفهم ادوارهما وغيرهما في تقبل الرحم لانغراس الكيسة الاريمية ضروري إلى تَحسين صحة الحوامل والخصوبة في البشر والحيوانات الأليفة. تم استخدام 48 جرذاً أنثى جرعت فمويا 9 ملغم من العقار / 200 غم من وزن الحيوان الحي و 18 ملغم و 27 ملغم يوميا ولمدة سبعة أيام وتسعة أيام ومن اليوم الأول من تأكيد الحمل. اجريت الدراسة في 2012 بوحدة ابحاث الرزازة وغربي الفرات - جامعة كربلاء. أظهرت النتائج الاحصائية أن تجريع عقار الميترونيدازول بجرعة علاجية وضعفها أدى إلى انخفاض في مستوى هرمون الاستروجين في مصل دم الجرذان الحوامل الا انه لم يصل إلى مستوى المعنوية p>0.05 في حين أن تجريعها بثلاثة أضعاف الجرعة قد أدى إلى انخفاض معنوي p<0.05 في مستوى الهرمون. وعلاقة الارتباط r غير معنوية p>0.05 عكسية لفترة 7 أيام ومعنوية p<0.05 عكسية لفترة 9 أيام. وهذا يعني ان مستوى الاستروجين قد انخفض عند زيادة جرعة الميترونيدازول الى ثلاثة اضعاف الجرعة العلاجية . وان تجريع العقار بجرعة علاجية وضعفها وثلاثة اضعافها قد أدى إلى انخفاض معنويp<0.05 في قيمة مستوى هرمون البروجيستيرون بمصل الدم وان علاقة الارتباط معنوية p<0.05 عكسية


Article
Salivary progesterone as predictors of spontaneous preterm birth in comparism with cervical length

Author: Shatha sami Hussein
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 3 Pages: 64-68
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Preterm labour still one of important causes of neonatal mortality and morbidity. Many researches on preterm labor were conducted in order to decrease the incidence, prevent complications, and improve survival rate for infants. Objective: To assess if salivary progesterone can be used sa a predictive marker for preterm labour and to compared it to the use of cervical length measurement. Study design: This Prospective observational study Setting: The study was performed at AL-Yarmouk Teaching Hospital at the Department of Obstetrics and Gynecology (Baghdad – Iraq) for the period from February 2015 to February 2016 Patients and methods: The study involved 60 pregnant women between 28 weeks-34weeks gestation were divided into two groups; group A 30 patients with uterine contraction or history of previous preterm .group B 30 patient as control, cervical length of all patients was measured by transvaginal ultrasound ,saliva sample(washings) also collected and the level of progesterone in the saliva was measured by ELISA and the results were compared. Results: statistical analysis of data showed that there was a significant relationship between salivary progesterone levels and preterm birth Sensitivity, specificity , predictive values (positive and negative) of progesterone were 96.7,90.0,90.6,96.4 respectively Conclusion: salivary progesterone can be used as a predictor of preterm delivery in high risk group.


Article
6.EFFECT OF PROGESTERONE IN LOWERING MATERNAL PLASMA CORTICOTROPIN-RELEASING HORMONE IN PATIENTS WITH PRETERM LABOR

Authors: Enas M. Yaseen ايناس محمد ياسين --- Athraa Fadhil Abd ايثار فاضل عبد
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2019 Volume: 17 Issue: 1 Pages: 32-42
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Preterm labor is a major obstetrics problem because it associated with high morbidity and mortality to the born baby. Many studies were done to study different aspects of its risk factors, diagnosis and treatment to decrease its incidence, bad sequel to the fetus and recurrence.Objective:To evaluate the role of progesterone in lowering CRH level in plasma of patient with preterm labor.Methods:This study is a case control study. Forty-five pregnant women with preterm labor were included in the study and basal plasma Corticotropin releasing hormone (CRH) level was done for each patient. All patients were given oral tocolytic drug, with an initial bolus of 20 mg Nifedipine followed by 10 mg three times daily. They received betamethasone injection (12 mg) 24 hours after hospitalization. Then after stabilization of each patient with random selection, 25 patients were included in group (1) and were given progesterone injection. Twenty patients were included in group (2) or control group because no progesterone injection was given to them. After 24 hours of admission to the hospital, plasma CRH level was measured for both groups with evaluation of the outcome of each patient with preterm labor.Results:Progesterone is more effective in lowering CRH level in patients with preterm labor. In study of 45 cases of preterm labor, the mean CRH level of group (1) decreased from 33.41 ng/ml to 22.12 ng/ml, while the mean level of it in group (2) increased from 27.44 ng/ml to 28.54 ng/ml. Conclusion:Progesterone treatment is effective in lowering CRH level in patients with preterm labor. This would have a positive effect in prolonging the pregnancy period in these patients.Keywords:Preterm labor, progesterone, CRHCitation;Yaseen EM, Abd AF. Effect of progesterone in lowering maternal plasma corticotropin-releasing hormone in patients with preterm labor. Iraqi JMS. 2019; 17(1): 32-42. doi: 10.22578/IJMS.17.1.6

Keywords

Preterm labor --- progesterone --- CRH


Article
Enzyme linked immunosorbent assay (ELSA) investigation for Human Kisspeptin and progesterone Levels in female having regular menstrual cycle

Author: Ahsan K. Abbas احسان كاظم عباس
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2011 Volume: 53 Issue: 2 Pages: 211-215
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Kisspeptin the product of the gene Kiss1 is a G-protein coupled receptor legend for GPR54. Kiss1 was originally identified as a human metastasis suppressor gene that has the ability to suppress melanoma and breast cancer metastasis. It is recently become clear that kisspeptin-GPR54 signaling has an important role in initiating GnRH secretion at puberty, the extent of which is an area of ongoing research.
Objective: The present study was designed to determine the change of plasma kisspeptin levels during the menstrual cycle
Materials & Methods: A total of 20 women from friends and relatives pool were involved in this study. Selected from frinds and relative .They were selected after measuring their progesterone level on day 21 of menstrual cycle (excluding women who have anovulate menstrual cycle; low level of progesterone) , and then on 1 -5 day of the next menstrual cycle. All measurements were done in Teaching laboratories in Baghdad Teaching Hospital in Baghdad/Iraq. Five mls of blood withdrawn from each selected women by veinpuncture of selected women. ELISA technique was used for the measurement of serum kisspeptin -1and progesterone level. Data were expressed as a mean ±SD. Results were evaluated using the student t-test for paired data. Conventional methods were used for the correlation and regression analyses.
Results: Results obtained showed that the levels of serum kisspeptin and progesterone were significantly higher on day 21of menstrual cycle than during menstruation period with p<0.01, also it was found a significant positive correlation of kisspeptin level with progesterone level (r= 0.77 ,p<0.01).
Conclusion: The results determine the role of kisspeptin on ovulation, and give a possibility for its beneficial manipulation of human fertility.

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