research centers


Search results: Found 4

Listing 1 - 4 of 4
Sort by

Article
The association of urinary tract infection with urothe- lial carcinoma
العلاقة بين التهاب المجاري البولية وسرطان النسيج البولي

Author: Yasir M. Abdul-Lateef ياسر مفيد عبد اللطيف
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2015 Volume: 8 Issue: 2 Pages: 129-142
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: In Iraq bladder carcinoma is a major problem according to the results of the Iraqi cancer registry between the years (1991-2006). Cancer of the urinary bladder was the 2nd on the table of the commonest ten cancers in male and it was the 8th commonest cancer in females (1).According to the same registry the main histological types were: Transitional cell carcinoma: 43.82%.Sequamous cell carcinoma: 4.49%. Undifferentiated cell carcinoma: 0.31 %. Adenocarcinoma: 0.92%.Male to female ratio was 3.39:1 the peak age incidence was found in the 70+ years (1). Bilharzial ovals were found in about7%-29% of bladder cancers, which is lower than that in Egypt; 82-90% (2). On the other hand urinary tract infections are among the most common bacterial infections that lead patients to seek medical care. It had been estimated that more than 6 million outpatient visits and 300,000 hospital stays every year are due to urinary tract infections (3). The aim of this study is to nd the association or disassociation between bacterial infection of the urothelium and urothelial carcinoma and if it was associated which type is it: causative or associative.Material and Method: This study was carried out on 105 patients with carcinoma of the bladder (transitional, sequamous), whom were referred to the surgical specialties hospital, medical city for diagnostic transurethral endoscopy during the period between 5th of October 2007 till the 1st of September 2008. History of smoking, previous recurrent urinary tract infection and stone was taken, identi cation of the causative bacteria for their urinary tract infection was done from three samples collected; two urine samples (on admission and post-operatively) and one tissue sample (tumor biopsy).Results: The results showed that: 85.65% of the patients had infections associated with their tumors, the rest 14.35% were negative. These infections were either Escherichia coli, Klebsiella, Proteus, Staphylococcus, Enterobacter, Citrobacter or Mor- ganella spp. Escherichia coli was the most common 73.3% of all the cases. The results also showed that there was a signi cant association between positive urine culture on diagnosis for bacterial growth and urothelial carcinoma, also Escherichia coli was collected from the intracellular compartment by a mechanical approach.Conclusion: Associations between urothelial carcinomas and bacterial growths were studied and showed that urothelial carcinomas were highly associated with bacterial growth especially Escherichia coli. Although Klebsiella, Proteus, Staphylo- coccus, Enterobacter, Citrobacter or Morganella spp., occured in a lower incidence, still had a role in urinary tract infection. Finding Escherichia coli intracellular and its high association with urothelial carcinoma could put these bacteria in the caus- ative section of urothelial carcinoma through producing a recurrent acute infection.

تضمنت الدراسة 105 مريضا يعانون من اورام المثانة البولية والذين راجعوا العيادة الخارجية لمستشفى الجراحات التخصصية في مدينة الطب واحيلوا جراء الفحص التشخيصي للنسيج البولي بالناظور للفترة ما بين الخامس من تشرين ا ول 2007 ولغاية ا ول من ايلول 2008, وكانت اعمارهم تتراوح بين اثنان وعشرين وواحد وسبعين سنة. أُخذت نبذة عن الحالة المرضية, كذلك أُخذت ث ث عينات من كل مريض : عينة من ا درار الموجود بالمثانة البولية من خ ل الناظور, عينة من نسيج الورم السرطاني, وعينة من وسط مجرى ا درار ما بعد العملية الجراحية. أُرسال نصف كمية النسيج السرطاني الى قسم تحليل النسيج المرضي لمعرفة نوع النسيج. استنبات باقي النسيج المرضي وعينتي ا درار التي أُستحصل عليها من خ ل الناظور التشخيصي و عينة وسط مجرى ا درار ما بعد العملية في اغار الدم والماكونكي. لوحظ نمو البكتيريا في )%85.65( من الحا ت وكانت في الغالب سالبة الغرام. كانت نمو أشريكية القولون ))E. coli 73.3%(( من العينات, اما عن الباقي فتراوح بين الكلبسي , البروتيوس, المكورات العنقودية, انتيروبكتر, ستروبكتر و الموركن , ولم نحصل على نمو بكتيريا في )%14.35(. ُدراسة الع قة بين التهاب المجاري البولية و سرطان النسيج البولي و وجد ان هنالك ع قة قوية بين ا ثنان وخصوصا مع أشريكية القولون, على الرغم من ان باقي البكتريا تلعب دورا ولكن بصورة اقل. جمع أشريكية القولون من داخل الخ يا السرطانية, يضاف الى ذلك الع قة القوية المت زمة مع النسيج السرطاني يجعلها من الممكن ان تكون من المسببات لذلك الورم السرطاني من خ ل قابليتها على انتاج اصابات حادة متكررة والتي تؤدي الى التهاباتحادة متكررة.


Article
Role of HEF1 Gene Expression in Prognosis of Urinary Bladder Transitional Cell Carcinoma

Authors: Athraa Falah Hasan --- Alaa Salah Jumaah
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2016 Volume: 13 Issue: 2 Pages: 354 -365
Publisher: Babylon University جامعة بابل

Loading...
Loading...
Abstract

Urinary bladder carcinoma is a common malignant tumor of the urogenital system worldwide. In 2015 ; it is the fourth most common cancer in men in the United State, while in Iraq it is one of the ten most common cancers in 2011. Human enhancer of filamentation 1 (HEF1) is a multidomain scaffolding protein of the Cas family; it is also an integral player in normal and pathological cell biology. The HEF1 protein has been implicated in the regulation of cell polarity, adhesion, motility, and invasion in multiple cell types. The main objective of the current study is to analyze HEF1 gene expression levels in urothelial carcinoma specimens and to study the impacts of HEF1 gene as genetic factors that contribute to development and prognosis of bladder cancer. Sixty samples of malignant bladder tumors as well as 60 samples of non-tumorous bladder tissues were investigated. Ages of patients were (62.95±12.839 s.d.) year. Total mRNA was extracted from FFPE blocks by using a specific kit. HEF1 gene expressions were estimated by using real-time PCR. Results were normalized to GAPDA gene as housekeeping gene. The gene expression data were analyzed in relevance to the patient's information obtained. Several statistical analyses were applied to analyze the data and found that the expression folds of HEF1 gene were found to be 11.219 folds in malignant bladder tumors in relation to non-tumorous bladder tissue. HEF1 genes were observed to be expressed excessively in high grade and advanced stage tumors which indicate that HEF1 gene may represent a novel bladder tumor marker with prognostic significance that could be introduced in plans of bladder cancer management.


Article
A study of p53 expression in transitional cell carcinoma of urinary bladder in Erbil governorate
دراسة تعبير p53 في سرطان الخلايا الانتقالية للمثانة في محافظة أربيل

Authors: Bakir Sadeq --- Salah A. Ali
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2012 Volume: 16 Issue: 3 Pages: 248-255
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

Loading...
Loading...
Abstract

Background and objectives: This study aimed to evaluate p53 protein expression in both normal bladder epithelium and in cases of transitional cell carcinoma (TCC) of urinary blad-der by immunohistochemical study and to correlate p53 expression in urothelial cancer with other clinico-pathological parameters.Methods: This is a retrospective and prospective study for sample collection during the period from January 2006-May 2009. The samples studied included 105 formalin fixed, paraffin embedded urinary bladder tissue specimens; they consisted of the following diag-nostic categories: chronic non specific cystitis (n=5) and urothelial cancer (n=100). In this study the nuclear p53 protein expression was detected in tissue samples by Dako Cytoma-tion. LSAB + System-HRP staining protocol using monoclonal mouse anti human protein DO-7.Results: None of the chronic non specific cystitis cases showed p53 nuclear immunostain-ing, while 93% of urothelial cancer specimens examined showed immunopositivity for p53 protein. In this study, a statistically significant correlation was observed between p53 over-expression rate with the tumor grade (p= <0.001) and histological architecture (p= 0.023), but not with other clinico-pathological parameters like age and gender.Conclusion: Results of the present study showed the validity and simplicity of application of immunohistochemistry in determining the status of p53 protein expression. The results suggest that p53 overexpression is strongly associated with the aggressiveness of urothe-lial cancer.


Article
Diagnostic value of immunohistochemical panel (Cytokeratin CK 7, Cytokeratin CK20, High molecular weight cytokeratin HMWCK (clone CK34βE12) and Prostatic specific antigen (PSA) in differentiation between poorly differentiated prostatic and urothelial carcinoma

Authors: Ikram A. Hasan --- Hiba A. Gaidan --- Methaq M. Al-kaabi
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2018 Volume: 11 Issue: 1 Pages: 7-13
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

Background: Prostatic adenocarcinoma may spread to bladder or vice versa, this is because of the anatomical proximity ofthese two organs. The differentiation between these two tumors is critical for therapeutic and prognostic implication.Aim of study: Evaluate the usefulness of a panel of immunohistochemical markers (CK7, CK20, HMWCK34 βE12 andPSA) in differentiation between challenging cases of high grade urothelial and poorly differentiated prostatic carcinoma withmorphological overlapping.Material and methods: A total of 40 cases (20 cases poorly differentiated prostatic adenocarcinoma and 20 cases high gradeurothelial carcinoma) were collected from archive of teaching laboratory of Al Yarmouk teaching hospital and private laboratoriesfor the period from January 2015 to July 2017.All formalin fixed paraffin embedded tissue block were stained immunohistochemicallywith a panel of marker (CK7, CK20, HMWCK34 βE12 and PSA) and scoring was performed.Results: For prostatic adenocarcinoma, 17 out 20 (85%) were positive for PSA, while only two cases (10%) of urothelial carcinomacases showed weak and focal staining for this marker (the pvalue was <0.0001). In contrast, 16 out of 20(80%) of theurothelial carcinoma cases were positive for CK34βE12 in comparison to only one case (5%)of prostatic carcinoma showedpositive expression for this marker (the p value was highly significant <0.0001).Regarding CK7and CK20: combined expression of both markers was noticed in 17 cases (85%) of urothelial carcinomacompared to only 2 cases(10%) of prostatic adenocarcinoma and the difference was highly significant(p value <0.0001). negativeexpression for both markers was noticed in 18 cases (90%) of prostatic adenocarcinoma compared to only 2 cases (10%)of urothelial carcinoma and the difference was highly significant( p value < 0.0001).CK7 positivity alone was noted in 17cases(85%) of urothelial carcinoma while only 2 cases (10%) of prostatic carcinoma show positivity for this marker and the pvalue was highly significant (p value < 0.0001).18 cases (90%) of urothelial carcinoma showed positive expression for CK20alone compared to only to 3 cases (15%) of prostatic carcinoma (p value < 0.0001).Conclusion: Using CK7 or CK20 alone will not be helpful for differentiation between prostatic carcinoma and high gradeurothelial carcinoma, while combined expression of both markers(CK7 and CK20) is very useful in ruling out carcinoma ofthe prostate, since, it is very rare for both markers to show positive expression in prostatic carcinoma.In difficult cases thatshow negative expression of both marker, CK34βE12 remain the most valuable marker for urothelial origin while, PSA immunohistochemicalmarker remains the most helpful marker to prove the prostatic origin of metastatic carcinoma.

Listing 1 - 4 of 4
Sort by
Narrow your search

Resource type

article (4)


Language

English (4)


Year
From To Submit

2018 (1)

2016 (1)

2015 (1)

2012 (1)