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Article
13- STUDY THE EFFECT OF DIFFERENT CULTURE MEDIA ON SOME FROZEN SEMEN CHARACTERISTICS OF IRAQI BLACK LOCAL GOAT SPERMATOZOA

Author: Khairi G. Al-Rikabi ,Abdulrazak N. Khudair ,Taher A.Fahad
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2018 Volume: 17 Issue: 1 Pages: 147-154
Publisher: Basrah University جامعة البصرة

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Abstract

The semen was collected from five adult Iraqi black local bucks by theartificial vagina and using estrous induced doe the semen was diluted with egg yolk10% extender and frozen by liquid nitrogen for three months, macroscopic andmicroscopic evaluation were done after collection, dilution and thawing, threemedia(TCM-199, DMEM and SOF)were used to see the their effect on activation ofspermatozoa. The results showed that TCM-199 media presented higher significancein sperm motility, progressive motility compared with DMEM and SOF media.There was a great decrease in the concentration of spermatozoa after activation usingthese media which didn’t differ significantly among them and they were highersignificantly compared with control in normal morphology.

Keywords

Bucks --- DMEM --- Sperm motility


Article
Leukocytospermia and sperm quality, in seminal fluid samplesof infertile men

Author: Mohammed AL-Janabi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 1 Pages: 25-27
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

BackgroundWhite blood cell are present in most human ejaculates, but leukocytospermia may reflect an underlying pathological condition. This study evaluated the relationship between leukocytospermia and standard semen parameters.ObjectivesTo investigate the presence of correlation between the leukocytospermia , sperm motility and sperm concentration in infertile Iraqi men.MethodsA total of 154 semen analyses of infertile men were included inthis study.All semen analysis were performed within 1 hour, Data were collected for sperm motility type A and B, concentration of both leukocyte and spermatozoa. The mean numbers of motile sperms and the leukocytes were obtained for each specimen. 10 micro liter of seminal fluid were transferred on a slid and covered by a cover slip, then the mean number of motile sperm, leukocyte and concentration were obtained by reading 25 MicroscopicfieldsResul1.;;The results indicated a significant relationship between the reduction in the number ofleukocytes and the increase in the number of motile spermatozoa and sperm concentration r = -0.731 and r = - 0.77 respectively. But the mean number of motile sperms was weakly correlated with the sperm concentration r = 0.58. Conclusion :Both the mean number of motile sperms and sperm concentration were significantly correlated with the increasing number of leukocytes . That refers to improvement in motile sperm and sperm concentration with the reduction of leukocytes. Keyword: Seminalfluid, Leukocytospermia , Sperm motility.


Article
EFFECT OF HONEY ON SPERM CHARACTERISTICS AND PREGNANCY RATE IN MICE
تأثير العسل في خصائص النطف ومعدل الحمل لدى الفئران

Author: Ilaf Hassan Hadi إيلاف حسن هادي
Journal: Bulletin of the Iraq Natural History Museum مجلة متحف التاريخ الطبيعي العراقي ISSN: Print ISSN: 10178678, Online ISSN: 23119799 Year: 2017 Volume: 14 Issue: 3 Pages: 223-233
Publisher: Baghdad University جامعة بغداد

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Abstract

The aim of the current study is to demonstrate the effect of honey on the sperms characteristics (sperm concentration, sperm motility, grade of activity and sperm normal morphology) as well as pregnancy rate in mice. Sperms were obtained from caudal epididymis of male mice and prepared by adding10% of honey to the IVF medium using direct sperms activation technique for 30 minute incubation period before artificial insemination. The study revealed a significant (P > 0.05) increase in active sperm motility (grade A and grade B) 49% and pregnancy rate 53.3% in female mice artificially inseminated with sperms. On the other hand, there were no significant differences in sperm concentrations and normal sperm morphology. In conclusion, the honey was beneficial in improving male fertility of mice by enhancing sperm motility and pregnancy rate of female mice.

أجريت الدراسة بهدف بيان تأثير إضافة العسل بتركيز 10% الى الوسط الزرعي الخاص بتنشيط النطف على تركيز النطف ونشاطها والمظهر الخارجي لها وعلى معدل الحمل في اناث الفئران المحقونة بالنطف المنشطة بالطريقة المباشرة، وقد استخدمت الفئران كموديل تجريبي للبائن. تم الحصول على النطف من ذيل البربخ لذكور الفئران واستخدم العسل بتركيز 10% والممزوج مع الوسط الزرعيIVF عند تنشيط النطف في الزجاج لمدة نصف ساعة ثم حقن النطف بطريقة التلقيح الاصطناعي. أظهرت النتائج ان اضافة العسل الى الوسط الزرعي قد أثر تأثيراً إيجابياً على حركة النطف التقدمية 49%)) مقارنة مع مجموعة السيطرة. فضلاً عن وجود زيادة معنوية 0.05)>(P في معدل الحمل(53.3%) عند أناث الفئران الملقحة بالنطف المنشطة بإضافة العسل الى الوسط الزرعي. فيما اظهرت الدراسة عدم وجود زيادة معنوية في تركيز النطف والمظهر الخارجي لها. أشارت الدراسة أن مكونات العسل المختلفة وخاصة الكاربوهيدرات والمعادن و الفيتامينات ومضادات الاكسدة .قد أثرت إيجابياً على نتائج مختلف معايير الدراسة لذا يمكن الاستنتاج بأن اضافة العسل للوسط الزرعي الخاص بالنطف قد عزز حركة النطف ونشاطها ومعدل الحمل في الفئران. يمكن الاستفادة من هذه النتائج عند الإعداد لبرامج الاخصاب في اللبائن.


Article
Effect of Swim Up Techniques on Sperm Motility and DNA Integrity Versus Unprepared Semen
تاثير تقنية السباحة الى فوق على حركة النطف البشرية و سلامة الاحماض النووية لهم مقابل عدم التنشيط.

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Abstract

Background: The need for assisted reproduction technologies (ART) for the establishment of pregnancies has steadily increased worldwide. Therefore, it is of vital importance that an efficient sperm preparation technique used for retrieval of high-quality spermatozoa contributes to the creations of high-quality embryos, with high implantation potential.Objective: to study the effect of swim up technique on human sperm motility and DNA integrity.Subject and methods: A prospective study carried on 70 samples of human semen; each sample, divided into 2 parts, one part was prepared by swim-up technique and the other not, and then study sperm motility and DNA integrity in both parts. Sperm DNA integrity was determined using a modified alkaline single cell gel electrophoresis (comet) assay and acridine orange test (AOT), and motility was determined by light microscope.Result(s): the results showed that swim up technique give a significant. increasing in motility percentage and a significant. decreasing in DNA damage (P< 0.05) than unprepared human sperm.Conclusion(s): Swim up technique for sperm preparation is increasing motility and decreasing DNA damage.

الخلفية: ان الحاجة للتقنيات المساعدة على الانجاب لعلاج حالات العقم في تزايد مستمر , لذلك اصبح من الضروري ايجاد التقنيات الافضل للحصولعلى النطف ذات النوعية الجيدة من حيث الحركة وسلامة الاحماض النووية والتي تؤدي الى الحصول على اجنة ذات نوعية جيدة ايضا مع امكانيةحصول نسبة زرع اعلى لهذه الاجنة في الرحم.الغرض: دراسة فعالية تقنية السباحة الى فوق في تنشيط النطف في الحفاظ على سلامة الاحماض النووية حركة النطف البشرية مقابل عدم التنشيط.الموضوع والطرائق: اجريت الدراسة المستقبلية على 70 نموذج من النطف البشرية , وكل نموذج تم تقسيمه الى قسمين , قسم تم تنشيط النطف بطريقةالسباحة الى فوق والاخر بدون تنشيط, ومن ثم تم دراسة سلامة الاحماض النووية والحركة للنطف لكل قسم.النتائج.ان النتائج المستحصلة من هذه الدراسة تبين ان تقنية تنشيط النطف السباحة الى فوق تعطي نتائج افضل ومعنوية ) (P< 0.05 من ناحية حركةالنطف وسلامة الاحماض النووية من عدم تحضيره.الاستنتاجات: ان تقنية السباحة الى فوق لتنشيط النطف قبل اي تقنية من التقنيات المساعدة على الانجاب من الممكن ان تعطي نتائج افضل بما ان هذهالتقنية تزيد نسبة حركة النطف وتقلل نسبة تحطم الاحماض النووية.


Article
SPERM MOTILITY CONCENTRATION MORPHOLOGY AND PREGNANCY OUTCOME POST-VARICOCELLECTOME IN DUHOK
حركة النطف، اشكالها ونسب حدوث الحمل والاخصاب بعد عملية اصلاح دوالي الخصية في دهوك

Author: KHALIS S. AMMO خالص صبري عمو
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2012 Volume: 6 Issue: 2 Pages: 21-28
Publisher: University of Dohuk جامعة دهوك

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Abstract

Background and objectives Varicocele is a dilatation and tortuousity of pampiniform plexus. It is a very common condition present in 15% of the general male population and 40% of men evaluated for primary infertility and 80% of men with secondary infertility. A varicocele develops because of defective valves that normally allow blood to flow away from the testicle toward the abdomen. The improvement of seminal parameters and rate of pregnancy after varicocele correction had been reported by several investigators in clinical series. The aim of this study is to evaluate the sperm motility, concentration, morphology and pregnancy rate post-varicocelectomy.Methods A retrospective study conducted on 160 male patients who underwent surgery for correction of varicocele for the period between January 2005 to January 2011. All the included patients had primary infertility. Pre and post operative evaluation was held byfrequent seminal fluid analysis. The place of the study was Azadi teaching hospital.Results The improvement in the sperm concentration observed in 80 patients (50%) at 3months, 112 patients (70%) at 6 months, and 128 patients (80%) at 12 months. The sperm motility were improved in 64 patients (40%) at 3 months , 80 patients (50%) at 6 months and 112 patient (70%) at the 12 months .While the improvement in the sperm morphology was seen in 56 patients (35%) at 3 months , 80 patients (50%) at 6 months and 104 patients (65%) at the 12 months. Accordingly the best results obtained after 12 months and the greatest changes were in the sperm concentrations (80%) followed by motility (70%) and morphology(65%). On the other hand the pregnancy rate was 8.1% at 3 months, 21.8% at 6 months and 30% after 12 months.Conclusions We conclude that the palpable varicocele had a bad effect on the sperm parameters , the repair of varicocele improved these parameters, and in the same time it improves the rate of spontaneous pregnancy but not to the same extend as the improvementsin the sperm parameters.

خلفیة واهداف البحث: دوالي الخصیة هو توسع والتواء یحصل في شبكة أوردة الخصیة وهي حالة شائعة تصل نسبتها إلى 15 % من عامة الذكور و 40 % من نسبة الذكور الذین یعانون من العقم الأولي و 80 % من أولائك الذین یعانون من العقم الثانوي. حدوث دوالي الخصیة هو نتیجة خلل في عمل الصمامات الموجودة في الأوردة الناقلة للدم من الخصیة باتجاه البطن. التحسن في نسب وحركة وأشكال النطف بعد عملیة إصلاح الدوالي أخذت اهتمام الكثیر من الباحثین.الهدف من الد ا رسة هو د ا رسة وتقییم نسب وحركة وأشكال النطف مع د ا رسة نسب حدوث الحمل بعد عملیة إصلاح دوالي الخصیة.طرق البحث: هي د ا رسة مستقبلیة أجریت على 160 مریض. أجریت لهم عملیة دوالي الخصیة من الفترة كانون الثاني من العام 2005 ولغایة كانون الثاني 2011 . جمیع المرضى الذین شملوا بالد ا رسة كانوا یعانون من العقم الأولي. سلسلة من الفحص ألمختبري للسائل المنوي اجري لجمیع المرضى قبل وبعد العملیة. مكان الد ا رسة والعمل كان مستشفى أ ا زدي التعلیمي في دهوك.النتائج: كان التحسن في نسب النطف 50 % بعد ثلاثة أشهر من العملیة و 70 % بعد ( 6)أشهر و 80 % بعد 12 شه ا ر.50 % , و 70 % بعد 12, 6, 3 شه ا ر على التوالي. من جهة أخرى كانت , % أما التحسن في حركة النطف فكان 40 21.8 % و 30 % بعد 12،6،3 شه ا ر من العملیة. ،% نتائج نسبة حدوث الحمل 8.1 الاستنتاجات: دوالي الخصیة له تأثی ا رت سیئة على حركة ونسب وإشكال النطف، وعملیة إصلاح الدوالي لها فائدة لكل من نسب وحركة وأشكال النطف وكذلك على نسبة حصول الحمل ولكن بدرجة أقل.


Article
Effect of stress on semen quality in a population of infertile human

Author: Dr. Edress M. Ameen
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2009 Volume: 12 Issue: 1 Pages: 167-174
Publisher: University of Kufa جامعة الكوفة

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Abstract

The aim of the present study was to assess the relationships between the stress which results from the work and human semen quality. The work stress which was studied includes two types; quality stress (easy and moderate to hard performing work/day) and quantity stress (number of hours performing work/day. Results are elaborated by using independent t-test statistical method. From fifty two men which has an easy and moderate work and forty eight men with daily hard job, we determined significant negative (p<0.05) influence of stress on sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability. Quantity stress showed that a men with 7-10 hrs performing job/day has a significant (p<0.05) lower; sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability compare with 4-6 hrs performing job/daily. No significant differences were recorded between quality and quantity stress in which means that both types of stress has the same effect on semen quality.

إن هدف الدراسة الحالية هو تقييم العلاقة بين الجهد الناتج من العمل و نوعية السائل المنوي في الإنسان. تم دراسة نوعين من جهد العمل هي ، الجهد النوعي (أداء عمل سهل و معتدل إلى شاق/ يوم) والجهد الكمي (عدد ساعات العمل المنجزة / يوم). تم إجراء التحليل الإحصائي باستخدام اختبار t . تم الحصول على تأثيرات سلبية معنوية (p<0.05) للجهد على تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و كذلك النسبة المئوية للنطف الحية عند استخدام اثنتان و خمسون رجلا ينجزون عملا سهلا إلى معتدل و ثمانية و أربعون رجلا يؤدون عملا شاقا. أظهرت نتائج الجهد الكمي بان الرجال الذين يعملون لمدة 7-10 ساعات في اليوم لديهم نسبة اقل معنويا (p<0.05) من تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و النسبة المئوية للنطف الحية مقارنة مع الذين يعملون لمدة 4-6 ساعات في اليوم. لم تسجل أية فروقات معنوية مابين الجهد النوعي والكمي و هذا يدل على ان لكلا النوعين من الجهد نفس التأثيرات على نوعية السائل المنوي.


Article
Zinc Sulfate Improves Progressive Sperm Motility in Subfertile Men with Abnormal Sperm Agglutination

Author: Dr. Abdulaali H. Salman,
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2009 Volume: 2 no.6, 7 Issue: 4 Pages: 463-471
Publisher: Kerbala University جامعة كربلاء

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Abstract

back ground: Agglutination of spermatozoa means that motile spermatozoa stick to eachother in the ejaculate. It can be due to infection or antibodies that react with sperm. Spermagglutination has a negative impact on fertility due to impeding of sperm function, inhibitionof fertilization and implantation. Zinc is an essential trace element for male sex function. It is acofactor in more than 300 enzymes influencing various organ functions. Zinc found in seminal fluid,increase sperm count and motility and blood testosterone levels. Zinc had direct effects on theproduction, maturation and function of leucocytes and can modulate antibody production.Objective: The objective of this study to assess the effect of zinc sulfate treatment on spermagglutination among subfertile patients with asthenospermia.Design: Prospective study.Setting: Infertility clinic and assisted reproduction unit at the Institute of Embryo Research andInfertility Treatment, Al-Nahrain University.Patients: Fifty - three subfertile male patients.Materials and Methods: Semen analysis was performed before zinc treatment using direct slideexamination for fifty - three infertile patients. A daily dose of 440 mg of zinc sulfatesupplementation was orally administered for a period of two weeks, three weeks, four weeks, andsix weeks. After each period semen samples were obtained by masturbation after a recommendedperiod of 3-5 days of sexual abstinence, and evaluated for determinations of semen volume, pH,concentration, motility, normal morphology, total progressive motile sperm count, round cells, andsperm agglutination, according to WHO recommendation.Results: subfertile men demonstrated a significant (P< 0.05) decrease in round cells /HPF after thirdweek of zinc treatment. Sperm agglutination was significantly (P< 0.01) decreased after second,third, fourth week, and (P< 0.02) at six weeks of zinc treatment compared to sperm agglutinationbefore treatment. Also the study showed significant (P< 0.05) improvement in progressive spermmotility [grade –A after three week and grade –B after three and four week of zinc treatment],significant (P< 0.01) increase of total progressive motile sperm count /ejaculate, and ofmorphologically normal sperm after third week of oral zinc sulfate supplementation.Conclusions: It was concluded from the results of the work that the oral zinc sulfatesupplementation is effective in decreasing of abnormal sperm agglutination and round cells, withsubsequent improvement of progressive sperm motility.


Article
Changes in seminal antioxidant defenses in Iraqi patients with unilateral and bilateral varicocele

Author: Issam Salman Al-Azzawi
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2014 Volume: 13 Issue: 1 Pages: 1-7
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Background : The exact pathogenic mechanism by which varicoceles cause impaired testicular function remains poorly understood . One of the possible mechanisms is the generation of seminal oxidative stress. In addition, the impact of bilateral varicocele on spermatogenesis is still poorly studied . Aims : To study the changes in seminal antioxidant defenses in relation to unilateral and bilateral varicocele, and to correlate them with changes in sperm concentration and total motility. Patients and methods : A prospective, randomized, controlled study , whereby 60 semen samples were collected and analyzed. Twenty samples were from healthy subjects , having no varicocele ( control group ), 20 samples from patients with unilateral varicocele and 20 samples from patients with bilateral varicocele . For each semen sample, measurement of the seminal concentration of the antioxidants ( Albumin, Glutathione peroxidase and Superoxide dismutase ) was done and the seminal concentration of the oxidative stress marker, Malondialdehyde. In addition sperm concentration and total motility were also estimated. Comparison of the results in the 3 groups was done . Results : Measurement of semen parameters revealed a drop in the mean value of the 3 antioxidants and an increase in the mean value of Malondialdehyde, which was statistically significant in the 2 varicocele groups in comparison with control, and in bilateral varicocele in comparison with unilateral varicocele . Another drop was in the mean sperm concentration and mean percentage of sperm total motility, which was statistically significant in the 2 varicocele groups in comparison with control, but was statistically not significant between the 2 varicocele groups . Conclusions : Bilateral varicocele causes more significant drop in seminal antioxidant defenses than unilateral varicocele which seems to add a more negative impact on spermatogenesis .


Article
Role of Clomiphene Citrate and Tamoxiphene Citrate in Mice Sperm Motility

Authors: Raeʼd Abdalelah Abbas --- Muhammad-Baqir M-R. Fakhrildin --- Mohammad Oda Salman
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 2 Pages: 1-5
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Both clomiphene citrate and tamoxiphene citrate, as an anti estrogen, is the mostcommon prescribed medication for male infertility.In humans, ant estrogens interferewith the normal negative feedback of sex steroids at the hypothalamus and pituitary,thereby increasing endogenous gonadotropin releasing hormone (GnRH) secretionfrom the hypothalamus and follicle stimulating hormone (FSH) and luteinizinghormone (LH) secretion directly from the pituitary.Objective:The objective of this study was to investigate the effect of clomiphene citrate (CC)and tamoxiphene citrate (TC) administered to male mice on quality of sperm motilityand activity.Materials and Methods:Ninety six male mice were divided into two major groups, and every one of thesegroups was subdivided into three minor groups involving control and two treatedgroups. In the CC study, 48 mice were administrated orally 0, 0.0125 mg and 0.025mg as control (G 1), low dose (G 2) and high dose (G 3) for 42 days. While, in theTC study, 48 mice were administrated orally 0, 0.01 mg and 0.02 mg as control (G1), low dose (G 2) and high dose (G 3) for 42 days. Then, the male mice weresacrificed, both vas deferens were collected and assessment sperm motility andactivity.Results: The results of present study appeared that the use high dose of CC or TCsignificantly increment (P<0.05) sperm motility and activity as compared to controlgroups.Conclusions:Based on the results of this study, it can be concluded that the administration of highdose of either CC or TC increases sperm motility and activity.


Article
Relationship between The Addition of Glutathione to Sperm Freezing Medium and Cryosurvival Rate of Sperm Motility and Viability in Asthenozoospermic Patients
العلاقة بين إضافة الكلوتاثايون الى وسط تجميد النطف ومعدل الحركة والعيوشية النطفية بعد النجاة من التجميد في مرضى وهن النطف

Author: Hisham Q. Mohammad Al-Nowainy هشام قاسم محمد
Journal: kufa Journal for Nursing sciences مجلة الكوفة لعلوم التمريض ISSN: 22234055 Year: 2017 Volume: 7 Issue: 2 Pages: 1-10
Publisher: University of Kufa جامعة الكوفة

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Abstract

Objective: This study aimed to determine the relationship between the addition of Glutathione to sperm freezing medium and cryosurvival rate of sperm motility and viability in asthenozoospermic patients.Methodology: fifty nine semen samples of asthenozoospermic patients were obtained from the male partner of infertile couples attending the fertility center of al-sadder medical teaching city in Al-Najaf during the period 1st October 2016 until 1st March 2017.Each semen sample was divided into four equal parts, volume of each part is 0.5 ml: first part (before activation), second part (after activation), third part (cryopreservation with sperm freezing medium for one month), and fourth part (cryopreservation with sperm freezing medium plus glutathione for one month ) .Sperm concentration , sperm progressive motility, sperm viability and sperm normal morphology were counted in first two parts, cryosurvival rate of sperm motility, viability was counted in third and fourth parts .Statistical analysis of the study performed by using Independent t-test and paired test to assess the differences between two groups .Results: After sperm activation by using the swim up technique ,the study results showed significant increase (P < 0.01) of progressive sperm motility, normal sperm morphology and sperm viability, while there were a significant decrease (P < 0.01) of sperm concentration. cryosurvival rate of sperm motility and viability were significant decrease (p < 0.01) after cryopreservation and thawing processes while the results were revealed significant improvement in cryosurvival rate of sperm motility, and viability ( p < 0.01 ) after the addition of glutathione to the sperm freezing medium.Conclusion: it can conclude from this study that addition of glutathione to sperm freeze medium improves. (p < 0.01) cryosurvival rate of sperm motility and viability during cryopreservation and thawing processes .Recommendations: the study recommended to addition 50 microliter of glutathione to sperm freezing media in concentration 1 mM .Keyword: Glutathione, sperm freezing medium, cryosurvival rate, sperm motility, viability, asthenozoospermic .

الهدف: تهدف الدراسة إلى تحديدالعلاقة بين إضافة الكلوتاثايون الى وسط تجميد النطف ومعدل الحركة والعيوشية النطفية بعد النجاة من التجميد في مرضى وهن النطف.المنهجية: تم الحصول على تسعة و خمسين عينة من السائل المنوي من مرضى وهن النطف من الشريك الذكر للأزواج العقيمين الذين يراجعون مركز الخصوبة في مدينة الصدر الطبية التعليمية في النجف وأمتدت الدراسة من المدة(1تشرين الاول 2016 حتى 1 اذار 2017). تم تقسيم كل عينة من السائل المنوي إلى أربعة أجزاء متساوية , حجم كل واحدة 0,5 مل: الجزء الأول (قبل التنشيط)، الجزء الثاني (بعد التنشيط)، الجزءالثالث (جمد بإستخدام وسط تجميد النطف لمدة شهر واحد)، والجزء الرابع (جمد بإستخدام وسط تجميد النطف مضافا اليه الكلوتاثايون لمدة شهر واحد). تركيز النطف، حركة النطف التقدمية ، و المظهر الطبيعي للنطف و العيوشية تم حسابها في كل جزء,ومعدل الحركة والعيوشية بعد النجاة من التجميد، تم حسابها في الجزء الثالث والرابع.تم إجراء التحليل الإحصائي للدراسة بإستخدام إختبارt المستقل وإختبار t المقارن لتقييم الفروق بين المجموعتين.النتائج: بعد تنشيط النطف بإستخدام تقنية السباحة ، أظهرت نتائج الدراسة زيادة معنوية (p < 0.01) في حركة النطف التقدمية، و المظهر الطبيعي للنطف وعيوشية النطف, في حين كان هناك إنخفاض معنوي(p < 0.01) في تركيز النطف.أما معدل الحركة والعيوشية بعد النجاة من التجميد إنخفضت بشكل معنوي (p < 0.01) بعد عمليات التجميد والإذابة , بينما بينت النتائج تحسنا معنويا (p < 0.01) في معدل الحركة والعيوشية النطفية بعد النجاةمنالتجميد بعد إضافة الكلوتاثايون الى وسط تجميد النطف .الإستنتاج: يمكن أن نستنتج من هذه الدراسة أن إضافة الكلوتاثايون إلى وسط تجميد النطف حسن(p < 0.01) معدل الحركة والعيوشية النطفية بعد النجاة من التجميد خلال عمليات التجميد والإذابة .التوصيات : أوصت الدراسة بإضافة 50 مايكرو مول من الكلوتاثايون إلى وسط تجميد النطف بتركيز 1 ميلي مولار.

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