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Article
Effect of stress on semen quality in a population of infertile human

Author: Dr. Edress M. Ameen
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2009 Volume: 12 Issue: 1 Pages: 167-174
Publisher: University of Kufa جامعة الكوفة

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Abstract

The aim of the present study was to assess the relationships between the stress which results from the work and human semen quality. The work stress which was studied includes two types; quality stress (easy and moderate to hard performing work/day) and quantity stress (number of hours performing work/day. Results are elaborated by using independent t-test statistical method. From fifty two men which has an easy and moderate work and forty eight men with daily hard job, we determined significant negative (p<0.05) influence of stress on sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability. Quantity stress showed that a men with 7-10 hrs performing job/day has a significant (p<0.05) lower; sperm concentration, sperm grade activity, percentage of sperm motility, and percentage of sperm viability compare with 4-6 hrs performing job/daily. No significant differences were recorded between quality and quantity stress in which means that both types of stress has the same effect on semen quality.

إن هدف الدراسة الحالية هو تقييم العلاقة بين الجهد الناتج من العمل و نوعية السائل المنوي في الإنسان. تم دراسة نوعين من جهد العمل هي ، الجهد النوعي (أداء عمل سهل و معتدل إلى شاق/ يوم) والجهد الكمي (عدد ساعات العمل المنجزة / يوم). تم إجراء التحليل الإحصائي باستخدام اختبار t . تم الحصول على تأثيرات سلبية معنوية (p<0.05) للجهد على تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و كذلك النسبة المئوية للنطف الحية عند استخدام اثنتان و خمسون رجلا ينجزون عملا سهلا إلى معتدل و ثمانية و أربعون رجلا يؤدون عملا شاقا. أظهرت نتائج الجهد الكمي بان الرجال الذين يعملون لمدة 7-10 ساعات في اليوم لديهم نسبة اقل معنويا (p<0.05) من تركيز النطف و درجة نشاط النطف و النسبة المئوية للنطف المتحركة ، و النسبة المئوية للنطف الحية مقارنة مع الذين يعملون لمدة 4-6 ساعات في اليوم. لم تسجل أية فروقات معنوية مابين الجهد النوعي والكمي و هذا يدل على ان لكلا النوعين من الجهد نفس التأثيرات على نوعية السائل المنوي.


Article
Immediate and Late Effect of in Vitro Low Level Helium-Neon Laser Irradiation on Human Sperm Activity

Author: Ghassan Thabet Saeed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2017 Volume: 16 Issue: 1 Pages: 79-85
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Sperm motility is essential in fertilization because non active or mal active sperms never reach the ovum, but the progress in the assisted reproductive techniques makes it easy for doctors to select viable sperm to be used in intracytoplasmic sperm injection thus the differentiation between viable and non-viable sperms is very important especially in sever asthenospermic samples.OBJECTIVE:To evaluate low level Helium Neon laser irradiation of the whole seminal sample as a method to activate mal or non-active sperms and to study and follow up the late effect of laser irradiation on the seminal samples.MATERIALS AND METHODS: 100 samples were included in this study from patients attending seminal fluid examination lab. After digital seminal fluid examination, samples were divided in 2 parts, the first was incubated for 120min at 37C and the other was irradiated with 623.8 wave length Helium Neon laser and an output power of 2mW at 1cm distance for 10min and then examined digitally after irradiation then incubated with the first part to 120min also to be examined again with the first part.RESULTS: It was found that there is a significant increase in the progressive motile sperm percentage in the laser irradiated samples from about 33.6% to 41.6% (p<0.005) and also decrease in the percentage of the immotile sperms from about 44.1% to 34.4% (p<0.001). While after 120min incubation, active sperms percentage in the irradiated sample was about 43% which is higher than the non-treated part (31.6%; p<0.01) that showed also higher percentage of immotile sperms (52.8%) against (39.9%) in the treated part.CONCLUSION: Helium Neon laser irradiation to the whole seminal sample increase the sperms activity and decrease the percentage of immotile sperms which mean that it stimulates viable but immotile sperm to move. And also, sperms in laser irradiated samples preserved their increased activity for more than 2hours after laser irradiation.

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