research centers


Search results: Found 6

Listing 1 - 6 of 6
Sort by

Article
Isolation of Uropathogens from Pediatric Associated UTI, with Special Focus on the Detection of Proteus Vulgaris

Author: Basima Q. Hassan AL-Saadi ˡ , Sanaa J. Kadhum1, Seham Hamel Muhaiesenˡ
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2015 Volume: 14 Issue: 1 Pages: 77-84
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Abstract: The study was conducted in AL-Kadhumia hospital for children, AL-Kadhumia city / Baghdad, through period April-July 2014. Mid –stream urine of 75 patients were cultured. Out of these 67(89.33%) patients` urine were positive for bacteriological culture. The distribution of infection was 50.7% infants aged 2-9 months and 46.3% children aged 1-4 years. Total of six bacterial species isolated were E.coli 35(52.2%), Enterobacter spp. 16(23.9%), Proteus spp.11 (14.9%), Klebsialle spp. 3(4.7%), Pseudomonas spp. 1 (1.5 %) and Staphylococcus spp. 1(1.5 %). The isolated organisms were identified based on conventional bacteriological and biochemical analyses and were characterized. PCR technique was used to detect Proteus vulgaris by using species – specific primers Urease C, the results of primer urease C observe 3 isolate were positive. Rapid diagnosis of the pathogen in a clinical sample is always very important.


Article
Antibiotic resistance of urinary tract pathogens and rationale for empirical antibiotic therapy in children with urinary tract infection
المقاومة للمضادات الحيوية من مسببات الأمراض المسالك البولية والأساس المنطقي للعلاج بالمضادات الحيوية التجريبية في الأطفال الذين يعانون من التهاب المسالك البولية

Author: Nazdar Ezzaddin Rasheed Alkhateeb
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2016 Volume: 20 Issue: 3 Pages: 1458-1466
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Loading...
Loading...
Abstract

Background and objective: Increased antimicrobial resistance of urinary tract pathogen is a matter of global public health concern. The purpose of this study was to identify the most common bacteria causing urinary tract infection and detection of antibiotics susceptibility of isolates to evaluate the options for empirical antibiotic therapy in children with urinary tract infection in Erbil city.Methods: This study was conducted in Raparin Teaching Hospital on urine samples culture results over a one year period retrospectively. Hospital microbiology recording book was screened. Throughout the study period, 1622 children suspected to have urinary tract infection were investigated for urine culture. Disc diffusion technique according to clinical and laboratory standards institute (CLSI) was performed to determine antibiotics susceptibility of isolated bacteria species.Results: Of 1622 children with suspected urinary tract infection, 514(31.69%) had a positive bacterial culture that included 104 males and 410 females. The most common isolates were E Coli (54.1 %), Staphylococcus (19.1%) and Proteus (12.5%). The most effective antibiotics against isolated pathogens were imipenem, ciprofloxacin and nitrofurantoin with sensitivity rate 95.2%, 78.8% and 74.1% respectively.Conclusion: This study revealed that E. coli and staphylococcal strains were the most frequent isolated pathogens among our population; empirical antibiotic selection should be based on the knowledge of the local prevalence of bacterial organism and antibiotic sensitivity.


Article
Inhibitory Effect of Parsley (Petroselinum Crispum) Juice Against Some Urinary Pathogens in Vitro

Author: Khalida Kareem Al-Kareemi
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 3 Pages: 336-342
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Information's on the potency of many medical plants against microorganisms are scanty, and in the current wave of antimicrobial resistance against chemotherapeutic drugs, there is a need to search for plants that could be resistance-free and affordable.OBJECTIVE:The objective of this study was to investigate the antibacterial effects of Parsley (Petroselinum crispum) against uro-pathogens and to compare with the effect of some drugs used for the treatment of urinary tract infection caused by different Gram positive and Gram negative bacteria.MATERIALS & METHODS:A total of thirty eight Gram positive and Gram negative bacteria(Escherichia coli ,Proteus sp.,Enterobacter spp.,Pseudomonas aeruginosa, Klebsiella sp. , Staphylococcus aureus) isolated from urine samples of different child patients between first of June 2011 to first of July 2011 admitted to Children Mel fare Teaching Hospital in Baghdad were tested against different dilutions of Parsley (100%,1:1,1:5,1:10:1:15,1:20) to examine the inhibitory activity in vitro ,in addition antibiotic susceptibility test was done.RESULTS:All isolates showed sensitivity to concentrated parsley 100% concentration except Pseudomonas aeruginosa isolates, the highest effect observed on Proteus sp. and Staphylococcus aureus isolates, while inhibitory effect stopped after 1:1 dilution against all isolates, while in antibiotics therapy (29) isolates were resistant to Co-Trimoxazole and Cefotaxime and (30)isolates showed resistance to Tri-imetheprim+clavulanic acid.CONCLUSION:It seems that Parsley has potent antibacterial activity against some uropathogens in vitro.


Article
Antibacterial Susceptibility Of Commonly Encountered Uropathogens In Al-Hussain Teaching Hospital-Thi-Qar Province: A Retrospective Study
دراسة ارتجاعية حول حساسية البكتيريا المسببة لعدوىالقناة البولية للمضادات البكتيرية في مستشفى الحسين التعليمي- محافظة ذي قار

Author: Dr.Rana M. Khalaf د. رنا محسن خلف
Journal: Thi-Qar Medical Journal مجلة ذي قار الطبية ISSN: 19929218 Year: 2010 Volume: 4 Issue: 2 Pages: 74-81
Publisher: Thi-Qar University جامعة ذي قار

Loading...
Loading...
Abstract

ABSTRACT: Background: Antibacterial resistance is an evolving problem which reflects the antibacterial prescription in our community. Such problem has great impact on proper management and consequences of urinary tract infection (UTI)Aim: To determine the relative role of uropathogens and their antibiotic susceptibility pattern in community-acquired urinary tract infection (UTI) in Al-Hussain Teaching Hospital/ Thi-Qar Province.Methodology: A retrospective study was carried out to review urine culture and antibiotic sensitivity testing from symptomatic outpatients visiting Al-Hussain Teaching Hospitals/ Thi-Qar Province over the period from June 2007 to June 2008. Results: Of 236 samples, 124(52.5%) were culture positive. Staphylococci and Escherichia coli were the leading cause of UTI accounting for 75% of all isolates. The rates and roles of other pathogens, including Klebsiella species (11%), Pseudomonas species (8%), Enterobacter, Proteus, and Streptococci were responsible for the remaining 6%. More than 75% of isolated Staphylococci and Escherichia coli were sensitive to nitrofurantoin and amikacin, while more than 60% were sensitive to ciprofloxacin. Sensitivity to commonly prescribed antibacterials as co-trimoxazole, amoxicillin,ampicillin and gentamicin were less. Conclusion: Common uropathogens were highly sensitive to nitrofurantoin, amikacin and to lesser extent to ciprofloxacin, while exhibiting low sensitivity pattern against commonly prescribed antibiotics including co-trimoxazole, amoxicillin, ampicillin, nalidixic acid and gentamicin.

الخلاصة: من اجل تحديد نمط حساسية البكتيريا المسببة لعدوى القناة البولية للمضادات البكتيرية في مستشفى الحسين التعليمي- محافظة ذي قار تم اجراء دراسة ارتجاعية لنتائج زرع البول و فحوصات حساسية المضادات البكتيرية للمرضى المراجعين للعيادة الاستشارية في المستشفى المذكور ابتداءا من حزيران 2007 و لغاية حزيران 2008.من بين 236 عينة وجد ان 124 (52.5%) عينة موجبة للنمو البكتيري, الستافيلوكوكس و اي كولاي كانت السبب الرئيسي لعدوى القناة البولية(75%) من بين العينات الموجبة, فيما وجد ان الكلبسيلا مسؤلة عن 11%, سودوموناس 8%, اما الانتيروبكتر والبروتيس و الستربتوكوكس فقد شخصت في 6% من العينات.البكتيريا المرضية الاكثر شيوعا اظهرت حساسية معتد بها لكل من الناتروفيورانتوين و الاميكاسين وبدرجة اقل للسبروفلوكساسين. بينما اظهرت حساسية محدودة للمضادات البكتيرية الشائعة الاستعمال و هي الكوترايماكسازول, اموكسسلين, امبسلين, جنتامايسين و نالدكسك اسد.


Article
The effect of climate temperature and daily water intake on the diversity of uropathogens causing urinary tract infections in adult hospital patients
تأثير حرارة المناخ وشرب الماء على تنوع الجراثيم المسببة لتهاب المجاري البولية في مرضى المستشفيات

Loading...
Loading...
Abstract

Background: The most frequent bacterium causing urinary tract infection is Escherichia coli followed by Staphylococcus saprophytic. These infections are more prevalent among pregnant women, particularly in summer, due to dehydration and insufficient uptake of drinking water.Objective: To assess the effect of climate temperature on diversity of uropathogens causing UTIs, and investigate whether infection increases during specific months.Patients and Methods: Seven hundred forty one patients aged 20 years or above in General Zakho Hospital was investigated for urinary tract infection status from January to December 2015, and the daily drinking water uptake was assessed. The monthly climate temperatures were analyzed. Urine samples were collected using mid -stream method, and the samples were cultured to isolate uropathogens identified by biochemical tests.Results: The results indicate that 494 (66.66%) out of 741 patients were infected with UTIs, with seasonal trends of a peak plateau from June to July and a sharp decline after September. The percentage of UTI was 71% among patients who drank less than 2 liters of water per day, and 60% for other infections. Pregnant women had significantly higher prevalence of uropathogens due to the physiological changes during pregnancy. E.coli was the most common isolated pathogen (51.7%) in male and female patients.Conclusion: The results support evidence suggesting that dehydration due to low water intake, especially in summer, leads to low urine output, which increases the occurrence of urinary tract infection.

خلفية الدراسة: اكثر البكتريا المسببة لتهاب المجاري البولية هي بكتريا القولون ويليها بكتريا العنقودية. الاصابات اكثر انتشارا في النساء الحوامل وخاصة في فصل الصيف بسبب الجفاف وعدم شرب المياه بكميات كافية.الهدف من الدراسة: لتحديد مدى تأثير حرارة المناخ على تنوع الجراثيم المسببة لتهاب المجاري البولية و التحقق فيماذا كانت هذه الامراض تزداد في فصول معينة.المرضى والطرائق العمل: اجريت الفحوصات على 741 مريض بعمر 20 سنة فاكثر في مستشفى زاخو العام للتاكد من اصابتهم باللتهاب المجاري البولية. وتم تقدير كمية مياه الشرب و تحليل درجات الحرارة شهريا. عينات الادرار تم جمعها بتقنية البول الوسطي وتم زرعها على اوساط زرعيه لعزل وتشخيص الجراثيم بالفحوصات الكيمياوية الحيوية. النتائج: اظهرت النتائج ان 494(66.66%) من مجموع 741 مريض لديهم اصابات بالتهاب المجاري البولية, وكان الاصابات في ذروتها في شهر حزيران و تموز وقلة الاصابات بعد شهر ايلول. واظهرت النتائج ان نسبة اصابات اللتهاب المجاري البولية كانت (71%) في المرضى الذين شربوا أقل من 2 لتر من الماء يوميا و(60%) للامراض الاخرى. واظهرت النساء الحوامل نسبة عالية من الجراثيم المسببة لتهاب المجاري البولية بسبب التغيرات الفسيولوجية اثناء الحمل. وكانت بكتريا القولون المعزولة الأكثر شيوعا ( 51.7 ٪ ) في المرضى الذكور والإناث. الاستنتاجات: النتائج تدعم الحقائق التي تنص على ان الجفاف بسبب قلة شرب المياه وخاصة في فصل الصيف يؤدي الى قلة البول وبالتالي زيادة الاصابة بالتهاب المجاري البولية.


Article
Antibiotic Susceptibility of Bacteria Isolated from Patients with Diabetes Mellitus and Recurrent Urinary Tract Infections in Babylon Province, Iraq

Author: Mohammed Hassan Ali Alhamdany
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2018 Volume: 15 Issue: 1 Pages: 63-68
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Background: The emergence of the antibiotic resistance in the treatment of UTIs is a serious health problem, especially in the developingcountries where there is high level of ignorance, poverty, and bad hygienic practices, Objective: The aim of this study was to detect the typesof bacteria and their antibiotic sensitivity in patients with diabetes mellitus with recurrent urinary tract infections and compare between typesof bacteria in patients with the previous admission to hospital from those who are not. Materials and Methods: This cross-sectional studywas conducted on eighty patients with diabetes mellitus and recurrent urinary tract infection, those patients who visited the Outpatient Unit inDiabetic and Endocrine Center in Merjan Medical City in Babylon Province. This study was carried out from March 1, 2016 to September 30,2016. General urine examination was done to the patient with urinary tract infection. Urine samples were sent for culture and sensitivity againstdifferent types of antibiotics. Results: Results found that the mean age of the patients was (58.23 ± 14.38) and majority of them (63.7%) werefemale and (52.5%) of them came from rural area. The main bacteria causing urinary tract infection was Escherichia coli in more than 55% ofcases, while the amikacin antibiotic regard as the best antibiotic in the treatment of urinary tract infection in this study with lowest resistancepercentage (3.8%). Based on the history of previous admission to the hospital, there was 57.5% with a history of previous admission, andthere was statistically significant difference in the types of bacteria between the two groups. Conclusion: There was significant difference inbacterial type between patients who previously had hospital admission and those who had not. E. coli was the main bacteria causing UTI inthis study. Amikacin showed the best sensitive drug for bacteria that cause urinary tract infection.

Listing 1 - 6 of 6
Sort by
Narrow your search

Resource type

article (6)


Language

English (4)

Arabic and English (1)


Year
From To Submit

2018 (1)

2016 (2)

2015 (1)

2012 (1)

2010 (1)