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Article
DNA content of Spermatozoa with respect to Seminal Sperm Concentration using a Microchemical Spectrophotometric Method

Authors: Faraid A. AL- chalabi --- Munaf S. Daoud*
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2006 Volume: 48 Issue: 4 Pages: 410-412
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: To determine the DNA content in subfertile patients and to correlate it
with seminal sperm concentration.
Design: Prospective observational study.
Setting: College of Medicine, Dept. of Physiological chemistry and Institute for
embryo Research and infertility treatment-University of Baghdad.The study was conducted through years 2004-2005.
Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (µg/ml) was estimated using a microchemical spectrophotometric method.
Results: The three groups were statistically of significant difference( P <0.01 ).The D N A concentration per spermatozoan in Gr.III ( 1 20 million/ml) was higher than the other two groups It was also noticed that there was a statistically significant (P< 0.05 ) correlation in Gr.III b etween D N A content and sperm concentration . No significant (P >0.05 )correlation was observed in the other two groups (Gr. I & Gr. II ).
Conclusion: Significant positive correlation was obtained between sperm concentration and DNA content. in the oligozoospermic subfertile patient , but no significant correlation was found in the normozoopermic patients . D N A content per spermatozoan from oligozoospermic patients was higher than that in normozoospermic patients.


Article
Effect of stress on semen quality in a population of infertile human

Author: Dr. Edress M. Ameen
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2009 Volume: 12 Issue: 1 Pages: 167-174
Publisher: University of Kufa جامعة الكوفة

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Abstract

The aim of the present study was to assess the relationships between the stress which results from the work and human semen quality. The work stress which was studied includes two types; quality stress (easy and moderate to hard performing work/day) and quantity stress (number of hours performing work/day. Results are elaborated by using independent t-test statistical method. From fifty two men which has an easy and moderate work and forty eight men with daily hard job, we determined significant negative (p<0.05) influence of stress on sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability. Quantity stress showed that a men with 7-10 hrs performing job/day has a significant (p<0.05) lower; sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability compare with 4-6 hrs performing job/daily. No significant differences were recorded between quality and quantity stress in which means that both types of stress has the same effect on semen quality.

إن هدف الدراسة الحالية هو تقييم العلاقة بين الجهد الناتج من العمل و نوعية السائل المنوي في الإنسان. تم دراسة نوعين من جهد العمل هي ، الجهد النوعي (أداء عمل سهل و معتدل إلى شاق/ يوم) والجهد الكمي (عدد ساعات العمل المنجزة / يوم). تم إجراء التحليل الإحصائي باستخدام اختبار t . تم الحصول على تأثيرات سلبية معنوية (p<0.05) للجهد على تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و كذلك النسبة المئوية للنطف الحية عند استخدام اثنتان و خمسون رجلا ينجزون عملا سهلا إلى معتدل و ثمانية و أربعون رجلا يؤدون عملا شاقا. أظهرت نتائج الجهد الكمي بان الرجال الذين يعملون لمدة 7-10 ساعات في اليوم لديهم نسبة اقل معنويا (p<0.05) من تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و النسبة المئوية للنطف الحية مقارنة مع الذين يعملون لمدة 4-6 ساعات في اليوم. لم تسجل أية فروقات معنوية مابين الجهد النوعي والكمي و هذا يدل على ان لكلا النوعين من الجهد نفس التأثيرات على نوعية السائل المنوي.


Article
Changes in seminal antioxidant defenses in Iraqi patients with unilateral and bilateral varicocele

Author: Issam Salman Al-Azzawi
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2014 Volume: 13 Issue: 1 Pages: 1-7
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background : The exact pathogenic mechanism by which varicoceles cause impaired testicular function remains poorly understood . One of the possible mechanisms is the generation of seminal oxidative stress. In addition, the impact of bilateral varicocele on spermatogenesis is still poorly studied . Aims : To study the changes in seminal antioxidant defenses in relation to unilateral and bilateral varicocele, and to correlate them with changes in sperm concentration and total motility. Patients and methods : A prospective, randomized, controlled study , whereby 60 semen samples were collected and analyzed. Twenty samples were from healthy subjects , having no varicocele ( control group ), 20 samples from patients with unilateral varicocele and 20 samples from patients with bilateral varicocele . For each semen sample, measurement of the seminal concentration of the antioxidants ( Albumin, Glutathione peroxidase and Superoxide dismutase ) was done and the seminal concentration of the oxidative stress marker, Malondialdehyde. In addition sperm concentration and total motility were also estimated. Comparison of the results in the 3 groups was done . Results : Measurement of semen parameters revealed a drop in the mean value of the 3 antioxidants and an increase in the mean value of Malondialdehyde, which was statistically significant in the 2 varicocele groups in comparison with control, and in bilateral varicocele in comparison with unilateral varicocele . Another drop was in the mean sperm concentration and mean percentage of sperm total motility, which was statistically significant in the 2 varicocele groups in comparison with control, but was statistically not significant between the 2 varicocele groups . Conclusions : Bilateral varicocele causes more significant drop in seminal antioxidant defenses than unilateral varicocele which seems to add a more negative impact on spermatogenesis .


Article
Sperm DNA content: An overall correlation study with sperm count, motility and morphology

Author: Munaf S. Daoud*Ph.D د. مناف صالح داود
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2007 Volume: 49 Issue: 2 Pages: 277-280
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: To determine the DNA content in subfertile patients and correlate it with seminal sperm parameters, (count, motility and morphology).Design: Prospective observational study.Setting: College of Medicine, Department of physiological chemistry and Institute for Embryo research and Infertility treatment, University of Baghdad.Methods: A random sample of 58 subfertile male patients undergoing semen evaluation and their age ranged from 20-45 years were studied. Semen samples were assessed for seminal sperms (count , motility and morphology) by direct light microscopy. Sperm DNA content was estimated using a microchemical spectrophotometric method .The data were categorized into four groups according to normal and abnormal sperms count ( million / ml ),percent motility and percent morphology. The normal limits of those sperm function parameters were according to WHO criteria. The four groups were named as Normozoospermic (NZS) (control) , Normoasthenozoospermic (NAZS) ,Normoasthenoteratozoospermic (NATZS) and oligoasthenoteratozoospermic (OATZS) groups.Results: The Mean ± SD of the four groups were of significant difference (P> 0.05 ) with respect to sperm count, sperm percent motility and morphology with exceptions of certain groups. Moreover, a non significant difference (P< 0.05) was found with respect to DNA content (µg /ml) and (µg/sperm) except for Gr. IV which showed a significant difference when compared to others.The correlation coefficients (r values) between sperm count and sperms motility, morphology and DNA content (µg/ml) were non significant in the four groups. Noticeably, the DNA content (µg/sperm) was statistically of significant (P>0.05) negative correlation with sperm count in all groups.Conclusion: All groups were of significant difference (P<0.05) among their Mean ±SD values of their count, motility and morphology. There were few exceptions. No significant differences (P>0.05) were found with respect to DNA content (µg/ml or µg/sperm) except for oligoasthenoteratozoospermic group compared to others. No significant correlation was found between sperm count and each of sperms motility , morphology and DNA content (µg/ml) in the four groups .However, the DNA content (µg/sperm) had a statistically significant (P<0.05) negative correlation with sperm count in all groups.Keywords:DNA, sperm concentration(count million/ml ), Normozoospermic(NZS),Normoasthenoszoopermic(NAZS),Normoasthenoteratozoospermic(NATZS), Oligoasthenoteratozoospermic (OATZS ) .


Article
The study of Oxidative stress, Sperm chromatin abnormality and seminal parameters in different groups for infertile patients
دراسة الجهد التاكسدي وكروماتين النطف غير السوي والمعالم المنوية لمجاميع مختلفة من مرضى العقم

Authors: Mohsin K. AL-Murshdi محسن كاظم المرشدي --- Yehya K. AL-Sultani يحيى السلطاني
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2016 Volume: 8 Issue: 3 Pages: 425-433
Publisher: University of Kufa جامعة الكوفة

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Abstract

The study was carried out between January 2014 and October 2014 including one hundred and fifty five infertile men who attended the Fertility center in AL-Sadr Medical City. The Objective of this study was to study of oxidative stress, sperm chromatin abnormality and semen quality including ( semen volume, Liquefaction time, sperm concentration, motility, and normal morphology. The results of the study showed that there was a significant increase (p<0.05) in the semen volume, sperm concentration, progressive motility and normal morphology for unexplained compared to five abnormal semen groups. Also it was a significantly decrease (p<0.05) in liquefaction time, leukocyte concentration, MDA concentration and sperm chromatin abnormality for unexplained compared with abnormal semen groups. This study supports the conclusion that oxidative stress in seminal plasma is the main causes of sperm chromatin defect with defect of semen parameters and related with male infertility.

اجريت هذه الدراسة بين شهر كانون الثاني لسنة 2014 وتشرين الاول لسنة 2014 وشملت مائة وخمسة وخمسون من الرجال العقيمين الذين راجعو مركز العقم في مدينة الصدر الطبية . هدفت الدراسة الى معرفة العلاقة بين الجهد التاكسدي وكروماتين النطف الغير السوي ومعالم المني المتضمنة ( حجم المني و زمن الاماعة وتركيز النطف والحركة التقدمية للنطف والشكل السوي للنطف). اظهرت نتائج هذه الدراسة زيادة معنوية (p<0.05) في حجم المني وتركيز النطف والحركة التقدمية للنطف والشكل السوي للنطف لمجموعة العقم غير المفسر مقارنة بخمسة مجاميع اخرى من العقم ، كذلك اظهرت نتائج الدراسة انخفاضا معنويا ( p<0.05) في زمن الاماعة وتركيز كريات الدم البيض وتركيز الـ MDA ونضوجية كروماتين النطف غير السوي لمرضى العقم غير المفسر مقارنة بالمجاميع الاخرى من العقم . استنتجت هذه الدراسة بان الجهد التاكسدي هو سبب رئيسي لضرر كروماتين النطف وكذلك ضرر معالم المني مما يؤدي الى حصول العقم .


Article
Effect of age in semen quality for normozoospermic men
تأثير العمر في نوعية المني لدى الأشخاص سوي النطفNORMOZOOSPERMIA

Author: muhsen kadeum محسن كاظم حسن
Journal: Al-Kufa University Journal for Biology مجلة جامعة الكوفة لعلوم الحياة ISSN: 20738854 23116544 Year: 2012 Volume: 4 Issue: 2 Pages: 91-98
Publisher: University of Kufa جامعة الكوفة

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Abstract

Forty semen samples of normozoospermic men were examined samples divided according to age from 20- 50 year and above . The result showed significant decrease (P<0.01) of semen volume, sperm concentration, sperm motility percent and sperm grade activity, while the result showed significant increase (P<0.01) of liquefaction time too no significant effect in normal sperm morphology percent. In conclusion, the age has important role of effect on sperm and semen parameters that may be related with men ability for fertilization.

شملت هذه الدراسة فحص (40) عينة من السائل المنوي للأشخاص سوي النطف (normozoospermia men) حيث قسمت العينات حسب الأعمار من 20 سنة الى 50 سنة فأكثر.هدفت الدراسة لمعرفة تأثير العمر في نوعية السائل المنوي , أظهرت النتائج أنخفاض معنوي (p<0.01) في حجم السائل المنوي وتركيز النطف والنسبة المئوية للنطف المتحركة ودرجة نشاط النطف , في حين لوحظ زيادة معنوية (p<0.01) في زمن الأماعة ولم يلاحظ أي تأثيرمعنوي للعمر في النسبة المئوية لشكل النطف السوية.تبين من نتائج الدراسة أن تقدم العمر يؤثر سلبا وبدلالة معنوية ( (P<0.01في كل من حجم المني وتركيز النطف والنسبة المئوية للنطف المتحركة ودرجة نشاط النطف, في حين لوحظ بأن تقدم العمر يؤثر في زمن الأماعة وبزيادة معنوية (p<0.01) ومن نتائج هذا البحث يظهر أن العمر يلعب دورا كبيرا في التأثير في نوعية المني وبالتالي قد يؤثر في قدرة الرجل على الأخصاب.

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