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Article
Aflatoxin B1-Induced Kidney Damage in Rats

Authors: Hayder Hammadi Abdul-Ameer --- Akram Abood Jaffar --- May Fadhil Majid Al-Habib
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 1 Pages: 147-150
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Aflatoxin B1 (AFB1) is a widely distributed mycotoxin in nature. Several investigations have shown its biological effects on different organs and in different animal species. However, the effects of AFB1 on the rat kidney have not been much elucidated histologically. Objective: This study aims to demonstrate the effects of AFB1 contaminated diet on the rat kidney from the histological and morphometric aspects. Method: Twelve mature albino rats were divided equally into a control group fed with usual diet and a treated group which was daily fed with diet contaminated with 20 mg AFB1/kg of body weight for 30 days. Semithin sections from renal cortex were stained with methylene blue and examined by light microscopy. Corpuscular changes were also detected morphometrically in terms of the ratio between the area of Bowman’s capsule and the area of its contained glomerulus (B/G ratio). Results: The treated group showed a marked increase in body weight. Histologically, there was evidence of acute tubular necrosis and increase in urinary space. Morphometrically, there was a diffuse significant increase in the B/G ratio compared to the control. Conclusion: Gain in weight can be attributed to fluid retention that accompanies the ensuing renal damage. The dietary dose of AFB1 (20 mg/kg of body weight) for 30 days was sufficient to produce acute tubular necrosis. The corpuscular changes indicated by the increase in the B/G ratio can be attributed to compensatory hypertrophy.

Keywords

Aflatoxin --- Kidney --- Rat --- Morphometry


Article
Glycoconjugates cytochemistry in teeth development in normal and hydrocortisone treated rats (experimental study)

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Abstract

Background: This study was designed to investigate the expression of glycoconjugates in rat teeth germs at various stages and to evaluate the effect of hydrocortisone on the formation and development of dentition of the rat histochemically.Materials and methods: Foetuses of albino rats were divided into tow main groups, the control group were injected with one ml distilled water (12-18day of gestation) and experimental group were injected with one ml hydrocortisone sodium succinat (12-18day of gestation) then specimens were stained with H & E and lectinsResults: The developing teeth are effected by the hydrocortisone sodium succinate drug at all stages of tooth development, lectins used through this study can serve as a marker for tooth development process, sugar residues (Glc NAC, GLC, Gal, Gal NAC) are carbohydrate components of predentin of the control group while less residus were found in the treated groups and they were to a less extent at the inner dental BM (basement membrane).Conclusion: This drug affect rat teeth development, the abnormalities are confirmed by the stains H and E, and lectins

Keywords

Hydrocortisone --- rat --- lectins


Article
Effects of Prenatal metallic mercury vapor exposure on the developing teeth of rats

Author: Ghada M. Mustafa B.D.S . M.Sc.* د. غادة مصطفى
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2006 Volume: 3 Issue: 2 Pages: 197-201
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

This Study represents an attempt to investigate histologically the effect of metallicmercury vapor on the developing rat teeth after inhalation by pregnant rats .In this study albino – wistar rats were used and divided into control andexperimental groups.The control group consisted of 8 pregnant rats, and the experimental groupconsisted of 12 pregnant rats. Then 20 born rats from the control group and 20 bornrats from the experimental group were selected to be sacrificed at the ages of (3&13)days.The specimens were prepared for processing and staining with haematoxylin andeosin, and examined under light microscope.Results showed no histological abnormalities in the dental tissue of theexperimental group when compared to that of the control group .This Study represents an attempt to investigate histologically the effect of metallicmercury vapor on the developing rat teeth after inhalation by pregnant rats .In this study albino – wistar rats were used and divided into control andexperimental groups.The control group consisted of 8 pregnant rats, and the experimental groupconsisted of 12 pregnant rats. Then 20 born rats from the control group and 20 bornrats from the experimental group were selected to be sacrificed at the ages of (3&13)days.The specimens were prepared for processing and staining with haematoxylin andeosin, and examined under light microscope.Results showed no histological abnormalities in the dental tissue of theexperimental group when compared to that of the control group .


Article
The physiological performance of offspring nursing from dams treated with different levels of sodium tungstate
الاداء الفسيولوجي للمواليد المرضعة من امهات معاملة بمستويات مختلفة من تنكستات الصوديوم

Author: Suha A. Rasheed سهى عبد الكريم رشيد
Journal: The Iraqi Journal of Veterinary Medicine المجلة الطبية البيطرية العراقية ISSN: 16095693 Year: 2012 Volume: 36 Issue: spcial issue(1) Pages: 43-49
Publisher: Baghdad University جامعة بغداد

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Abstract

This study aimed to recognize the different effects of administration of sodium tungstate in developmental indices of the offspring till weaning. Four groups of Albino Wistar pregnant female rats were used, the first one was control while the remaining three groups intubated with Na- tungstate 75, 150 and 300 mg/ kg/ day respectively since the first day of parturition throughout 21 days. Results referred to an elevation in weight gain of rat dams treated with 75 mg/ kg while weight gain of offspring elevated in both doses of 75 and 300 mg/ kg throughout 21 days, teeth and eye orifice were observed in doses of 75 and 300 mg/ kg faster than control while the mean of ovary weight elevated in both 75 and 300 mg/ kg however the dose 300 mg/ kg revealed the less value of testes weight. The conclusion of present study is that Na- tungstate has positive effects on developmental indices in offspring when administered through nursing until weaning.

صممت الدراسة الحالية للتعرف على التأثيرات المختلفة لتنكستات الصوديوم في النواحي التطورية لفترة ما بعد الولادة وعن طريق الرضاعة للجرذان حديثة الولادة حتى عمر الفطام. استخدمت أربعة مجاميع من اناث الجرذان الحوامل نوع Wister حيث اعتبرت المجموعة الأولى سيطرة بينما تم تجريع المجاميع الثلاثة المتبقية بمحلول تنكستات الصوديوم بجرع 75، 150، و 300 ملغم/ كغم/ يوم على التوالي منذ اليوم الأول للولادة ولمدة 21 يوم. دلت النتائج على وجود ارتفاع في الزيادة الوزنية للامهات المرضعة والتي عوملت بجرعة 75 ملغم/ كغم مقارنة بالجرعتين الأخريتين اللتان أظهرتا انخفاضا في المعيار المذكور مقارنة بمجموعة السيطرة أما فيما يتعلق بالزيادة الوزنية للمواليد، فقد لوحظ أن جرعتي 75 و 300 ملغم كانتا الأفضل في تحقيق أعلى زيادة وزنية على مدى 21 يو، كذلك كان الحال في مؤشرات التطور التي شملت فترة ظهور العين وظهور الأسنان حيث أن جرعتي 75 و 300 ملغم/ كغم أظهرتا وقتا وقتا أقل في معدل ظهور الأسنان وفتحة العين مقارنة بمجموعة السيطرة، بالنسبة لوزن المبيض فقد كان الأعلى في مجموعتي 75 و300 ملغم/ كغم على التوالي بينما أظهرت الجرعة 300 ملغم/ كغم القيمة الأقل فيما يتعلق بوزن الخصية بين المجاميع قيد الدراسة. يستنتج من الدراسة الحالية أن لتنكستات الصوديوم تأثيرات ايجابية في النواحي التطورية للجرذان حديثة الولادة عند اعطاءها عن طريق الرضاعة حتى عمر الفطام.

Keywords

tungstate --- developmental --- offspring --- rat.


Article
Histological and Morphological Analysis of the Hippocampal

Author: Ahmed A.W. Hussein* MBChB, MSc ]د. احمد عبد الوهاب حسين
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 3 Pages: 323-327
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: The hippocampus is empirically assumed to have different functions, of which the best known are: the representation of self-location in cognitive dimensions, and the storage and retrieval of memory.Materials and methods: Eleven adult male albino rats were used. Brain specimens were processed into paraffin blocks, sectioned (10 µm thick), and stained using Haematoxylin and Eosin, and Luxol Fast Blue MBS counterstained with Cresyl Violet. Morphometric analysis was done through eyepiece micrometers.Results: The lamellae of the hippocampus were morphometrically evaluated in context of embryogenesis and nerve fiber content.Conclusion: This study confirmed the existence of differences in nerve fiber distribution among the subfields of the hippocampus which is attributed to differences in the embryonic development processes and to the selectivity of the different connections.Keywords: Rat Hippocampus, Lamellar Morphometry.


Article
Teratogenic effect of Fluoroquinolones in rats
التاثيرات الولاديةالتي تحدثها الكوينولات المفلورة على الجرذان

Author: ِAshwaq J. Al- Miahy اشواق جبار فليح
Journal: Thi-Qar University Journal for Agricultural Researches مجلة جامعة ذي قار للبحوث الزراعية ISSN: 22225005 Year: 2013 Volume: 2 Issue: 1 Pages: 55-69
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

The aim of the present study is to determine the effect of fluoroquinolones on the weight gain of pregnant rats , abortions and possible teratogenic effects on fetuses at the day 21 of pregnancy .Material and Methods :186 fetuses and pups of Norwegian rat were used in this study. They were obtained from 34 pregnant rats at21 day of gestation. The pregnant rats were divided to four groups . Group A received enrofloxacin in a dose of 700 mg.kg/day orally from 6 the to 12 the days of gestation . . Group B and C received ciprofloxacin and enrofloxacinin in a dose 550 and 750 mg.kg/day respectively and Group D behaved as control and received 0.5 ml/animalday of DMSO all received orally for the same period . The pregnant rats were examined for liver size ,and placenta. Results The weight gain of fluoroquinolones treated pregnant rats showed a significant decrease of( 48.35+ 2.241,44.66 + 1.629 and 45.53 + 1.810/gm)as compared to control group which was 60.83+ 2.926. Fetuses from treated mothers showed a significant . decrease in placentaland liver weight , placental weight showed a significant .decrease (p<0.05) of (0.420.0920. 39+ 0.038 and 0.38 0.052) (gm)as compared to control group ( 0.62+ 0.049). Fetuses from treated mothers showed significant decrease (p<0.05) in liver weight of (0.39 + 0.027, 0.36 + 0.026 and 0.35 + 0.027)(mg) as compared to control group which was( 0.59 0.020) Conclusion :norfloxacin, ciprofloxacin, and enrofloxacin were found to be embryotoxic by reduced placental and liver size of fetuses there fore reduced mothers weight gain.

الهدف من هذه الدراسة هو الكشف عن تأثيرات للكوينولات المفلورة (النور فلوكساسين ,والسبروفلوكساسين والانروفلوكساسين ) على مصلحة اوزان الجرذان البيضاء الحوامل, حصول الاجهاضات .ومدى حدوث التشوهات الجنينية في اليوم 21من الحمل والولادة . استخدمت 34أنثى جرذ من النوع النرويجي الأبيض وبعمر 12أسبوع بعد تزاوجها مع الذكور خصبة ومن نفس النوع وبعد التأكد من حصول الحمل (عن طريق المسحات المهبلية ) قيست اوزرنها في اليوم الأول من الحمل ثم أعطيت جرع مفرد يوميا من هذه المواد عن طريق الفم (النور فلوكساسين 700ملغم/كغم ,والسبروفلوكساسين 550ملغم /كغم ,والانروفلوكساسين 750ملغم /كغم إما مجموعة السيطرة فقد أعطيت 0.5 مل من مادة ثاني اوكسيد سلفات المثيل )من اليوم 6 الحمل ولغاية اليوم 12منه, وفي اليوم 20من الحمل استخدمت 186جنين اخذت من24أنثى فقط من تلك الجرذان لقياس أوزان المشائم والأكباد لتلك الأجنة لان باقي الإناث وبالأخص المعاملة قد تعرضت لحالات الإجهاض والحمل الكذاب ,اظهر النتائج انخفاضا معنويا (P<0.05 ) في محطة أوزان الإناث المعاملة في اليوم العشرين من الحمل والتي كانت ) 48.352.241, 44.661.629و 45.531.810) (غرام ) على التوالي عندما قورنت مجموعة السيطرة والتي كانت مصلحة أوزانها ) 60.832.926) وانخفاضا معنويا (P<0.05) في أوزان المشائم 0.39 0.038 ,0.420.092 ) و 0.052 0.38 )(غرام ) مقارنة مع مجموعة السيطرة 0.049 )  (0.62 وأيضا انخفاض معنوي (P<0.05) في أوزان أكباد الأجنة للإناث المعاملة 0.027)  0.036 +0,0.26  027. 0 (0.35 (غرام)مقارنة بمجموعة السيطرة  0.020 ) (059 غرام نستنتج من ذلك إن الكوينولات المفلورة وبالأخص النور فلوكساسين السبروفلوكساسين والانروفلوكساسين لها تأثير سلبي على أوزران أكباد الأجنة وأوزان المشائم لذلك فهي تقلل من محطة أوزان تلك الإناث .


Article
EFFECT OF PASSIVE IMMUNIZATION AGAINST INHIBIN-Α, -ΒA, AND –ΒB SUBUNITS ON SERUM GROWTH AND DIFFERENTIATION HORMONES PROFILE IN PREGNANT AND LACTATING PRIMIPAROUS WISTER RATS

Author: Jabbar A.A.Al-Sa'aidi * , Jassim M.A. AlKalby ** ,Manhal J. A. Al-Saeedi
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2015 Volume: 14 Issue: 1 Pages: 79-96
Publisher: Basrah University جامعة البصرة

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Abstract

Transforming growth factor β (TGFβ) superfamily members are closely associated with tissue remodelling events and reproductive processes. The present study aimed to determine the role of immunoneutralization of endogenous inhibin-α, βA, and βB subunits on serum growth growth and differentiation hormones profile during pregnancy, delivery, and lactation periods in primiparous female Wister rats.Eighty four pregnant primiparous female rats were assigned to 4 groups (21 per each). On 5 th and 10 th day of gestation, control was injected with saline (100μl, i.p.), Ta, Tba, and Tbb groups were injected with inhibin-α, βA, and βBantiserum (1µg in 100μl of saline, i.p.), respectively. Each group was allocated to 3 equal subgroups: pregnancy, delivery, and lactation subgroups were sacrificed on day 16 of gestation, 1st day after parturition, and 11 th day of lactation, respectively. At the end of each subgroups period, females were anesthetized, dissected and blood samples were obtained for assessment of inhibin-A, -B, activin-A , -B, -AB, GH, and prolactin levels. Serum inhibin-A concentration in Tbb group increased during pregnancy and delivery among experimental groups. In comparison between periods, Tbb group showed significantly higher level during pregnancy and decreased during delivery and lactation, whereas Ta and Tba groups recorded no difference between periods. Inhibin-B increased in control and Tba groups during pregnancy, whereas lactation period showed higher levels in Ta, Tba, and Tbb groups compared with control. Ta, Tba, and Tbb groups recorded no siginificant differences between pregnancy and delivery periods but they were significantly higher in lactation period.During pregnancy and delivery, Tbb group revealed higher levels of activin-A and lowest level of activin-B concentrations among groups, whereas activin-AB concentration increased in control and Ta groups. In comparison between periods, activin-A concentration was higher at delivery, whereas activin-B and –AB concentrations were higher at lactation. Serum GH, in Ta group, recorded higher level during the three periods among groups. In comparison between periods, the levels of GH and PRL in all groups showed higher levels at delivery followed by lactation and pregnancy.In conclusion, passive im munization against inhibin-α, βA, but not βB subunit, at 5th and 10th day of pregnancy, have ameliorating role on serum growth and differentiation hoemones profile


Article
HISTOMORPHOLOGICAL STUDY OF MESONEPHRIC KIDNEY DEVELOPMENT IN PRENATAL STAGES OF RATS (Rattus norvegicus Albinus).

Authors: Nidaa hamdi khalaf --- Shakir Mahmood Mirhish
Journal: Diyala Journal of Agricultural Sciences مجلة ديالى للعلوم الزراعية ISSN: 20739524 Year: 2016 Volume: 8 Issue: 1 Pages: 1-12
Publisher: Diyala University جامعة ديالى

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Abstract

The development of mesonephros kidney study wasdone on the rats (Rattus norvegicus Albinus), the study included detection of the timing of appearance of mesonephros kidney. The study revealed that the differentiation and development of the mesonephros kidney began in the rat at 13th day from pregnancy. The study showed that the mesonephros was poorly developed in rat,and the degeneration was began in 15th day of gestation period and end in 17 days gestation period to replace by metanephros (permanent kidney).

Keywords

mesonephros --- rat --- prenatal


Article
Histological study of the effect of Pegenumharmala seed extract on liver, liver enzymes andsome blood parameters in male Albino Rats
ﻲﻓ مﺪﻟا ﺮﯿﯾﺎﻌﻣ ﺾﻌﺑو ﮫﺗﺎﻤﯾﺰﻧاو, ﺪﺒﻜﻟا ﻰﻠﻋ ﻞﻣﺮﺤﻟا تﺎﺒﻧ روﺬﺑ ﺺﻠﺨﺘﺴﻣ ﺮﯿﺛﺄﺘﻟ ﮫﯿﺠﺴﻧ ﺔﺳارد . ﺾﯿﺒﻟا ناذﺮﺠﻟ

Author: Lina Adeeb Mahdi
Journal: journal of al-qadisiyah for pure science(quarterly) مجلة القادسية للعلوم الصرفة (فصلية). ISSN: 19972490 Year: 2015 Volume: 3 Issue: 20 Pages: 18-27
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Present study was carried out to find the effect of Pegenumharmala on liver tissue, liverfunction test and some blood parameters on experimental animals, thus for this purpose ,Fourgroups of male albino rats (Musmusculus) were subcutaneously administrated with normalsaline(0.9 % NaCl) or Peganumharmala aqueous seed extract (1%,2%,3%) mg/ kg)/bodyweight at daily interval for one month period. Thereafter, animals were scarified andspecimens from the liver were examined under light microscope for structural changes.Repeatedtreatment withPeganumharmala seeds aqueous extract caused dose- relatedstructural changes in the liver treated groups. Severe changes were observed following 2 %mg/ kg dose that were manifested by fibrosis in interstitial connective tissueand blood vesselsof the liver. repeated treatment of Peganumharmal water extract a seeds at 3% mg/ kg dosecaused severe destruction of hepatic cellnucleiand vesiculation in the cytoplasm due todegeneration in hepatic cells .In addition, dis arrangement in hepatic sinusoids and destructionin the walls of central veins wereobserved. Nuclear polymorph cellular infiltration andcirrhosis as wellas pyknotic in hepatic cell nuclei were noticed in the 1% mg/ kg dose group.Peganumharmala seedsaqueous extract at 1% and 2% mg/ kg caused slight tomoderatehistological changes in the liver manifested as degeneration and hypertrophy oftubular epithelial lining. In addition,The oral administration of extractcauses maximum fallof blood glucose level to(138 and 35.5 ) at(p<0.01) respectively with the normalratsCholesterol was decreasedsignificantly ( 0.01 ) in treatedgroup compared with control .Lowestvalue was in second dose 2% (29.0) while the highest value was in control group(148.5),significant changes (0.05) in GPT and GOT enzymes were observedbetween treatedand control group. The highest values were incontrol groups while the lowest values were intreated groupNon-significant changes were observed in the values of WBC and RBC intreatedrats compared to controls. In conclusions Peganumharmala seed extract has manyhistopathological effect on liver tissue as well as moderate effects on liver enzymes andsome blood parameters.

Keywords

Histology --- Pegnumharmala --- rat


Article
Glycogen Changes in the Rat Uterus During Different Phases of the Oestrous Cycle
دراسة التغيرات الحاصلة في الكلايكوجين لارحام الجرذان خلال الاطوار المختلفة من الدورة الودقية

Authors: Hafidh A. Al Ashou حافظ العشو --- Elias I. Shaya الياس اسحق شعيا --- Manhal S. Al Annaz منهل شاكر العناز
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2005 Volume: 16 Issue: 8E عدد خاص بعلوم الحياة Pages: 1-7
Publisher: Mosul University جامعة الموصل

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Abstract

Objective: to study the glycogen content in the rat uterus at oestrous cycle (oes).Design: an experimental study of the glycogen content in the rat uterus at (oes).Setting: done during the period of 2000-2002 in the department of anatomy Mosul Medical College.Methods: this study was carried out on 24 sexually mature virgin female rats of 13-15 weeks old and 165-270 gram weight. They were divided into four groups according to the phases in oestrus cycle 1. Metoestrus 2.dioestrus 3. Proestrus and 4. Oestrus phases, the uterine horns were divided into pieces of 3-4mm and prepared for histological examination, then sections of six micron thickness were collected and stained by alcian blue-periodic acid Schiffs’ reagent (PAS). The glycogen was shown as magenta color.Result: cellular proliferation was noticed at dioestrus and proestrus phases then followed by an increase in the glycogen content at proestrus which became highest at oestrus phase, which is mainly concentrated in the endometrium. Conclusion: this study showed increase in glycogen content at proestrus and oestrus phases mainly concentrated at the endomatrium, due to consumption of glycogen by the future nidating zygot after mating and fertilization which occur in the endometrium later

الهدف:لدراسة التغيرات الحاصلة في الكلايكوجين لارحام الجرذان خلال الاطوار المختلفة من الدورة الودقية.التصميم: دراسة تجريبية للكلايكوجين في ارحام الجرذان على فترات مختلفة من الدورة الودقية.تاريخ ومكان البحث: اجري البحث في عام 2000-2002 في قسم التشريح-كلية الطب-جامعة الموصل.الطريقة: اجريت الدراسة على 24 من اناث الجرذان البالغات جنسيا والباكرات يتراوح عمرها 13-15 اسبوعا ووزنها 165-270 غرام وقد قسمت الى 4مجاميع بحثية حسب الاطوار الودقية 1. الطور الذي يلي الودق 2.طور الاستكنان الودقي 3.الطور الذي يسبق الودق 4.طور الودق نفسه، وبعد جمع قرون الارحام وتقطيعها بحجم 3-4 ملم قطع منها شرائح نسيجية بسمك6مايكرون وصبغت بAlcian blue(PAS) وظهر الكلايكوجين بلون بنفسجي.النتائج: لوحظ زيادة الانقسامات الخلوية في طور الاستكنان الودقي والطور الذي يسبق الودق تلاها زيادة في الكلايكوجين خلال الطور الذي يسبق الودق حيث وصل ذروته في طور الودق نفسه وهذه الزيادات متركزة بصورة رئيسية في الغشاء المبطن للرحم يلي ذلك في عضل الرحم وبصورة اقل في ظهارة الرحم الخارجية.الاستنتاج: لوحظ في هذه الدراسة ان الكلايكوجين يزداد في الطور الذي يسبق الودق ويصل ذروته في طور الودق نفسه ويتركز في الغشاء المبطن للرحم لان الكلايكوجين يعتبر مادة غذائية مهمة عند انزراع البويضة المخصبة بعد الجماع والاخصاب والذي يتم بالتحديد في الغشاء المبطن للرحم.

Keywords

Glycogen --- rat --- utrus --- oestrus cycle

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