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Genotyping of Human Papilloma Virus Infections and Phenotyping of Tumor Infiltrating Lymphocytes in Iraqi Patients with Uterine Cervical Neoplasia

Author: Majid Mohammed Mahmmod Al-jewari
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2007 Volume: 6 Issue: 4 Pages: 362-373
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Among most basic and clinical investigations for detection and genotyping HPV, only In Situ Hybridization (ISH) is effective in studing the relation of HPV genome to that histopathological entity. Tumor Infiltrating Lymphocytes (TILs) represent the local cellular immune response against both cervical cancer and the associated HPV.OBJECTIVE:This study is aimed to investigate ISH in detection and genotyping of HPV and immunohistochemical (IHC) study in phenotyping of TILs in Iraqi women with different grades of cervical neoplasia.MATERIALS AND METHODS:This retrospective research included a total number of 129 cervical tissue blocks , 64 with invasive and 27 with pre-invasive cervical neoplasia,12 with condylomata acuminata,11 with chronic cervicitis and 15 with apparently- healthy cervices .Molecular detection and genotyping of HPV DNA were performed by using ISH whereas immunophenotyping was done by IHC using monoclonal antibodies for that specific CD markers representing some of TILs.RESULTS:The overall prevalence of HPV DNA in total group of invasive cervical neoplasia was 28.4% , 12 out of 48(25%) with squamous cell carcinoma(SCC) and 8 out of 16(50%) with adenocarcinoma (AC). HPV 16 constituted (58.3%) of HPV- positive invasive cervical SCC , where as HPV 31/33 and lastly HPV 18 constituted (25%) and (16.7%) , respectively .In AC group, HPV 16, 18, 31/33 occupied 50% , 25% , 12.5% of this group, respectively .The prevalence of HPV DNA in pre-invasive cervical neoplasia group was 22.2%. While no case with HPV 18 was detected , HPV 16 and HPV 31/33 genotypes occupied 40% and 60% , respectively .Although CD3+,CD4+and CD8+ lymphocytes showed high mean values , significant differences present only between HPV DNA- positive tissue blocks and control group (p<0.05) . The CD20+and CD56+ lymphocytes showed low mean values.Compared to control group,however,statistical analysis showed significant differences (p<0.05) regarding CD20+ and non-significant regarding CD56+ lymphocytes .CONCLUSION:Using ISH proved more powerful and sensitive tool in revealing precised effects of HPV in cervical neoplasia compared to histopathological examination .High mean values of CD4+ and CD8+ versus low mean values of CD20+ and CD56+ lymphocytes could reflect an important and specific role of these cellular responses against HPV viruses during initiation and progression of HPV-associated cervical cancers.


Article
Localization of Human Cytomegalovirus- Late Gene DNA, Expression of P53 Gene and CD8-Tumor Infiltrating Lymphocytes in Oral Squamous Cell Carcinoma.

Authors: Saad Hasan Mohammed Ali --- Majed Mohammed Mahmood Al Jewari --- Noor Al Huda Ali A.H. Saaed
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 2 Pages: 296-305
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND:Molecular DNA hybridization has confirmed an association of CMV infection with a variety of oralcancers. While cell mediated immunity is most important in controlling primary or reactivated CMVinfection, this virus has one of most effective strategies in oral carcinogenesis via impairment ofstructure and function of P53 protein by interaction with viraloncoproteins. OBJECTIVE:1.To determine the percentage of CMV-infected oral squamous cell carcinoma tissue specimens; 2.To evaluate the histopathological impact of the expression of mutated p53 tumor suppressor gene onCMV- related, as well as CMV-non-infected oral cancer; and 3.To through a light on someimmunological microenvironment of OSCC,by assessing CD8-positive tumorinfiltrating cytotoxicT-lymphocytes. METHODS:This study was designed as retrospective research. A total number of seventy (70) formalin-fixedparaffin-embedded oral tissues were collected; 60 patients with oral squamous cell carcinoma, and 10individuals with apparently-healthy oral tissues. The molecular methods for CMV DNA detection was performed by sensitive version of in situ hybridization, whereas the phenotype of cell surfaceantigen marker, namely CD8+ marker of tumor infiltrating lymphocytes and TP53 protein weredetected via relevant immunohistochemical methods. RESULTS: Well differentiated grade constituted 81.7% of oral squamous cell carcinoma. Positive in situ hybridization reactions for CMV-DNA were observed in 43.3% of the total screened tissues. Thirtythreeout of sixty (55%) oral squamous cell carcinoma showed positive immunohistochemicalreactions indicating P53 over-expression, and 18.3% showed presence of CD8-positive tumorinfiltrating lymphocytes. None of those control group showed positive reaction for CMV-DNA, p53,or CD8 marker.CONCLUSION:The detection of high percentage of cytomegalovirus-DNA in OSCC could mark for a parentral wayof spreading of such important and well-known sexually transmitted infection among Iraqi generalpopulation.The obvious high percentage of mutated p53 over-expression indicates for an importantrole of such genetic events in the oral carcinogenesis.A little role for CD8-positive tumor infiltratinglymphocytes could be played in the immunological microenvironment of OSCC

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