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Article
Identification of Streptococcus mutans from Human Dental Plaque and Dental Caries Using 16SrRNA Gene
تشخيص بكتريا Streptococcus mutans من عينات تسوس الاسنان واللعاب باستخدام جين 16SrRNA

Authors: Adhraa S. Flayyih عذراء صالح فليح --- Hayfa H. Hassani هيفاء هادي حساني --- Mohammed H. Wali محمد حسين والي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2016 Volume: 57 Issue: 1C Pages: 552-557
Publisher: Baghdad University جامعة بغداد

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Abstract

One hundred and sixty samples from saliva and dental plaque were sellected from patients with caries active at ages from (4-65) years from Umm Qasr Primary School and Al-Ameria Specialist Dental Center in Baghdad. 15 isolates belong to Streptococcus mutans were identified by biochemical tests and Vitek 2 compact system while 22 isolates identified by using Polymerase Chain Reaction ﴾PCR﴿ techniques and sequencing of 16SrRNA with 120 bp by using 16SrRNA the result confermed that these isolates were belong to S.mutans

جمعت مائة وستون عينة من اللعاب و الاسنان المتسوسة من مرضى يعانون من تسوس الاسنان باعمارمختلفة تراوحت بين (4-65) سنة عند مراجعتهم مركز العامرية التخصصي لطب الاسنان ومن طلبة مدرسة ام قصر الابتدائية , شخصت خمسة عشر عزلة بانها S. mutans بالاختبارات الكيمو حيوية ونظام الفايتك -2 بينما 22 عزلة شخصت باستخدام تقنية سلسلة تفاعل البلمرة الPCR وجين 16SrRNA بحجم120 زوج قاعدة واثبتت النتائج ان هذه العزلات تعود الى S.mutans.


Article
Plasmid Profile of Locally Isolated Streptococcus mutans in Relation with Mutacin Production.

Author: Mohsan H. Risan , Hameed M. Jasim, Aya R. Salh
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 2 Pages: 28-37
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study was aimed to isolate and identify Streptococcus mutans from dental caries, and ability of bacterial isolates for mutacin production and selecting the efficient producer isolate and studying plasmid profile for the selected isolate and its role in mutacin production. In order to isolate Streptococcus mutans, 80 swab samples were collected from dental caries of patients attends Al-Dora health center and Al-Zewiya health center in Baghdad city. From these samples 80 bacterial isolates were obtained. Results of identification depending on morphological, cultural and biochemical tests showed that ten of these isolates were belonged to Streptococcus mutans, then results of identification were confirmed by using VITEK-Π identification system. All isolates were screened to examine their ability in mutacin production. Plasmid profile of S. mutans S2 was studied to investigate its role in mutacin production. Results showed that S. mutans S2 has only one plasmid DNA band. This plasmid was not responsible for mutacin production according to the results of curing experiment by using interchalating ethidium bromide dye in a concentration of 800 μg/ml. Cured cells were still have the ability of mutacin production which indicated that this trait is chromosomally encoded.


Article
Antimicrobial effect of propolis on Streptococcus mutans

Author: Ghada Y ABDUL-RAHMAN
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2002 Volume: 2 Issue: SpIss Pages: 299-303
Publisher: Mosul University جامعة الموصل

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Abstract

We choose two types of propolis extracts; watery and ethanolic; to estimate their activity against the most cariogenic microorganism; Streptococcus mutans, and evaluate this activity in vitro compared with chlorohexidine and tetracycline as negative controls, using the turbidity method, Both watery extract of propolis - WEP and ethanolic extract of propolis - EEP - show good inhibitory effect with significant variance compared with chlorohexidine, which also had better effect than tetracycline against Streptococcus mutans. The WEP at (1%) was the best antimicrobial solution in this study.


Article
Dental Caries and Salivary Streptococcus Mutans in Relation to Primary and Permanent Dentition

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Background : Streptococcus mutans has been implicated as a principle microbial agent in the pathogenesis of dental caries. The aim of this study was to compare caries experience and salivary Streptococcus mutans count among groups of children having primary dentition and adults having permanent dentition.Materials and Methods: The sample consisted of 100 children with an age of 4 – 5 years old and 100 adults with an age of 21 – 22 years old in Baghdad city. Dental caries was examined for the whole dentition and bacteriological analysis was done for the salivary sample to estimate the count of Streptococcus mutans in saliva.Results: Caries experience of adults was significantly higher than that of children while salivary S.mutans count was significantly higher among children than the adults. Positive correlation of S. mutans with decayed surface was stronger than that with (dmfs/DMFS) for both children and adults.Conclusion : S. mutans count was significantly higher among children than adults and caries experienc of permanent teeth was significantly higher than that of primary teeth.


Article
Salivary Streptococcus mutans and dental caries in relation to nutritional status among 6 years old children

Author: Wesal A. Al-Obaidi B.D.S., M.Sc. * د. وصال العبيدي
Journal: MUSTANSIRIA DENTAL JOURNAL مجلة المستنصرية لطب الاسنان ISSN: 18138500 Year: 2006 Volume: 3 Issue: 4 Pages: 386-390
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Nutritional status is the mirror image of health and one of its mostaccurate indicators. Normal salivary gland functions are essential for maintenanceof oral health. Salivary flow rate and composition which could be influenced bynutritional status. The aim of this study was to investigate the effect of nutritionalstatus on Streptococcus mutans and their relations to dental caries.Materials and methods: Stimulated saliva was collected from 28 children with anage 6 years old. Those randomly selected children were divided into wellnourishedand chronic malnourished children according to their age, weight andheight indicators. Dental caries examination of primary teeth was assayedfollowing the criteria of WHO. Microbiological assessment of Streptococcusmutans was done after measuring the salivary flow rate.Results: No significant differences were found between well-nourished and chronicmalnourished children in caries experience. Chronic malnourished children had asignificant lower salivary flow rate and higher mean counts of salivaryStreptococcus mutans than that of well-nourished children. Significantcorrelations were found between nutritional status with flow rate andStreptococcus mutans.Conclusion: Early identification of malnourished children will lower the prevalenceof dental caries and enhance their quality of life.


Article
Comparison between severe haemophilic A and healthy children in Streptococcus mutans, oral Lactobacilli and Candida albicans counts

Authors: Zainab A. AlDhaher زينب الظاهر --- Maha F. Almelan --- Luma M. Alhadi لمى الهادي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2012 Volume: 24 Issue: 3 Pages: 149-153
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Haemophilia is a common hereditary hemorrhagic disorder, however little is known about the oralmicroflora of hemophilic patients.The purpose of this research is to compare between the viable count of Streptococcus mutans, oral Lactobacilli andCandida albicans from saliva of children suffering from haemophilia A aged (6-12) years and the viable count of thesame microorganisms isolated from saliva of healthy subject (healthy supject group) aged (6-12) years.Materials and methods: - Saliva samples were collected from 30 children with severe Haemophilia A (patients groupand 30 healthy children (healthy subject group). Microbial counts of Streptococcus mutans, oral Lactobacilli andCandida albicans were recorded for each group by using colony counter and expressed as colony forming unitmultiplied by the dilution factor per millimeter saliva (CFU/ml).Results: - The present study observed that the viable count of Streptococcus mutans and oral Lactobacilli in patientgroup was higher than the count of the healthy subject group while no significant differences were observedbetween the viable count of Candida albicans in patients group and healthy subject group.Conclusion: - education, prevention and effort among parents and dental professionals can aid in improving the oralhealth of Haemophilia children


Article
Study of The Adherence of St. mutans on Bleached and Fluoridated Tooth Surfaces. (An In Vitro Study)

Author: Sawsan H Al-Jubori
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2013 Volume: 13 Issue: 25 Pages: 116-121
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: To evaluate Streptococcus mutans adherence on bleached enamel surface with (7.5% hydrogen peroxide , 16% carbamide peroxide) with or without topical fluoride theraby. Materials and Meth-ods: Sixty specimens obtained from thirty maxillary premolars extracted for orthodontic reasons, cleaned, polished, and examined for any surface structural damage. The roots were discarded and crowns sectioned in two pieces (mesiodistaly) using diamond separating disc in low speed hand piece. The specimens randomly divided into six groups and exposed to one of the bleaching agents (7.5% hydrogen peroxide , 16% carbamide peroxide) with or without topical fluoride application. The nega-tive control group specimens stored in deionized water. The positive control group specimens stored in artificial saliva. Adherence of Streptococcus mutans count to enamel surfaces was determined bacte-riologically. The data analyzed statistically using one way ANOVA and Tukey test (p≤0.05). Results: there is significant increasing in Streptococcus mutans adherence on enamel surface after bleaching procedure, which was decreased significantly when used fluoride application technique after bleach-ing. Conclusions: Topical fluoride therapy has beneficial effect on reducing the Streptococcus mutans adherence on enamel surface after bleaching regimens which attributed in reducing the possibility of dental caries


Article
Antibacterial Pattern of Lactic Acid Bacteria on Streptococcus Mutans Isolates from Dental Caries

Author: Abduloma Baraka Ali
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 1 Pages: 41-46
Publisher: Babylon University جامعة بابل

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Abstract

Several approaches have been taken in the prevention of dental caries; the main approach is to render enamel surface more resistance to attack of acidogenic bacteria. In this study one hundred dental plague samples with 20-30 mg of plagues were collected. These patient's samples were collected during March to May 2014 from Baghdad dental clinics. Culturing of dental plagues was inoculated on different selective, enrichment and diagnostic media to be obtain pure culture of Strep. mutans. Streptococcus mutans is considered the main cause of destruction of dental tissues, with the incidence 13%. The inhibitory effect of Lactobacillus gasseri against Streptococcus mutans isolates was studied by well-diffusion method on solid media. The results showed that the Lactobacillus gasseri has a highly effects of inhibition zone on the Strep. mutans growth (92.30%) and the inhibition diameters zone were more than 12 mm. Also this effect compared with other antibacterial drugs, which were showed that the isolates had different responses of resistance to Ampicillin (AM) 5(38.4%), Azithromcin (AZM) and Lincomycin (LN) 2(15.4%) for each, Gentamycin (CN) 4(30.7%), so that the Cefotaxim (CTX) antibacterial recorded lowest resistance 1(7.6). Hence, this study reveals the potential of Lactobacillus gasseri as an alternative and complementary treatment medicine for dental caries by inhibiting the growth of Streptococcus mutans. Also these results may provide a basis for alternative therapies to prevent or treatment of dental caries.


Article
Effect of sodium fluoride addition as a disinfectant on some properties of alginate impression material

Authors: Alaa M. Hussian --- Raghdaa K. Jassim
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2015 Volume: 27 Issue: 1 Pages: 70-76
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Impression materials, impression trays, and poured stone cast have been said to be the main source ofcross infection between patients and dentists. However, it was observed that disinfection of the impression is notperformed systematically in routine dental practice. Disinfection of alginates either by immersion or spray techniquewas found to cause dimensional inaccuracies, although with proper disinfection of alginates there were smalldimensional changes. A variety of fluoride releasing products designed for topical use is currently available.Following their use, varied amount of fluoride is systemically absorbed depending on the fluoride concentration andthe manner of its use. The objective of this study was to evaluate the effect of addition (0%, 0.25%,0.5%,1%, 2%, 3%,4% ) of (NaF) as a self-disinfection to alginate impression material powder and its effect on setting time, tear strength,dimensional change and accuracy of alginate impression materials.Materials and methods: A total No. of (700) specimen were used in this study.These specimens were divided into (5)main groups according to the tests used. Each of the four main groups contain (70) specimen divided into 7subgroups (ten specimens to be tested for each (7)concentrations which is composed of six percentages of NaFwithalginate powder (0.25%,0.5%,1%, 2%, 3%, 4% )and one for control (0% NaF) , while the fifth group (Microbiologicaltest) contain 420 specimen were divided between Streptococcus mutans and Candida albicans as (120) specimenfor each one that was subdivided into three subgroups (specimens taken before impression casting, specimensafterpouring of alginate with stone andspecimens of stone were taken from the casts) in which it contain the samesubgroups of the other concentrationsof (NaF) to test its efficacy against (Streptococcus mutans and Candidaalbicans).Results: Tear strength for all experimental impression materials was greater than those of the control products. Therewere no statistically significant differences between the dimensional change tests and also reproduction of detailtest.With regard to setting time of the impressions, statisticallyreduction were seen between the control andexperimental groups of alginate impression materials that contain (NaF). Self-disinfection of alginate impressionmaterial containing NaF showed a significant reduction in the count of cell forming unit of microorganismsimmediately after impressions were made.Conclusion: In cooperation of specific concentrations of (NaF) as a disinfectant in alginate dental impressionproduced a significant reduction of contamination, also they caused non-significant effect on dimensional stability,detail reproduction and significant increase in tear strength. Therefore it is recommended as step in preventing crosscontamination.


Article
Evaluation the Inhibitory Effect of Aloe Vera Aqueous and Alcoholic Extracts on Salivary Streptococcus Mutans In Comparison to Chlorohexidine Gluconate

Author: Maha Jamal Abbas مها جمال عباس
Journal: Tikrit Journal for Dental Sciences مجلة تكريت لعلوم طب الاسنان ISSN: 20731213 Year: 2015 Volume: 3 Issue: 1 Pages: 124-135
Publisher: Tikrit University جامعة تكريت

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Abstract

diabetic properties. The aim of this study (an in vitro study) was to evaluate the effect of Aloe Vera alcoholic and water aqueous extract in different concentration (18%, 28%, 38%, and 48%) on the viable count and inhibition zone of streptococcus mutans and compared with chlorhexidine 2%. In this study the isolation and identification of streptococcus mutans was performed from saliva of individual, aged under 65 years old, these bacteria was isolated, purified and diagnosed according to morphological characteristic features and biochemical test. The effect of Aloe Vera extracts in different concentrations were tested on the isolated species by using agar diffusion technique and the effect of different concentrations of aloe vera on viable count of streptococcus mutans.Results: In vitro experiments, statistically reduction in the viable count of streptococcus mutans was recorded at all concentration of alcoholic Aloe Vera extract and increase in the concentration lead to increase in the reduction of viable count of streptococcus mutans while in this study showed significant reduction in viable count of streptococcus mutans at higher concentration of water extract of Aloe Vera at 38% and 48%.The diameter of inhibition zone of streptococcus mutans were highly significant at all concentration of alcoholic extract in comparison to water extract

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