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This study was conducted in the Animal Farm, College of Agriculture, University of Baghdad, from march to may 2013, to clarify the effect of subcutaneous vitamin C injection on semen characteristics of Awassi rams. Six rams (1.5-2 years old) were randomly divided into three groups of 2 rams. Rams in T1and T2 were subcutaneously injected, every other day with dose of 50 and100 mg/kg body weight respectively ,for 45 days while control group in T0 received normal saline. The results showed a significant increase (P<0.05) in the The sperm concentration of T1 as compared with control group, while there were no significant differences among treated groups(T1 and T2). Group 2 showed a significant (P<0.05) increases in mass activity, individual motility and live sperm percentage. There was a significant decrease in individual motility and percentage of live sperm in T2 as compared to T1 and control group.In conclusion, despite of the significant improvement of some semen trail as in T2 group, the lower dose of vit. C(less than 50 mg/kg of body weight) was recommended represented the skin tearing(T2group) as well as the redness and sensitive skin in T1 group.

words:Vitamin C --- Semen --- Rams