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Article
Effect of Vitamin E on Sperm Motility and Survival in Chilled-Stored Semen

Author: Ilaf H. Hadi
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: 22180265 Year: 2016 Volume: 6 Issue: 1 Pages: 1-7
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Sperm cells are well equipped with a powerful defense system of antioxidants, but an imbalance between the production of reactive oxygen species (ROS) and the available antioxidant-defenses result in oxidative stress. Therefore, antioxidants are supplemented extracellularly under in vitro conditions. Antioxidants are the main defense factors against oxidative stress induced by free radicals. Vitamin E is believed to be the primary component of the antioxidant system of the spermatozoa and is one of the major membrane protectants against ROS and lipid peroxidation (LPO) attack. It appears to be the first line of defense against the peroxidation of polyunsaturated fatty acids (PUFAs) contained in the cellular and sub-cellular membrane phospholipids because of its lipid solubility.Objective:The aim of the current study to evaluate effects of supplementing semen extender with vitamin E, at various concentrations (2, 6 and 12 IU/ml) on sperm motility, survival and morphology on the releases of free radicals and antioxidant enzymes within semen.Patients ,Materials and Methods:Twenty two patients were involved in the present study semen samples were obtained, and sperm count was assessed. The antioxidant (vitamin E) was formulated to be tested at three different levels as follow; 2, 6 and 12 IU/ml; Therefore, control and 3 antioxidant-containing extenders were prepared for semen dilution. The sample was divided into 4 aliquots. One volume of semen was added to 5 equal volumes of the designed extender. Extended semen samples (37¢ªC) were gradually cooled to 4¢ªC in the refrigerator and stored for 48 hours. A semen sample was taken out after 48h of storage, then warmed to 37¢ªC and checked for progressive motility, viability and abnormality.Sperm viability was assessed by Eosin Y. (0.5%).Nigrosin (0.1%) staining mixture. A total of 200 sperm were assessed under oil immersion with a high-resolution (X100) objective. Sperm morphology was assessed by Hematoxylin- Eosin satin, at least 200 sperm were scored on randomly chosen field, under oil immersion with a high-resolution (X100) objective.Results:The highest post-thaw motility and sperm survival (44% and 51.7%) 48 h after storage at 4¢ªC (Table 1) were attained (P < 0.01) in the basal diluents containing 6 IU vitamin E. Likewise, the correspondent percentages of live spermatozoa were 59%. Contrariwise, percentage of sperm abnormalities for the previous treatment was 25% significantly (P < 0.05) decrease compares with basal diluents containing 2, 12 IU vitamin E. The lowest (P ¡Ã 0.05) survival (23.5%, Table 1) was found in the basal diluents containing 12 IU vitamin. Beyond 6 IU vitamin E, there appears to exert adverse effects on sperm viability and survival.Conclusions:For the best protection against the increased free radicals production during chilling-preservation of human semen, extenders must contain 6 IU vitamin E per ml. The intensive production of free radicals in seminal plasma was counteracted by the inclusion of vitamin E at the above mentioned concentrations.

Keywords

vitamin E --- sperm --- extender.


Article
Antiplatelts Activity of Vitamin Ein Relation to Dose and Duration of Therapy

Authors: Shatha H. Ali شذى حسين علي --- Zaid O. Ibrahim
Journal: Iraqi Journal of Pharmaceutical Sciences المجلة العراقية للعلوم الصيدلانية ISSN: 16833597 Year: 2008 Volume: 17 Issue: 1 Pages: 25-30
Publisher: Baghdad University جامعة بغداد

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Abstract

Vitamin E, having the well known antioxidant activity through scavenging free radicals و it occurs in several isomeric forms , these isomers have relatively different functions . One of these actions is related to its ability to inhibit platelets aggregation and hence thrombosis. The present study included a total number of apparently healthy 62 males . 11of them served as standard group , treated with 100 mg aspirin /day for more than one month . Another 31 subjects were randomly grouped into 5 groups that received different daily doses of &#945; – tocopherol : 400 IU , 800 IU and 1200 IU for 2-6 months.The remainder ( 20 ) subjects served as a control group ( received no therapy ) . Platelets function was assessed based on measuring bleeding time and Slide Platelets Aggregation Time ( SPAT ) Meanwhile , thiobarbituric acid reactive substances (TBARS) were measured as a marker for oxidative stress. The results showed that the commercially available vitamin E preparations (&#945;- tocopherol ) could exert anti-coagulant effect ,such effect is more dependant on duration of therapy , rather than dose related action .In addition to it’s antioxidant effect, which seems to be significantly correlated to it’s antiplatelets effect ( r=0.994 ,p<0.05).Hence , long term administration of high doses of vitamin E could be effective in decreasing the incidence of thrombosis ,which in turn depends on platelets function. Such effect might not affect bleeding time obviously , but it could reduce chances for platelets recruitment , which might represent an additional advantageous action for vitamin E over other antioxidants.

ان فيتامين أي معروف بخاصيته المضادة للاكسدة من خلال اقتناصه للجذور الحرة المتكونة .ان هذه الجزيئة توجد في عدة صور جزيئية وهذه الاشكال المختلفة للجزيئة تميز كل منها بخصائص ووضائف مختلفة .احدها يرتبط باحباط قابلية الصفيحات الدموية للتكتل وتكوين الخثرة الدموية .تضمنت هذه الدراسة 62 ذكرا سليما ، اعتبر احد عشر منهم مجموعة قياسية للمقارنة حيث تم علاجها بالاسبرين 100 ملغم يوميا ولمدة اكثر من شهر واحد . وتم تقسيم 31 شخصا اخر وبصورة عشوائية الى مجاميع تتناول الفا – توكوفيرول بجرع مختلفة ( 400 و800 و1200 وحدة دولية يوميا ) ولمدد مختلفة تراوحت بين الشهرين والستة أشهر . اما باقي المشاركين بالدراسة فقد تم اعتبارهم مجموعة مقارنة لم يتناولوا أي علاج .تم اعتماد الفحوصات المختبرية الخاصة بفعالية الصفيحات الدموية مثل قياس مدة النزف و الوقت اللازم للصفيحات الدموية للتجمع على السلايداضافة الى قياس مستوى الموادالمتفاعلة مع حامض الثايوباربيجورك كدليل لدرجة الاجهاد التاكسدي.اظهرت النتائج ان النوع المتوفر في اسواق العراق من الفيتامين E وهو في الاغلب يتكون من نوع الفا توكوفيرول فقط يمكن ان يحدث تأثيرات على عملية تجلط الدم . وان هذا التأثير فيما يبدو يعتمد بصورة اكبر على مدة استمرار العلاج اكثر من ارتباطه بالجرعة المعطاة من هذا الفيتامين .لذلك ان تناول فيتامين أي -الفا توفيرول – وبجرع كبيرة نسبيا ولمدة طويلة –اكثر من خمسة اشهر – يمكن ان يعطي تأثيرا مفيدا من خلال تقليل احتمالية الاصابة بتخثرات الدم من خلال تأثيرها على فعالية الصفيحات الدموية وهذا التأثير الايجابي يمكن ان يكون تأثيرا اضافيا لتفضيل فيتامين أي على غيره من المواد المضادة للاكسدة .


Article
Antibacterial Effects of Vitamin E: in Vitro Study
تأثير فيتامين هـ المضاد للبكتريا: دراسة خارج الكائن الحي

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Abstract

Overuse of antibiotics has become the major factor for the emergence and dissemination of multi-drug resistant strains of several groups of microorganisms and this lead to search for agents that may have antibacterial effects. Vitamin E emerged as an essential, fat-soluble nutrient in the human body and it is essential, because the body cannot manufacture its own vitamin E, so foods and supplements must provide it. The aim of the present study was to evaluate the effect of vitamin E against pathogenic bacteria. Gram positive and gram negative bacteria were selected as the test microorganisms based on their importance in infections. In this study vitamin E used in four concentrations (50,100,200,400) IU/ml. The agar diffusion method was used to determine antibacterial activity. Results showed that gram negative bacteria were shown to be more resistant than gram positive bacteria. The resistance of gram negative bacteria towards antibacterial substances may be related to lipopolysaccharides in their outer membrane.

كثرة استخدام المضادات الحيوية اصبحت السبب الرئيسي لانتشار سلالات بكتيرية مقاومة لهذه المضادات مما دفع الباحثين لايجاد مواد اخرى ذات تاثير مضاد للبكتريا. فيتامين هـ هو فيتامين ضروري ولايستطيع جسم الانسان تصنيعه بل يحصل عليه من الطعام. الهدف من الدراسة هو تقييم تاثير فيتامين هـ على عدة انواع من البكتيريا المرضية. البكتريا الموجبة لصبغة كرام والبكتريا السالبة لصبغة كرام استخدمت في هذه الدراسة، فيتامين هـ استخدم باربع تراكيز (50،100،200،400IU/m(l وطريقة الانتشار بالحفر استخدمت لمعرفة التاثير المضاد للبكتريا. اظهرت النتائج ان البكتريا السالبة اكثر مقاومة لتاثير فيتامين هـ من البكتريا الموجبة.

Keywords

Antibacterial --- Vitamin E --- in vitro


Article
Vitamin E Level In Friedreich’s Ataxic Phenotype Patients In Four Major Hospitals In Baghdad

Author: Ahmed Hasan Ahmed*, Ahmed Tahseen Muslim**, Basim Hanoon Jabbar** , Zaki Noah Hasan***.
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2017 Volume: 13 Issue: 1 Pages: 132-136
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Friedreich ataxia (FRDA) is the most common form of inherited ataxia, comprising one-half of all hereditary ataxias with a carrier rate between 1 in 60 to 1 in 90 and with a disease prevalence of 1 per 29,000. It can occur in two forms the classic form or in association with a vitamin E dependent ataxia. The precise role of Vitamin E in the nervous system is unknown; An Oxidative attack is suspected to play a role in Ataxia with Vitamin E deficiency, as well as in Friedreich ataxia. Vitamin E is the major free-radical-trapping antioxidant.Objective: The objectives of the study is to asses vitamin E level in patients with Friedreichs ataxia phenotype in Iraqi patients. Type of the study:Cross-sectional study.Method: This study was conducted at the neuroscience hospital and Baghdad teaching hospital during the period from the 1st of November 2013 through November 2014. Forty patients with friedreich's ataxia attended in neuroscience hospital and Baghdad teaching hospital during this period; there was12 male, 20 female patients and their age range between (4-50) years. Results: Regarding the level of vitamin E in patients in the present study, The study revealed that mean level of vitamin E for Friedreich ataxia patients was (10.92 μg/ml) ranging from (8-18 μg/ml),while in the control group the mean was (28.06μg/ml) ranging from (22-36μg/ml), the difference in mean level was found to be statistically significant (p=0.0001.) Thirty percents of (FRDA) patients was (8-9.9μg/ml), while half of patients (50%) were within (10-11.9 μg/ml),and low percentage (15 %)of patients were within (12-13.9 μg/ml ). The rest (5%) were had vitamin E level equal or more than 14 μg/ml. these finding revealed that majority of Friedreich ataxia patients were found with low level of vitamin E (32 patients(80%)), and low percentage with normal level (8 patients(20%.)) Patients with Friedreich ataxia were found to have a comparable level 10.47±1.79(8.4-13.5),11.48±2.61(9.4-18) and 10.66±1.26(9-12.5) for those with less than 10 years, 10-19 years , equal or more than 20 years of age respectively (p=0.382), while it was found to have a significantly decreasing level with advance in the age for the control group, 30.57±4.61(24.5-36), 27.46±3.16(23.7-33.5), 25.50±3.03 (22.9-29) for those less than 10 years, 10-19 years, 20 years or more respectively (p=0.006.) Vitamin E level in relation to Gender showed that Friedriech ataxia male patients had significantly higher vitamin E level compared to females [12.05±2.66 (9.0-18.0) compared to 10.17±1.07 (8.4-12.0)] (p=0.003), while relation to gender in the control group was not of significant value statistically [27.08±2.90 (22.9-30.0) for male compared to 29.04±4.97 (23.7-36) for females] , p=0.136. Conclusion: In the light of the results of the present study, the following conclusions were made: The level of vitamin E in friedreichs ataxic phenotype patients was subnormal than normal range representing 80% of the sample selected for the test. The level of vitamin E in male patients of freidriechs ataxic phenotype patients was higher than female patients.


Article
SEM Study on Cytotoxic effect of Monocrotophos (MCP) on Blood of Mice

Author: Khalid Hamdan Gathwan
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 1 Pages: 36-41
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACK GROUND: Monocrotophos (MCP) is an organophosphorus insecticide has both systemic and contact actions and has been used against insects. METHODS: Thirty six male Balb/c mice were used. These were divided into six group (A, B, A1, B1, A2, B2, ). MCP was given orally daily by gavage 1/5th of LD50 (0.28 mg/100gm of body wt.), vitamin E and vitamin C was given orally daily by gavage @ 100 mg /kg body wt., while MCP+ Vitamine E and MCP + Vitamine C was also given. RESULTS: The signs of MCP toxicity include shivering, salviation and Iacrimation. Scanining electron microscopy (SEM) revealed that of erythrocytes in control were perfect discocytes (D). MCP treatment resulted in drastic alteration in the topography of erythrocytes. Vitamin E and Vitamin C treated mice revealed normal erythrocyte like that in control. MCP+ Vitamin E and MCP + vitamin C treated mice revealed almost normal of topography of erythrocyte. CONCLUSION: Pesticide exposure could be prevented by CO- administration antioxidant Vitamin E or Vitamin C.

Keywords

MCP --- Vitamin E --- Vitamin C --- Erythrocytes


Article
PROTECTIVE ROLE OF VITAMIN E OR/AND METHIONINE AGAINST LEAD-INDUCE CHANGES ON HEMATOLOGICAL PARAMETERS IN RABBITS

Authors: Majeda AJ Alqayim ماجدة عبد الخالق القيم --- Sadat A Asis سادات عبد العزيز
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 2 Pages: 187-194
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:The importance of lead as a toxic metal and environmental pollutant has long been recognized to human and animal health. In Iraq, lead pollution was documented in Baghdad and in Sulaimaniya city.Objective:To explore the protective role of vitamin E alone or in combination with the amino acid methionine against lead acetate side effects on hematological parameters of adult male rabbits.Methods:Thirty male adult rabbits were divided equally into five groups four of them administered lead acetate for 90 days, as sub-chronic exposure, and the fifth was considered as control. Three of these groups were treated with Vitamin E and/or methionine for 90 days. At the end of experiment, blood and liver samples were collected for either hematological analysis or histopathological examination.Results:Lead caused a significant decrease in lymphocytes and erythrocyte indices; and a significant increase in reticulocytes and neutrophils. At the same time, Vit. E, alone or mixed with methionine, corrected these values to semi normal values.Conclusion:Decreased erythrocyte indices and reticulocytosis that is found in the present study, demonstrates regenerative anemia in rabbits that had administered lead acetate. Vitamin E, alone or mixed with methionine, was efficient in reducing the side effects of Lead on hematological parameters; while, methionine had little or no effect when administered alone against lead which may be attributed to the dose or duration of the treatment.Keywords:Lead; vitamin E; methionine; hematological changes


Article
The protective impact of vitamin E against atenolol effect on Ca+2, Testosteron and FSH in male rats

Authors: Safa M. Abbas --- Luma W.Khalil
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2016 Volume: 7 Issue: 2 Pages: 151-157
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background and objective: Atenolol is β1-selective antagonist nonetheless this selectivity is not absolute and at higher dosages atenolol represses β-2 Adrenoceptors mainly located in bronchial and vascular musculature, alsoβ-blocker atenolol effects on testosterone and FSH production. Therefore ,this study was designed to investigate the possible protective effect of vitamin E on reproductive efficiency in male rats exposed to atenolol.Materials and methods:Thirty-two healthy adult males Wistar rats at random divided into four equal groups and treatment for 56 days as follows:-First group (control):Animals in this group received tap water and served as control.Second group (group T1):Animal in this group will be receive atenolol orally at dose (100mg/kg/day).Third group (group T2):Animal in this group will be receive Vit.E (800IU /B.W.) orally and Fourth group (group T3):Animal in this group will be receive Vit.E (800IU/B.W.) and atenolol at dose (100mg/kg/B.W.) orally. Results: serum level of serum testosterone in control group was 1.51±0.21 ng/ml, and it significantly decreased in atenolol treated group (0.74±0.008 ng/ml),.Serum level of FSH in the control group was (4.87±0.03 mIU /ml) while it significantly reduced in atenolol treated group (2.69±0.15mIU/ml), also the results showed that atenolol was a significantly decrease the Ca+2 concentration to (1.20 ± 0.15 mg/dl) in contrast to control group. Conclusion: Vitamin E improve significantly the levels of serum FSH and testosterone in in atenolol treated rats.


Article
Docetaxel Chemotherapy Induced Peripheral Neuropathy in Breast cancer Patients and its Amelioration by Vitamin E

Authors: Adel Hamid Farhan --- Ammar Rasoul Mohammad عمار رسول محمد --- Hayder Yousif Hameed حيدر يوسف حميد --- Talib Hussein Kamoona طالب حسين كمونة
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2017 Volume: 17 Issue: 1 Pages: 13-27
Publisher: University of Kufa جامعة الكوفة

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Abstract

Background: Chemotherapy is used to treat different types of cancer. Its use is associated with neurotoxicity, the most common of them is peripheral neuropathy. Taxanes are recognized to cause peripheral neuropathy of which Docetaxel is studied.Aim: To evaluate the neurotoxicity of Docetaxel and assess the protective effect of vitamin E in breast cancer patients.Methods: In a prospective placebo controlled randomized study, 60 women with breast cancer were selected. Patients had surgery and planned for chemotherapy administration. Chemotherapy protocol used is TAC that includes Docetaxel, Doxorubicin and Cyclophosphamide given every 21 days. They are assessed for neuropathy clinically and by nerve conduction study at the baseline and after completion of chemotherapy. They are divided into 2 groups each composed of 30 patients. The 1st group is given Vitamin E and the second group is given placebo.Results: showed that the incidence of neuropathy is the same in both groups, but it was less severe in Vitamin E treated group compared to the placebo group.Conclusion: vitamin E ameliorates the severity of peripheral neuropathy and can be used for this purpose.Recommendation: We recommend large multicenter studies to be done and using other agents that possibly prevent or ameliorate Docetaxel induced neuropathy in the future.


Article
Nephroprotective effect of vitamin E added to angiotensin receptor blocker in patients with diabetic nephropathy

Authors: Anwar Ghani Almosewi انور غني الموسوي --- Sabah Ali Alhelu صباح علي الحلو --- Abdulrazzaq Hassan Alkaaby عبد الرزاق حسن الكعبي
Journal: KUFA MEDICAL JOURNAL مجلة الكوفة الطبية ISSN: 1993517X Year: 2017 Volume: 17 Issue: 1 Pages: 55-61
Publisher: University of Kufa جامعة الكوفة

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Background: Diabetes mellitus is a condition associated with increased oxidative stress as a consequence of hyperglycemia. Therefore, the use of antioxidants in people with diabetes has been advocated. Vitamin E is the most prevalent naturally occurring anti-oxidant.Aim: To evaluate the effect of the combination of angiotensin receptor blocker (ARB) and vitamin E on urine protein in patients with diabetic nephropathy.Methods: One hundred and six patients with diabetic nephropathy, who visited the Alsader medical city in Najaf governorate and Al-Furat Al-Awsat general hospital in Kufa, were investigated, 39 of them are type 1diabetics, and 67 of them are type 2 diabetics. Each patient was followed up for 3 months with valsartan (160mg/day), then for 3 months with addition of tocopherol (400mg). At the screening visits, proteinuria was determined in two 24 h urine sample, arterial blood pressure was measured twice at ten minutes rest with 2 minutes intervaland serum level of potassium, sodium & creatinine were determined.Results: Proteinuria was significantly lowered when tocopherol 400mg was added to valsartan, as compared with valsartan without tocopherol. Valsartan alone caused a mean reduction of 24 h urine protein of 27% from the baseline, while the addition of tocopherol caused a mean reduction of 24 hour proteinuria of 30% from result of valsartan. That is mean the combination therapy of valsartan and tocopherol caused a reduction in proteinuria of 44% from baseline.Conclusion: The current study suggests that combination therapy of ARB and vitamin E offers an additional antiproteinuric and nephroprotective effect.Recommendation: Antioxidants, in particular vitamin E have promising nephroprotective effects. However, studies on other antioxidants like vitamin C are needed in the efforts of prevention of diabetic nephropathy.


Article
Evaluation of Estridiol and Some Antioxidant in Breast Cancer Iraqi Women

Authors: Ghufran Saad Nsaif --- Amer Hassan Abdallah --- Najwa Shehab Ahmed --- Wafaa Raji Alfatlawi
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2018 Volume: 21 Issue: 1 Pages: 35-40
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Cancer is the second reason of deaths worldwide while breast cancer is the more spread cancer in women, comprising 29 % from all cancers that they are affected Samples were obtained from patients undergoing surgery in center from breast cancer in oncology teaching hospital Al-Eluia hospital for woman care All blood samples were taken of the patients (110 women) and divided into four groups benign, malignant, radiotherapy and control group In order to measurement estrogen levels which are influenced by the menstrual cycle, the main groups were furthermore divided into sub-groups, premenopausal and postmenopausal women The mean value ± SE of Body Mass Index (BMI) for control, benign, malignant and radiotherapy breast cancer were showed a non-significant change in the comparison of groups according to the Duncan test Estrogen were increased before therapy but decreased following therapy The levels of malondialdehyde (MDA)were elevated in patients after radiotherapy The mean value ± SE of vitamin E were decreased in studied groups Present study aims to study the effects of serum estrogen, vitamin E and antioxidant status in breast cancer patients

Keywords

Estrogen --- Vitamin E --- MDA --- BMI --- Breast cancer

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