research centers


Search results: Found 6

Listing 1 - 6 of 6
Sort by

Article
Expression of RANKL by dental cells during eruption of mice teeth

Authors: Lubna K. Jassim لبنى جاسم --- Athraa Y. Al- Hijazi عذراء يحيى الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 1 Pages: 76-81
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background : In order for a tooth to erupt, two obvious requirements are needed. First, there has to be alveolar boneresorption of the bone overlying the crown of the tooth such that an eruption pathway is formed. Second, resorptionof bony crypt and apposition of new one, third, there has to be a biological process that will result in the toothmoving through this eruption pathway.The amniotic sac contains a considerable quantity of stem cells. Theseamniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful for humanapplication. Receptor activator of nuclear factor kappa B ligand (RANKL) is concentrated on bone biology, morespecifically bone metabolism. RANKL plays a vital role in osteoclastogenesis for bone resorption. This study aimed toevaluate the expression of RANKL marker by dental cells during eruption of the teeth.Materials and Methods: : forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cellsin the anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 micefor each period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: The present results localized and identified RANKL marker in 3 areas of developing tooth of the studied groupsincludes overlying, surrounding and apical bone. Positive RANKL with high significant value expressed by osteoclastof overlying bone in Amnion group followed by Control at day 4. In surrounding bone positive expression of RANKLillustrated to be highest in Control followed by Amniotic fluid at day 10.Apical bone shows positive expression ofRANKL in amniotic fluid group and it records to be the highest value in comparison to studied groups at day 10.Conclusion Expression marker RANKL illustrates that amniotic fluid group has a high expression of RANKL in osteoclastsurrounding and apical bone areas while control expressed RANKL in osteoclast of overlying bone. The present resultsopened clinical hopes in dental tissue engineering by application of autologous amniotic fluid and chorion cells

Keywords

RANKL --- tooth eruption


Article
Immunohistochemical study of CD34 in tooth eruption by using amniotic stem cells

Authors: Lubna K. Jassim لبنى جاسم --- Athraa Y. Al- Hijazi عذراء الحجازي
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 47-53
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Tooth eruption is a more general process, however, which includes certain posteruptive toothmovements. There are two fundamental requirements for both tooth eruption to occur:(1) Require soft tissue, intervening between tooth structure and alveolar bone, which plays an important role inregulating the remodeling of adjacent tissues.(2) Require bone turnover that is temporally and spatially regulated to facilitate specific translocations of teeththrough alveolar boneThese amniotic stem cells are multipotent and able to differentiate into various tissues, which may be useful forhuman application and recently it used in many medical branches. CD34 is an endothelial marker that is extensivelyused in immunohistochemistry and most vascular endothelial cells. Expression of the stem cell antigen CD34 is adefining hallmark of hemopoietic stem cells and progenitors. This study aimed to study the expression of CD34 bydental cells involved in tooth eruption after administration of amniotic stem cellMaterials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: Immunohistochemical result revealed positive expression of CD34 in pulp (Vascular, Paravascular),Mesenchymal cell and in the Dental sac of different groups. Coincidence test of expression marker CD34 in variousstudied group shows that Chorion application affected on CD34 expression in pulp while Amniotic fluid affected ondental sac.Conclusion Immunohistochemical study of expression marker CD34 in various studied groups show that chorionapplication affected on CD34 in pulp .While amniotic fluid affected on dental follicle


Article
Histometric Evaluation of Atenolol Effect on Developing Rat Dentition: Experimental Study

Author: Asmaa S Al-Douri
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2012 Volume: 12 Issue: 19 Pages: 153-159
Publisher: Mosul University جامعة الموصل

Loading...
Loading...
Abstract

Aims: To investigate the histometric effect of atenolol drug on the developing dentition of rats. Mate-rials and Methods: The pregnant rats were divided into two main groups; control and atenolol treated groups. The control pregnant rats were given 1 ml distilled water orally from 12 – 18 day of gestation period, after delivery of these rats, thirty newborn rats from six mothers were sacrificed at ages 1, 5, 10 days postnataly (p.n). The experimental group were given 10 mg atenolol powder suspended in 1 ml distilled water orally, daily as a single dose from 12 – 18 day of gestation, thirty newborn rats from each six mothers were sacrificed at ages 1, 5, 10 days postnataly. The specimens were prepared for processing and staining with Hematoxyline and Eosin stains, and then examined by Motic microscope. Histometric measurements were under taken for these specimens by graticule lense. The histometric parameters used are the dentin thickness, mesiodistal dimension of molar teeth, pulp density (cellulari-ty) and eruption rate. Results: The results showed that atenolol affect on the developing rat dentition .This manifested by less dentin thickness, decreased mesiodistal dimension, increase in pulp cellularity and delay in eruption rate of lower incisor tooth in the atenolol treated groups in comparison to the con-trol groups. These observed changes are proceeded or continued as development process until last ex-premintal age 10 p.n. Conclusions: Atenolol drug delay the tooth development and affect it.


Article
Time of Emergence of Permanent Teeth and Impact of Nutritional Status among 4-15 Years Old Children and Teenagers in Basrah City /Iraq

Authors: Hiyam S. Ahmed هيام احمد --- Zeyneb A. Al-Dahan زينب عبد الرضا الدهان
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2016 Volume: 28 Issue: 4 Pages: 134-140
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: The timing of eruption of permanent teeth is of considerable importance to the dental health planning for diagnostic, preventive and therapeutic measures for children and teenagers. The purposes of this study were to determine timing of maxillary and mandibular permanent teeth emergence (except third molars) and to evaluate the effect nutritional status by anthropometric measures on the eruption time of permanent teeth, investigations had been done according to jaw and gender variations.Materials and Methods: This study was conducted among four to fifteen years old children and teenagers from kindergarten and schools in Basrah city in the south region of Iraq. The total sample composed of 1807 children and teenagers that were collected randomly from kindergartens, primary and secondary schools in Basrah city.The data were statistically analyzed by using probit model in order to compute the median 5th and 95th percentile range of emergence. Athropmetric measures of height and weight were used for the purpose of assessment of nutritional status. The indices include: Weight for age, Height for age and Weight for height; each was considered as in term of standard deviation score (Z – score) as primary indicator of underweight, stunting and wasting respectively. The statistical significance of differences in mean of a normally distributed variable (nutritional indices z score) between 2 groups was assessed by independent samples t-test.Results: The results showed significant differences (p<0.05) between the timing of maxillary and mandibular teeth emergence in girls and boys, with earlier emergence in girls , also the mandibular teeth emerge before their maxillary opposing teeth in both sexes except for premolars .The prevalence of malnutrition according to height for age, weight for age, and weight for height nutritional status indicators were found to be 7.4 %, 3.7 % and 1.5% respectively.The results showed that among well-nourished children and teenagers described by height for age nutritional status indicator, most teeth were significantly erupted earlier than stunted except the lateral incisor which erupted earlier in stunted boys than well-nourished boys but the difference was not significantly accepted. The greatest difference of median eruption age of permanent teeth between well -nourished and stunted found in girls in the second molar tooth.Conclusions: Records indicated that the Iraqi children exhibit variation in their times of permanent teeth emergence when compared with other studies, and among well-nourished children and teenaged described by height for age nutritional status indicator, most teeth were significantly erupted earlier than stunted children and teenagers


Article
Enhancement of tooth eruption by using amniotic stem cells (Immunohistochemical study of VEGF marker)

Authors: Athraa Y. Al- Hijazi عذراء الحجازي --- Lubna K. Jassim لبنى جاسم
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 80-88
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: Tooth eruption is a localized process in the jaws which exhibits precise timing and bilateral symmetry.Develop within the jaws and their eruption is a complex infancy process during which they move through bone totheir functional positions within the oral cavity. For species with more than one set of teeth, eruption of the second setalso accomplishes. The key to the successful clinical management of tooth eruption consists of understanding thatthis process consists largely of the local regulation of alveolar bone metabolism to produce bone resorption in thedirection of eruption and shift and formation of bone at the opposite side.The amniotic sac contains a considerablequantity of stem cells. These amniotic stem cells are able to differentiate into various tissues, which used in many field.Vascular endothelial growth factor (VEGF) is an important angiogenic factor reported to induce migration andproliferation of endothelial cells, enhance vascular permeability, and modulate thrombogenicity. VEGF expression incultured cells (smooth muscle cells, macrophages, endothelial cells) is controlled by growth factors and cytokines.The aim of this study was to study the administration of cell molecules of (Chorion, Amnion and Amniotic fluid)around developing mouse tooth and studying the expression of VEGF marker.Materials and Methods: forty eight albino Swiss mice of one day old age injected with isolated amniotic stem cells inthe anterior region of maxilla (incisors area) other 16 mice injected with saline represents control. Sacrifice 4 mice foreach period (4, 7, 10, and 13) day old age. The result were studied histologically and immunohistochemistry.Results: VEGF marker localized and identified in 3 areas; pulp, P.D.L, and Bone. In pulp. The mean value of positiveVEGF expression showed to be highest in Amnion group in comparison to the other studied groups. The marginalmean value of all periods reported to be highest in Amnion groups followed by Chorion group. The period 10 dayshowed highest marginal means value for positive VEGF expression for all groups. In P.D.L. area Amniotic fluid recordsthe highest mean and marginal mean value specifically at day-10 in comparison to other studied groups. In Bonearea Amniotic fluid records the highest mean and marginal mean value among the studied groups followed byChorion group. Period 7-day and 10-day shows high mean value for VEGF expression. Coincidence test for VEGFmarker illustrates to be affected by Amniotic fluid application in P.D.L. and in bone area while Amnion and Chorionapplication showed to be concerned with pulp.Conclusion. It reported that amniotic fluid application affected on expression of VEGF in P.D.L and bone whileamnion and chorion showed to affect on expression of VEGF in pulp.The present study highlighted on clinical andresearcher application of Amniotic fluid and Chorion for supplement of stem cell in dental tissue engineering or evenin other body tissues.


Article
The influence of type I diabetes mellitus on dentition and oral health of children and adolescents attending two diabetic centers in Erbil city
تأثير داء السكري من النوع الأول على طب الأسنان وصحة الفم للأطفال والمراهقين الذين يحضرون مركزين لمرضى السكري في مدينة أربيل

Authors: Vian O. Majeed --- Sazgar M. Saber --- Hemn M. Sarmamy
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2012 Volume: 16 Issue: 3 Pages: 204-212
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Loading...
Loading...
Abstract

Background and objectives: The aim was to determine the effect of type I diabetes melli-tus on eruption time of dentition and to evaluate the dental caries, debris, calculus and gin-gival index in two groups.Methods: Type I diabetic children and adolescents with age range (6-14) years who were attending to Layla Qasim Center for Diabetes and The Diabetic Child Association Center in Erbil city were included in this study from December 2010 to March 2011. The dentition of all participants was examined for eruption. Besides, the DMF/dmf index, debris index (DI), gingival index (GI) and calculus index (CI) were evaluated. Data obtained from each group were compared statistically.Results: When compared to the non-diabetic group, we observed that dental development was as early as (1.6) years in diabetic group. Results also showed that the total mean value of caries experienced (dmft, dmfs,DMFT and DMFS) by the diabetic group was less than that of the control group with significant differences. The gingival index among dia-betic group was higher than the control group. The total mean value of simplified debris index in control group was higher than the diabetic group. In contrast, simplified calculus index was less in diabetic group with a significant difference between them.Conclusion: The findings showed that patients with type I diabetes mellitus had an earlier tooth eruption and plays an important part in the oral health of children.

Listing 1 - 6 of 6
Sort by
Narrow your search

Resource type

article (6)


Language

English (6)


Year
From To Submit

2016 (1)

2013 (3)

2012 (2)