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Evaluation of the Viability of Sperms with DNA Fragmentation in Infertile Men

Author: Ghassan Thabit Saaid Al-Ani
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2009 Volume: 8 Issue: 2 Pages: 172-179
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:The sperm DNA integrity is a vital factor to transmit the genetic material and complete the process of the formation of new generation. Many studies tried to explain the male infertility of unknown cause as a result of the presence of high percentage of sperms with DNA damage and found its correlation with the standard seminal analysis parameters.OBJECTIVE:This study was conducted to find the correlation between percentage of sperm death and percentage of sperms with DNA fragmentation and to calculate the percentage of live sperms with DNA damage.METHODS:Thirty four infertile patients were included in this study. And twenty fertile patients were volunteered as controls. Standard clinical semen analysis was performed according to World Health Organization (1999) criteria. The stain eosin–Y was used to assess sperm viability. The Terminal deoxynucleotidyl transferase-mediated dUTP- nick end labeling assay (TUNEL) was performed to observe the sperms with DNA fragmentation.RESULT:The percentage of sperms with DNA damage in the seminal sample was significantly positively correlated with the percentage of dead sperms (r=0.888; p=0.0001) and the percentage of sperms with abnormal morphology (r=0.707; p=0.0001). The percentage of live sperms with DNA damage was about 7% in infertile samples and about 11% in healthy samples.CONCLUSION:The small percentage of live sperms with DNA fragmentation in ejaculate could not explain idiopathic male infertility. This study concluded also that sperm DNA fragmentation could be considered as an independent parameter of sperm quality to differentiate infertile from fertile semen samples


Article
Analysis of DNA Damage and Oxidative Stress in Human Spermatozoa and Some Biochemical Changes in Seminal Plasma and their Correlation with Semen Quality of Infertile Men

Authors: Ghassan T. Alani --- Sermed S. Khonda --- **,Hedef D. El Yaseen
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 1 Pages: 81-88
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Numerous studies have shown the presence of DNA strand breaks in human ejaculated spermatozoa. The nature of this nuclear anomaly and its relationship to patient etiology is however poorly understood.OBJECTIVE:This study was done to investigate the relationship between nuclear DNA damage, assessed using the TUNEL assay and a number of biochemical parameters including zinc level, copper level, and the malondialdehyde (MDA) level as an indicator of the lipid peroxidation and also the standard general seminal fluid examination.MATERIAL AND METHODS:In this study 100 seminal samples were tested. Seventy five infertile patients aged (30.33±5.96) and 25 normal people aged (29.84±6.434) were included as a control. All tests were done to all the samples except the DNA fragmentation which was done to 60 samples only (45 patients and 15 normal).RESULTS:A positive relationship between DNA damage level and infertility was found. Also there were higher levels of MDA level and DNA damage in spermatozoa of infertile patients (P=0.001). We found also absence of difference in the copper level between the two groups. Regarding seminal zinc level we found that there was a low zinc level in infertile patients (P=0.001). Non-significant correlations between zinc level and the percentage of DNA fragmentation and the MDA levels were obtained.CONCLUSION:This study suggested that sperm DNA analysis might be better discriminator between infertile and fertile men than standard semen analysis also oxidative stress and sperm DNA damage contributed to sperms dysfunction. The infertile samples associated with high levels of oxidative stress and low seminal zinc levels.

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