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Article
Effect of infection on semen parameters in a sample of Iraqi infertile males.

Authors: Jawad K. Al-Diwan --- Eman Al-Kaseer --- Ali A. Lazem
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2010 Volume: 52 Issue: 3 Pages: 272-274
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Several studies were carried out on association of infection with male infertility, which revealed great variations in the prevalence of genital infection in different parts of the world. This work was designed to study the effect of infection on semen parameters in a sample of Iraqi infertile males.Methods: A sample of 400 infertile male patients attending the High Institute for Embryo Research and Infertility Treatment for laboratory investigations and treatment were selected. This study was carried out during the period Nov. 2002 to April 2003, inclusive. Seminal fluid analysis was performed on samples obtained by masturbation into a glass container after 48 to 72 hours from of abstinence from sexual intercourse. Analysis (examination of volume, liquifaction, sperm count, motility percent, normal morphology percent and presence of pus cells) was performed within an hour after ejaculation. Results: Clinical infection was detected in 14% of infertile males and 29% had subclinical infection. Liquifcation time and leukocyte had significant variation between infected and non infected patients. Conclusion: Early diagnosis and treatment of genitourinary infection in necessary to reduce complication and infertility in future.

Keywords

Infertility --- semen --- infection --- Iraq


Article
Etiology of infertility in couples attending maternity hospital in Erbil
مسببات العقم عند الأزواج الذين يذهبون إلى مستشفى الولادة في أربيل

Authors: Khanzad Hadi Rashid --- Aza Bahadeen Taha
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2013 Volume: 17 Issue: 1 Pages: 322-330
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: Infertility remains a global health challenge with devastating psycho-social consequences in many communities and an underlying long-term risk for separation of the couple, also remains a major clinical and social problem. Infertility is defined as inability of a couple to conceive naturally after one year of intercourse. An understanding of the medical causes of infertility is crucial in order to reduce incidences of infertility and for improving the clinical management of infertility. The aim of the study was to determine the causes of infertility in couples. Methods: A retrospective study was done in IVF Unit at Maternity Teaching Hospital in Erbil. Data of infertile couples were collected from already prepared patient’s files. Results: The frequency of primary and secondary infertility among women was 62.97% and 37.03%, respectively. Among 370 couples, 35.68% had female factor, 29.46% had both male and female factors, and 19.73% had male factor, while 15.14% of the couples had unexplained infertility. Conclusion: Primary infertility was common health problem in couples, also the causes of infertility in women is noticed more than men.

Keywords

Infertility --- Etiology --- Hormones --- Semen


Article
Correlation of serum and seminal plasma human growth hormone concentration with semen parameters of normozoospermic and infertile men
علاقة تركيز هرمون النمو البشري في المصل والبلازما المنوية مع معايير المني للرجال سوي النطف والمصابين بالعقم

Author: محمد باقر محمد رشاد
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: 22180265 Year: 2011 Volume: 1 Issue: 2 Pages: 18-24
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundBackground: Human growth hormone (HGH) is undisputedly a potent hormone with a wide variety of biological effects. The anabolic actions of human growth hormone have made it attractive as a potential agent forcatabdicproblems in a wide range of clinical conditions,QbjectiveI n the present study, we investigated the possible relationship between levels of H G H in serum (S. HGH) and seminal plasma (Sp. HGH) and semen parameters in normozoospermic (NZS) men and infertile patients.materials and methods:In total, 66 infertile patients with azoospermia (AZS; no. 52), asthenozoospermia [AsZS; no, 6) and oligoasthenoteratozoosperrriia (OATZS; no. 8), and normozoospermicmen (NZS; no. 12)were included in this study.. From each subject semen and blood samples were taken to assess seminal plasma and sperm para meters and levels of S. HG H and Sp. HG H; respectively.resultsThe highest 5. HGH was evaluated in AZS patients when compared to NZS and other infertile groups. Significant elevation {P<0.05) in the level of S. HGH was assessed for AZS patents as compared to NZS men. in contrast the level of Sp. HGH in NZS men was significantly elevated (P<0 Q5)when compared to AZS patients only. Positive and significant correlations were noticed between levels of Sp. HGH and semen liquefaction time in NZS, AZS and OATZS groups, in NZS men, positive and significant correlation were observed between Sp. HGH and semen volume and negative and significant correlations were detected between Sp. HGH and percentages of sperm motility, progressive motility, immobility and agglutination.conclusionOur study represents the first study in Iraq showing that the level of HGH in serum and seminal plasma affect male fertility and sperm parameters in normozoaspermic males and infertile patients. Further biochemical and molecular studies are recommended to investigate the mode of action of HGH and its receptors in relation to fertility.


Article
EFFECT OF VITAMIN C INJECTION ON SEMEN QUALITY OF AWASSI RAMS.

Author: H.K.J.Al-saab*
Journal: Diyala Journal of Agricultural Sciences مجلة ديالى للعلوم الزراعية ISSN: 20739524 Year: 2015 Volume: 7 Issue: 2 Pages: 12-19
Publisher: Diyala University جامعة ديالى

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Abstract

This study was conducted in the Animal Farm, College of Agriculture, University of Baghdad, from march to may 2013, to clarify the effect of subcutaneous vitamin C injection on semen characteristics of Awassi rams. Six rams (1.5-2 years old) were randomly divided into three groups of 2 rams. Rams in T1and T2 were subcutaneously injected, every other day with dose of 50 and100 mg/kg body weight respectively ,for 45 days while control group in T0 received normal saline. The results showed a significant increase (P<0.05) in the The sperm concentration of T1 as compared with control group, while there were no significant differences among treated groups(T1 and T2). Group 2 showed a significant (P<0.05) increases in mass activity, individual motility and live sperm percentage. There was a significant decrease in individual motility and percentage of live sperm in T2 as compared to T1 and control group.In conclusion, despite of the significant improvement of some semen trail as in T2 group, the lower dose of vit. C(less than 50 mg/kg of body weight) was recommended represented the skin tearing(T2group) as well as the redness and sensitive skin in T1 group.

Keywords

words:Vitamin C --- Semen --- Rams


Article
Effect of adding Cinnamomum camphora on the testosterone hormone and reproductive traits of the Awassi rams

Author: Tamara N. Dawood
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 36-45
Publisher: University of Kufa جامعة الكوفة

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Abstract

This study was aimed to find out the effect of camphor on sexual behavior in male rams. Eight sexually mature Awassi rams were randomly divided into two groups, the experimental group was received Cinnamomum camphora (20 mg/kg/ day) orally with their concentrated diet for four weeks; while the other group (control) were feed on the same diet without adding camphor. Semen was collected from the rams and the results showed that camphor reduced the semen volume, mass activity, testes length and live sperm percentage while there were insignificant differences in the testes circumference, semen concentration, individual motility and testosterone hormone level between the treated and control groups. In addition it was noticed that the abnormal percentage of sperm was significantly higher in camphor group compared with the control group.

Keywords

testosterone --- Awassi rams --- Semen --- camphor


Article
Effects of Metformin on Sperm Parameters in Mice: as a model for human being

Authors: Muhammad –Baqir M-R Fakhrildin --- Mohammad 0. Selman --- Ahmed. Kh. Rashid
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: 22180265 Year: 2016 Volume: 6 Issue: 1 Pages: 45-60
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Metformin is anti-hyperglycemic effect due to decrease of hepatic glucose production. Another effect, leading decrease insulin resistance, regarded a safe drug, treatment of choice for overweight.Objective:To investigate the effects of 3 concentration of MF administered orally to male mice on sperm parameters as model for human being.Materials and Methods:In current study, 80 male mice were taken with age ranging between 1.5-2 month and weights ranging between 25-30 grams males classified into four groups of a control group (G1) and treated group (G2, G3 and G4) doses of MF were employed, 0.2, 0.4, 0.6 mg/mice daily to groups, given orally for 6 weeks and then the animals were scarified.ResultsResults have revealed a significant difference (P < 0.05) in sperm concentration for 3 groups compared to G1 group. Progressive motility (%) appeared with significant difference (P< 0.05) for G3 group as compared to other treated and G1 group. Furthermore, the result illustrated a significant decreased (P< 0.05) in the abnormal sperm morphology for G3 group compared with treated and control. However, sperm agglutination (%) confirmed that the G3 group exhibited highly statistical significant decrease (p≤0.001) compared to G1, G2 and G4.ConclusionsFrom results of the present study, it was concluded that the administration of MF doses reduce sperm concentration .while, 0.4mg dose improve certain sperm parameters.


Article
Cryopreservation of goat semen using Soy milk as alternative of egg yolk
تجميد السائل المنوي للماعز باستخدام حليب الصويا كبديل لصفار البيض

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Abstract

This study was carried out at Research Station, and laboratory, Animal Production Dept., Agric. College, Al-Muthanna Univ., during /2016 to 2017 to improve the quality of cryopreservation semen goats by identifying the best concentration of soy milk and comparing it to Egg yolk, besides glycerol 7% was used as a check treatment. The results showed a significant superiority( P≤0.05) for the TFCSM (Consists of tristtmen 3.63 g/100 ml, fructose 0.5 g/100 ml and citric acid 1.99 g/100 ml+5% soy milk) and difference in cry protectants results. G7% with TFCSM showed significant superiority( P≤0.05) in the percentage of progressive motility Type A, the non-progressive motility type D, Agglutination, live sperm with averages 18.88, 45.44, 3.86 and 76.50, respectively. The EG5% with TFCSM showed significant superiority( P≤0.05) in the percentage of progressive motility type A and B, the non-progressive motility type C and D and the percentage of normal sperm, with averages 17.76, 20.15, 15.82, 45.64 and 88.51, respectively.

اجريت هذه الدراسة في محطة الابحاث الثانية التابعة لكلية الزراعة/جامعة المثنى ومختبر الدراسات العليا التابع لقسم الانتاج الحيواني /كلية الزراعة/جامعة المثنى للمدة من2102016 ولغاية 9/7/2017 بهدف تحسين نوعية السائل المنوي المجمد للماعز الافغاني, لمقارنة مخفف حليب الصويا بمخفف صفار البيض التقليدي, للتأكد من إمكانية استبدال الجليسيرول بموانع تجميد ذات وزن جزيئي منخفض مثل الاثلين كلاي كول إذ استخدم الجليسيرول كمعاملة مقارنة بتركيز 7% واستخدم ثلاث تراكيز مختلفة من الاثلين كلاي كول (3, 5 و7% على التوالي) ومقارنة تداخل كل واحد من موانع التجميد على مخفف حليب الصويا وصفار البيض. وجد تفوق معنوي P≤0.05)) لمخفف الTFCSM (تكون من الTris 3.63غم/100مل, fructose 0.5غم/100 مل وcitric acid 1.99 غم/100مل+5% حليب الصويا) و بتباين نتائج موانع التجميد اذ اظهر ال%G7 مع الTFCSM تفوق معنويP≤0.05)) في النسبة المئوية للحركة التقدمية من النوع A, الحركة غير التقدمية من النوع D, تكتل النطف, النطف الحية اذ كانت المتوسطات 18.88, 45.44, 3.86 و 76.50 على التوالي. أما الEG5% مع الTFCSM فاظهر تفوقآ معنويآ P≤0.05)) في النسبة المئوية للحركة التقدمية من النوع A و B والحركة غير التقدمية من النوع C وD والنسبة المئوية للنطف الطبيعية اذ كانت المتوسطات 17.76, 20.15, 15.82, 45.64 و 88.51 على التوالي.


Article
The effect of treatment by L-carnitine for infertile men on semen parameters
تأثير الكارنتين في علاج الرجال العقيمين

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Abstract

L-Carnitine (LC) is highly concentrated in the epididymis and plays a crucial role in sperm metabolism and maturation.They are related to sperm motility and have antioxidant properties. The objective of this review is to summarize the multiple roles played by LC in male reproduction, and to highlight their limitations as well as their benefits in the treatment of male infertility. A variety of studies support the conclusion that LC at total daily amounts of at least 500mg per day can significantly improve both sperm concentration and total sperm counts among men with astheno – or oligoasthenozoospermia. Although many clinical trials have demonstrated the beneficial effects of LC in selected cases of male infertility. Additional, a well – designed study is necessary to further validate the use of carnitines in the treatment of patients with male infertility, specifically in men with poor semen quality .

(الكارنتين) يتركز بشدة في البربخ ويلعب دورا حاسما في استقلاب الحيوانات المنوية والنضج.ترتبط بحركة الحيوانات المنوية ولها خصائص مضادة للأكسدة. الهدف من هذه المراجعة هو تلخيص الأدوار المتعددة التي تلعبها الكارنتين في إعادة إنتاج الذكور، وإبراز دورها وكذلك فوائدها في علاج العقم عند الذكور. تدعم مجموعة متنوعة من الدراسات الاستنتاج القائل بأن الكارنتين الكميات اليومية التي تستخدمها الشخص والتي لا تقل عن 500 ملغ في اليوم يمكن أن يحسن بشكل كبير كل من تركيز الحيوانات المنوية وعدد الحيوانات المنوية الكلي لدى الرجال المصابين بالوهن أو قلة في عدد الحيامن. على الرغم من أن العديد من التجارب السريرية قد أثبتت الآثار المفيدة لي الكارنتين في حالات معينه ومختارة من العقم عند الذكور، إضافية، دراسة مصممة بشكل جيد ضروري لمزيد من التحقق من صحة استخدام الكارنتين في علاج المرضى الذين يعانون من العقم عند الذكور، وخاصة في الرجال الذين يعانون من نوعية السائل المنوي الفقيرة ( قليلة في العدد والنشاط).


Article
EFFECT OF AQUEOUS EXTRACT OF TURMERIC ON PATHOGENIC BACTERIA ISOLATED FROM SEMEN IN A SAMPLE OF IRAQI INFERTILE MEN

Author: Rehab Sh Al-Maliki رحاب شفيق المالكي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2012 Volume: 10 Issue: 2 Pages: 105-110
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background: Contamination of semen with sexually and none sexually transmitted bacteria plays an important role in male infertility, contaminating bacteria should be eradicated by antibiotics, but most of bacteria become resistant to available antibiotics. Therefore most researchers search to find other antibacterial agents.Objective: To evaluate the frequency of bacteria in semen of infertile men and investigate the antibacterial activity of aqueous extract of turmeric (AET) on certain isolated bacteria from semen of infertile men compared with that of doxycycline as a standard antibiotic.Methods: Forty two semen samples obtained from infertile men attending The Higher Institute of Infertility Treatment and Assisted Reproductive Technology were evaluated bacteriologically using standard bacterial culture. Then investigated the antibacterial activity of AET on certain isolated bacteria from semen of infertile men compared with doxycycline using disc diffusion method.Results: Out of 42 semen samples of infertile men, 35(83.03%) were infected with different bacterial species (spp.) accompained with highest incidence rate. The overall frequency of N.gonorrhea and S.epidermides was 8(19%) and for remaining bacterial isolated from semen (S.aureus, G.vaginalis and E.coli) were 6(14.28%). Comparable antibacterial activity of ATE and doxycycline was found to be evident against most isolated bacteria (P<0.001) in mean inhibition zones of AET between Gram’s positive and Gram’s negative bacteria (23.35±0.68 and 8.35±1.52, respectively).Conclusion: Most of isolated bacteria from semen of infertile males had high frequency, which were generally accepted as pathogenic bacteria. Antibacterial activity of AET was comparable with doxycycline against most isolated bacteria and it was found more effective on Gram’s positive than Gram’s negative bacteria.Keywords: Turmeric, pathogenic bacteria, semen, infertile men


Article
Bacterial Contamination of Imported Bulls Frozen Semen
التلوث البكتيري في قصبات السائل المنوي المستورد للثيران

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Abstract

One hundred frozen semen straws (0.5 ml), have been taken from imported bulls, the frozen semen examination as well as evaluation in addition to bacteriological evaluation whether counting, isolation and antibiotic sensitivity, the results in current study showed all bacterial isolates were identified by using different biochemical tests and API–20 E system that used later to confirm identification, all isolated bacteria found in imported frozen semen were gram negative.45 bacterial isolates in imported frozen semen distributed in 42 isolates Stenotrophomonas maltophilia and 3 isolates Pseudomonas aeroginosa, antibiotic susceptibility test for isolated bacteria by using 14 single antibiotic discks of commonly used drugs, most isolated Steno. maltophilia was susceptible to Ceftazidime, Ciprofloxacin, Cefotaxime, Gentamicin, Amikacin, Vancomycin, Erythromycin, Chloramphenicol and Tetracyclin. The results of current study showed individual movement 61.3±2.00, viability of sperm 76.2±2.06, percentage of dead sperms 23.8±2.06, and abnormal sperms 13.54±0.67.

أخذت 100 قصبه من السائل المنوي المجمد المستورد 5. 0مل, أجريت فحوصات تقييم السائل المنوي واختبارات العد والعزل الجرثومي على العينات واختبار فحص الحساسية للمضادات الحيوية المختلفة. كل الجراثيم التي عزلت شخصت, وأجريت الفحوصات الكيموحيوية التقليدية والتشخيص بنظام API-20E لتشخيص الأنواع الجرثومية المعزولة. كانت جميع الجراثيم المعزولة من عينات السائل المنوي المجمد المستورد سالبه لصبغة كرام, خمسة وأربعون عزلة جرثومية عزلت من السائل المنوي المستورد موزعه في 42 عزله منStenotrophomonas maltophilia و3 عزلات من Pseudomonas aeruginosa. اختبرت الحساسية للجراثيم المعزولة وتم استخدام 14 نوع من الأقراص المفردة للمضادات الحيوية الأكثر استخداما, أغلب عزلات Steno. maltophilia كانت حساسة للـ Ceftazidime, Ciprofloxacin, Cefotaxime, Gentamicin, Amikacin, Vancomycin, Erythromycin, Chloramphenicol وTetracyclin, من ناحية أخرى كانت جميع الجراثيم المعزولة مقاومه بنسبة 100% لكل من الـ penicillin StreptomycinوAmoxicillin. بينت نتائج الدراسة الحالية للعينات المستوردة ان الحركة الفردية 61.3±2.00 وحيوية الحيامن 76.2±2.06 والنسبة المئوية للحيامن الميتة 23.8±2.06 أما النسبة المئوية للحيامن المشوهة فكانت13.54±0.67.

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